ntllg4介导的NtPPME1的非常规极性胞分泌协调细胞壁刚性和膜动力学,控制花粉管完整性

IF 12.5 1区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
Xun Weng, Hao Wang (王昊), Yifeng Jiang, Ziheng Wang, Zhiheng Chen, Chuanhao Liu, Zhiyuan Yang, Jiayang Gao, Liwen Jiang, Lifeng Zhao, Jilei Huang, Feng Zhang, Hao Wang (王浩)
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引用次数: 0

摘要

植物受精依赖于控制花粉管生长,结合膜动力学和细胞壁扩张。我们之前发现了一种非常规的胞外通路,其中高尔基衍生分泌囊泡(gdsv)绕过反式高尔基网络递送烟草果胶甲基酯酶1 (NtPPME1),从而调节细胞壁刚性。然而,将这一途径与膜动力学和信号传导联系起来的机制仍然难以捉摸。在这里,我们使用低温聚焦离子束扫描电子显微镜和三维断层扫描来鉴定gdsv是花粉管尖端的一个独特的囊泡群体。我们进一步证明,烟草lorelei样糖基磷脂酰肌醇锚定蛋白4 (NtLLG4)是控制膜动力学和完整性的关键信号分子,可作为NtPPME1的受体,通过gdsv调节其极性胞分泌,从而控制细胞壁刚度。此外,我们还发现了介导NtPPME1跨细胞器非常规胞外分泌的运输信号。我们的发现揭示了细胞壁刚性与膜信号耦合的关键机制,以控制受精过程中花粉管的生长和完整性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

NtLLG4-mediated unconventional polar exocytosis of NtPPME1 coordinates cell wall rigidity and membrane dynamics to control pollen tube integrity

NtLLG4-mediated unconventional polar exocytosis of NtPPME1 coordinates cell wall rigidity and membrane dynamics to control pollen tube integrity
Plant fertilization relies on controlled pollen tube growth that integrates membrane dynamics and cell wall expansion. We previously identified an unconventional exocytic pathway wherein Golgi-derived secretory vesicles (GDSVs) bypass the trans-Golgi network to deliver Nicotiana tabacum pectin methylesterase 1 (NtPPME1), thereby modulating cell wall rigidity. However, the mechanisms linking this pathway with membrane dynamics and signaling remain elusive. Here, we used cryo–focused ion beam–scanning electron microscopy and three-dimensional tomography to identified GDSVs as a distinct vesicle population at the pollen tube tip. We further demonstrated that tobacco LORELEI-like glycosylphosphatidylinositol-anchored protein 4 (NtLLG4), a key signaling molecule controlling membrane dynamics and integrity, functions as a receptor for NtPPME1, regulating its polar exocytosis via GDSVs to control cell wall stiffness. Furthermore, we identified trafficking signals that direct the unconventional exocytosis of NtPPME1 across intracellular organelles. Our findings reveal a crucial mechanism coupling cell wall rigidity with membrane signaling to control pollen tube growth and integrity during fertilization.
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来源期刊
Science Advances
Science Advances 综合性期刊-综合性期刊
CiteScore
21.40
自引率
1.50%
发文量
1937
审稿时长
29 weeks
期刊介绍: Science Advances, an open-access journal by AAAS, publishes impactful research in diverse scientific areas. It aims for fair, fast, and expert peer review, providing freely accessible research to readers. Led by distinguished scientists, the journal supports AAAS's mission by extending Science magazine's capacity to identify and promote significant advances. Evolving digital publishing technologies play a crucial role in advancing AAAS's global mission for science communication and benefitting humankind.
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