Heba Abuzaied, Dina W. Bashir, Eman Rashad, Maha M. Rashad, Basma M. Bawish, Hany El-Habback
{"title":"大自然的盾牌：人参是一种神经保护剂，可以防止二氧化钛纳米颗粒的暴露","authors":"Heba Abuzaied, Dina W. Bashir, Eman Rashad, Maha M. Rashad, Basma M. Bawish, Hany El-Habback","doi":"10.1007/s10735-025-10561-w","DOIUrl":null,"url":null,"abstract":"<div><p>Titanium dioxide nanoparticles (TiO₂-NPs) are widely used in various industries due to their unique properties. However, they can have harmful effects on biological systems. <i>Panax ginseng</i> is a popular natural remedy known for its protective effects against several health conditions. This study investigates the protective and therapeutic effects of ginseng against TiO₂-NPs-induced neurotoxicity in male rats. Thirty-five adult male albino rats were assigned to five groups: the untreated control, ginseng (received 200 mg/kg bw ginseng), TiO<sub>2</sub>-NPs-treated (received 200 mg/kg bw TiO<sub>2</sub>-NPs), ginseng-protected (received 200 mg/kg bw ginseng + 200 mg/kg bw TiO<sub>2</sub>-NPs), and ginseng-treated (received 200 mg/kg bw of TiO<sub>2</sub>-NPs for 14 days, then 200 mg/kg bw of ginseng till the end of the experiment) groups. All treatments were administered orally by gavage for 30 days. After the final dose, rats underwent behavioural assessments. Brain tissues were then collected for biochemical, histological, and immunohistochemical analysis. The cognitive capacity, emotional reactivity, and spatial recognition of rats were significantly impaired by exposure to TiO<sub>2</sub>-NPs. TiO<sub>2</sub>-NPs elevated malondialdehyde (MDA) levels, while simultaneously causing a dramatic reduction in glutathione (GSH) levels. Moreover, TiO<sub>2</sub>-NPs caused significant histological changes, including vacuolation of the neuropil and neuronal deterioration. Additionally, treatment with TiO<sub>2</sub>-NPs induced a highly positive glial fibrillary acidic protein (GFAP) immunoreaction. Conversely, ginseng administration improved behavioural performance, restored antioxidant levels, and reduced tissue damage. These results suggest that ginseng may offer protective and therapeutic benefits against TiO₂-NP-induced brain toxicity.</p></div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 5","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Nature’s shield: ginseng as a neuroprotective agent against titanium dioxide nanoparticle exposure\",\"authors\":\"Heba Abuzaied, Dina W. Bashir, Eman Rashad, Maha M. 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All treatments were administered orally by gavage for 30 days. After the final dose, rats underwent behavioural assessments. Brain tissues were then collected for biochemical, histological, and immunohistochemical analysis. The cognitive capacity, emotional reactivity, and spatial recognition of rats were significantly impaired by exposure to TiO<sub>2</sub>-NPs. TiO<sub>2</sub>-NPs elevated malondialdehyde (MDA) levels, while simultaneously causing a dramatic reduction in glutathione (GSH) levels. Moreover, TiO<sub>2</sub>-NPs caused significant histological changes, including vacuolation of the neuropil and neuronal deterioration. Additionally, treatment with TiO<sub>2</sub>-NPs induced a highly positive glial fibrillary acidic protein (GFAP) immunoreaction. Conversely, ginseng administration improved behavioural performance, restored antioxidant levels, and reduced tissue damage. 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Nature’s shield: ginseng as a neuroprotective agent against titanium dioxide nanoparticle exposure
Titanium dioxide nanoparticles (TiO₂-NPs) are widely used in various industries due to their unique properties. However, they can have harmful effects on biological systems. Panax ginseng is a popular natural remedy known for its protective effects against several health conditions. This study investigates the protective and therapeutic effects of ginseng against TiO₂-NPs-induced neurotoxicity in male rats. Thirty-five adult male albino rats were assigned to five groups: the untreated control, ginseng (received 200 mg/kg bw ginseng), TiO2-NPs-treated (received 200 mg/kg bw TiO2-NPs), ginseng-protected (received 200 mg/kg bw ginseng + 200 mg/kg bw TiO2-NPs), and ginseng-treated (received 200 mg/kg bw of TiO2-NPs for 14 days, then 200 mg/kg bw of ginseng till the end of the experiment) groups. All treatments were administered orally by gavage for 30 days. After the final dose, rats underwent behavioural assessments. Brain tissues were then collected for biochemical, histological, and immunohistochemical analysis. The cognitive capacity, emotional reactivity, and spatial recognition of rats were significantly impaired by exposure to TiO2-NPs. TiO2-NPs elevated malondialdehyde (MDA) levels, while simultaneously causing a dramatic reduction in glutathione (GSH) levels. Moreover, TiO2-NPs caused significant histological changes, including vacuolation of the neuropil and neuronal deterioration. Additionally, treatment with TiO2-NPs induced a highly positive glial fibrillary acidic protein (GFAP) immunoreaction. Conversely, ginseng administration improved behavioural performance, restored antioxidant levels, and reduced tissue damage. These results suggest that ginseng may offer protective and therapeutic benefits against TiO₂-NP-induced brain toxicity.
期刊介绍：
The Journal of Molecular Histology publishes results of original research on the localization and expression of molecules in animal cells, tissues and organs. Coverage includes studies describing novel cellular or ultrastructural distributions of molecules which provide insight into biochemical or physiological function, development, histologic structure and disease processes.
Major research themes of particular interest include:
- Cell-Cell and Cell-Matrix Interactions;
- Connective Tissues;
- Development and Disease;
- Neuroscience.
Please note that the Journal of Molecular Histology does not consider manuscripts dealing with the application of immunological or other probes on non-standard laboratory animal models unless the results are clearly of significant and general biological importance.
The Journal of Molecular Histology publishes full-length original research papers, review articles, short communications and letters to the editors. All manuscripts are typically reviewed by two independent referees. The Journal of Molecular Histology is a continuation of The Histochemical Journal.
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