美味猕猴桃衍生的外泌体样纳米颗粒抗真菌活性的评价。

IF 3.9 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Scientific Reports Pub Date : 2025-08-18 DOI:10.1038/s41598-025-13792-9

Dina Salem, Ahmed Z Abdel Aziz

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引用次数: 0

摘要

外泌体样纳米颗粒（ELNs）对于生物界内和界间的通信都是必不可少的。尽管植物eln具有重要的生物学意义，但其排放到环境中的存在和功能尚不清楚。本研究采用聚合物沉淀法从猕猴桃（Actinidia deliciosa）中分离出eln，对其进行鉴定，并从萌发率和孢子形成密度方面评价其对尖孢镰刀菌（f.s oxysporum）、番茄镰刀菌（f.s solani）和灰绿镰刀菌（b.s cinerea）的抑菌活性。根据动态光散射和透射电子显微镜的生物物理评估，发现根释放的EVs（细胞外囊泡）的大小在30至150 nm之间。利用LC-MS/MS鉴定了33种独特的蛋白。已知其中一些蛋白质与植物和微生物之间的相互作用有关。这些EVs中存在多种植物防御相关蛋白，这表明它们可能是植物免疫的重要组成部分。

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Assessment of the antifungal activity of Actinidia deliciosa derived exosome like nanoparticles.

The Exosome-like nanoparticles (ELNs) are essential for both intra- and inter-kingdom communication. The presence and function of plant ELNs discharged into the environment remain unknown, despite their significant biological significance. In the present study, polymer precipitation method was used to isolate the ELNs from Actinidia deliciosa followed by characterization and antifungal activity evaluation against F. oxysporum, F. solani, and B. cinerea in the term of germination and density of fungal spores' formation. Root-released EVs (extracellular vesicles) were found to have a size ranged from 30 to 150 nm, according to biophysical assessments using dynamic light scattering, and transmission electron microscope. Thirty-three unique proteins were identified using LC-MS/MS of ELNs. Several of these proteins are known to be associated with interactions between plants and microbes. The presence of multiple plant defense-related proteins in these EVs raises the possibility that they are important elements of plant immunity.

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来源期刊

Scientific Reports Natural Science Disciplines-

CiteScore

7.50

自引率

4.30%

发文量

19567

审稿时长

3.9 months

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