Ning Wang, Ting Wang, Mengting Fan, Chen Li, Yue Tian, Xiaoyan Cui
{"title":"利用纳米聚合对14组罗丹明进行超高信噪比标记的一般策略","authors":"Ning Wang, Ting Wang, Mengting Fan, Chen Li, Yue Tian, Xiaoyan Cui","doi":"10.1002/agt2.70077","DOIUrl":null,"url":null,"abstract":"<p>Ultrahigh signal-to-noise ratio (SNR) labeling enables precise visualization of biological structures in vivo. We boosted fluorogenicity in group-14-rhodamines by comprehensively manipulating their dynamics in physical (aggregate/monomer, <i>K</i><sub>A/M</sub>) and chemical (closed/open spirolactone, <i>K</i><sub>C/O</sub>) states. Fluorogenic rhodamines were designed by group 14 (C, Si, Ge) substituted bridging regions in xanthene with tuned dialkylation. We quantified the impact of alkylation with the hydrophobicity (log<i>P</i>) over a wide range and confirmed that SNR can be sharply improved, owing to the promoted nano-aggregation (<i>K</i><sub>A/M</sub>) with high log<i>P</i>. Integrating <i>K</i><sub>A/M</sub> with <i>K</i><sub>C/O</sub> mechanisms, unparalleled fluorogenicity was observed in group-14-rhodamines: HaloTag probe with dipentylsilyl exhibits remarkable fluorogenicity (>2000) in vitro, enabling no-wash and multicolor super-resolution stimulated emission depletion imaging of high SNR (>300) in vivo. Overexpression of <i>α</i><sub>v</sub><i>β</i><sub>3</sub> was sensitively tracked in vivo by RGDyK-based fluorogenic SiR probe through tuned <i>K</i><sub>A/M</sub>. Our proposed strategy has significantly promoted the fluorogenicity of group 14 rhodamines as a general mechanism.</p>","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"6 8","pages":""},"PeriodicalIF":13.7000,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.70077","citationCount":"0","resultStr":"{\"title\":\"A General Strategy to Fine-Tune Group 14 Rhodamines for Ultrahigh Signal-to-Noise Ratio Labeling In Vivo by Nano-Aggregation\",\"authors\":\"Ning Wang, Ting Wang, Mengting Fan, Chen Li, Yue Tian, Xiaoyan Cui\",\"doi\":\"10.1002/agt2.70077\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Ultrahigh signal-to-noise ratio (SNR) labeling enables precise visualization of biological structures in vivo. We boosted fluorogenicity in group-14-rhodamines by comprehensively manipulating their dynamics in physical (aggregate/monomer, <i>K</i><sub>A/M</sub>) and chemical (closed/open spirolactone, <i>K</i><sub>C/O</sub>) states. Fluorogenic rhodamines were designed by group 14 (C, Si, Ge) substituted bridging regions in xanthene with tuned dialkylation. We quantified the impact of alkylation with the hydrophobicity (log<i>P</i>) over a wide range and confirmed that SNR can be sharply improved, owing to the promoted nano-aggregation (<i>K</i><sub>A/M</sub>) with high log<i>P</i>. Integrating <i>K</i><sub>A/M</sub> with <i>K</i><sub>C/O</sub> mechanisms, unparalleled fluorogenicity was observed in group-14-rhodamines: HaloTag probe with dipentylsilyl exhibits remarkable fluorogenicity (>2000) in vitro, enabling no-wash and multicolor super-resolution stimulated emission depletion imaging of high SNR (>300) in vivo. Overexpression of <i>α</i><sub>v</sub><i>β</i><sub>3</sub> was sensitively tracked in vivo by RGDyK-based fluorogenic SiR probe through tuned <i>K</i><sub>A/M</sub>. Our proposed strategy has significantly promoted the fluorogenicity of group 14 rhodamines as a general mechanism.</p>\",\"PeriodicalId\":72127,\"journal\":{\"name\":\"Aggregate (Hoboken, N.J.)\",\"volume\":\"6 8\",\"pages\":\"\"},\"PeriodicalIF\":13.7000,\"publicationDate\":\"2025-06-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.70077\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Aggregate (Hoboken, N.J.)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/agt2.70077\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aggregate (Hoboken, N.J.)","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/agt2.70077","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
A General Strategy to Fine-Tune Group 14 Rhodamines for Ultrahigh Signal-to-Noise Ratio Labeling In Vivo by Nano-Aggregation
Ultrahigh signal-to-noise ratio (SNR) labeling enables precise visualization of biological structures in vivo. We boosted fluorogenicity in group-14-rhodamines by comprehensively manipulating their dynamics in physical (aggregate/monomer, KA/M) and chemical (closed/open spirolactone, KC/O) states. Fluorogenic rhodamines were designed by group 14 (C, Si, Ge) substituted bridging regions in xanthene with tuned dialkylation. We quantified the impact of alkylation with the hydrophobicity (logP) over a wide range and confirmed that SNR can be sharply improved, owing to the promoted nano-aggregation (KA/M) with high logP. Integrating KA/M with KC/O mechanisms, unparalleled fluorogenicity was observed in group-14-rhodamines: HaloTag probe with dipentylsilyl exhibits remarkable fluorogenicity (>2000) in vitro, enabling no-wash and multicolor super-resolution stimulated emission depletion imaging of high SNR (>300) in vivo. Overexpression of αvβ3 was sensitively tracked in vivo by RGDyK-based fluorogenic SiR probe through tuned KA/M. Our proposed strategy has significantly promoted the fluorogenicity of group 14 rhodamines as a general mechanism.
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