Kihyun Kim, Sohyun Park, Suyoung Kang, Mi-Kyung Lee, Lingxin Chen, Jaebum Choo
{"title":"基于sers的体外培养检测大肠杆菌在尿路感染诊断中的应用","authors":"Kihyun Kim, Sohyun Park, Suyoung Kang, Mi-Kyung Lee, Lingxin Chen, Jaebum Choo","doi":"10.1186/s40580-025-00506-0","DOIUrl":null,"url":null,"abstract":"<div><p>A surface-enhanced Raman scattering (SERS)-based aptasensor was developed for the rapid and sensitive detection of <i>Escherichia coli</i> (<i>E. coli</i>), a major pathogen in urinary tract infections (UTIs). The sensor utilizes magnetic beads embedded with gold nanoparticles (MB-AuNPs) functionalized with capture DNA (cDNA) as both the SERS-active substrate and magnetic separation tool. The detection mechanism relies on an aptamer DNA-probe DNA complex: when the aptamer binds specifically to <i>E. coli</i>, the probe DNA is released and subsequently hybridizes with cDNA on the MB-AuNPs. This brings a Cy5 Raman label close to the gold surface, generating a strong SERS signal. The assay offers a one-step process, eliminating the need for bacterial culture or nucleic acid amplification, and completes within approximately 6 h. Quantitative analysis demonstrated a detection limit of 5.9 × 10<sup>3</sup> CFU/mL, well below the clinical threshold for UTIs, with a reliable calibration curve (R<sup>2</sup> = 0.990). Selectivity tests confirmed high specificity for <i>E. coli</i> without cross-reactivity to other bacteria. Clinical evaluation using 21 urine samples showed high diagnostic performance: 100% sensitivity, 91% specificity, 95% accuracy, and 100% precision compared to standard urine culture. These results highlight the aptasensor’s potential as a rapid, sensitive, and specific alternative for UTI diagnosis in clinical settings.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":712,"journal":{"name":"Nano Convergence","volume":"12 1","pages":""},"PeriodicalIF":11.0000,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://nanoconvergencejournal.springeropen.com/counter/pdf/10.1186/s40580-025-00506-0","citationCount":"0","resultStr":"{\"title\":\"SERS-based aptasensor for culture-free detection of Escherichia coli in urinary tract infection diagnosis\",\"authors\":\"Kihyun Kim, Sohyun Park, Suyoung Kang, Mi-Kyung Lee, Lingxin Chen, Jaebum Choo\",\"doi\":\"10.1186/s40580-025-00506-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A surface-enhanced Raman scattering (SERS)-based aptasensor was developed for the rapid and sensitive detection of <i>Escherichia coli</i> (<i>E. coli</i>), a major pathogen in urinary tract infections (UTIs). The sensor utilizes magnetic beads embedded with gold nanoparticles (MB-AuNPs) functionalized with capture DNA (cDNA) as both the SERS-active substrate and magnetic separation tool. The detection mechanism relies on an aptamer DNA-probe DNA complex: when the aptamer binds specifically to <i>E. coli</i>, the probe DNA is released and subsequently hybridizes with cDNA on the MB-AuNPs. This brings a Cy5 Raman label close to the gold surface, generating a strong SERS signal. The assay offers a one-step process, eliminating the need for bacterial culture or nucleic acid amplification, and completes within approximately 6 h. Quantitative analysis demonstrated a detection limit of 5.9 × 10<sup>3</sup> CFU/mL, well below the clinical threshold for UTIs, with a reliable calibration curve (R<sup>2</sup> = 0.990). Selectivity tests confirmed high specificity for <i>E. coli</i> without cross-reactivity to other bacteria. Clinical evaluation using 21 urine samples showed high diagnostic performance: 100% sensitivity, 91% specificity, 95% accuracy, and 100% precision compared to standard urine culture. 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SERS-based aptasensor for culture-free detection of Escherichia coli in urinary tract infection diagnosis
A surface-enhanced Raman scattering (SERS)-based aptasensor was developed for the rapid and sensitive detection of Escherichia coli (E. coli), a major pathogen in urinary tract infections (UTIs). The sensor utilizes magnetic beads embedded with gold nanoparticles (MB-AuNPs) functionalized with capture DNA (cDNA) as both the SERS-active substrate and magnetic separation tool. The detection mechanism relies on an aptamer DNA-probe DNA complex: when the aptamer binds specifically to E. coli, the probe DNA is released and subsequently hybridizes with cDNA on the MB-AuNPs. This brings a Cy5 Raman label close to the gold surface, generating a strong SERS signal. The assay offers a one-step process, eliminating the need for bacterial culture or nucleic acid amplification, and completes within approximately 6 h. Quantitative analysis demonstrated a detection limit of 5.9 × 103 CFU/mL, well below the clinical threshold for UTIs, with a reliable calibration curve (R2 = 0.990). Selectivity tests confirmed high specificity for E. coli without cross-reactivity to other bacteria. Clinical evaluation using 21 urine samples showed high diagnostic performance: 100% sensitivity, 91% specificity, 95% accuracy, and 100% precision compared to standard urine culture. These results highlight the aptasensor’s potential as a rapid, sensitive, and specific alternative for UTI diagnosis in clinical settings.
期刊介绍：
Nano Convergence is an internationally recognized, peer-reviewed, and interdisciplinary journal designed to foster effective communication among scientists spanning diverse research areas closely aligned with nanoscience and nanotechnology. Dedicated to encouraging the convergence of technologies across the nano- to microscopic scale, the journal aims to unveil novel scientific domains and cultivate fresh research prospects.
Operating on a single-blind peer-review system, Nano Convergence ensures transparency in the review process, with reviewers cognizant of authors' names and affiliations while maintaining anonymity in the feedback provided to authors.
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