含1氧化还原酶样结构域对缺氧心肌细胞线粒体的保护作用。

IF 2.9 Q2 MEDICINE, RESEARCH & EXPERIMENTAL
Medical Gas Research Pub Date : 2026-06-01 Epub Date: 2025-08-18 DOI:10.4103/mgr.MEDGASRES-D-24-00117
Yan Yan, Min Dong, Liuyang Tian, Chao Zhu, Xiaojing Zhao
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引用次数: 0

摘要

尽管线粒体和相关蛋白对线粒体保存至关重要,但其中一些蛋白的功能尚不清楚。含有1的新型氧化还原酶样结构域(OXLD1/C17orf90, UniProtKB Q5BKU9)因其与线粒体的相关性而引起了人们的关注。本研究显示,在1%氧气环境下培养24小时的心肌细胞中,OXLD1水平下降。在常氧和缺氧条件下,抑制OXLD1都会增加线粒体损伤。线粒体膜电位下降和活性氧产生增加证明了这一点。同时,抑制OXLD1可降低线粒体氧化磷酸化。在常氧和缺氧条件下,过表达OXLD1可以减少线粒体损伤,这可以从线粒体膜电位增加和活性氧产生减少中看出。此外,过表达OXLD1增强了线粒体氧化磷酸化。此外,我们发现OXLD1通过影响线粒体复合物I和v来调节线粒体氧化磷酸化。在低氧条件下,OXLD1通过改善线粒体功能在保护心肌细胞中起着至关重要的作用。OXLD1通过与线粒体复合体I和v的相互作用来实现这种保护,因此,OXLD1可能是线粒体功能的一个新的重要调节因子。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mitochondrial protection role of oxidoreductase-like domain containing 1 in myocardial cells under hypoxia.

JOURNAL/mgres/04.03/01612956-202606000-00006/figure1/v/2025-08-18T154854Z/r/image-tiff Although mitochondria and related proteins are essential for mitochondrial preservation, the functions of some of these proteins remain unknown. The novel protein oxidoreductase-like domain containing 1 (OXLD1/C17orf90, UniProtKB Q5BKU9) have attracted our attention because of its correlation with mitochondria. This study revealed a decrease in OXLD1 levels in cardiomyocytes cultured in 1% oxygen for 24 hours. Suppressing OXLD1 increases mitochondrial injury under both normoxic and hypoxic conditions. This is evidenced by decreased mitochondrial membrane potential and increased reactive oxygen species production. Meanwhile, suppressing OXLD1 decreased mitochondrial oxidative phosphorylation. Overexpression of OXLD1 decreased mitochondrial injury under normoxia and hypoxia, as indicated by an increase in the mitochondrial membrane potential and a decrease in reactive oxygen species production. Moreover, overexpression of OXLD1 enhanced mitochondrial oxidative phosphorylation. Additionally, we found that OXLD1 regulates mitochondrial oxidative phosphorylation by affecting mitochondrial complexes I and V. OXLD1 plays a crucial role in protecting cardiomyocytes by improving mitochondrial function under low-oxygen conditions. OXLD1 achieves this protection through interactions with mitochondrial complexes I and V. Therefore, OXLD1 may serve as a new and important regulator of mitochondrial function.

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来源期刊
Medical Gas Research
Medical Gas Research MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
5.10
自引率
13.80%
发文量
35
期刊介绍: Medical Gas Research is an open access journal which publishes basic, translational, and clinical research focusing on the neurobiology as well as multidisciplinary aspects of medical gas research and their applications to related disorders. The journal covers all areas of medical gas research, but also has several special sections. Authors can submit directly to these sections, whose peer-review process is overseen by our distinguished Section Editors: Inert gases - Edited by Xuejun Sun and Mark Coburn, Gasotransmitters - Edited by Atsunori Nakao and John Calvert, Oxygen and diving medicine - Edited by Daniel Rossignol and Ke Jian Liu, Anesthetic gases - Edited by Richard Applegate and Zhongcong Xie, Medical gas in other fields of biology - Edited by John Zhang. Medical gas is a large family including oxygen, hydrogen, carbon monoxide, carbon dioxide, nitrogen, xenon, hydrogen sulfide, nitrous oxide, carbon disulfide, argon, helium and other noble gases. These medical gases are used in multiple fields of clinical practice and basic science research including anesthesiology, hyperbaric oxygen medicine, diving medicine, internal medicine, emergency medicine, surgery, and many basic sciences disciplines such as physiology, pharmacology, biochemistry, microbiology and neurosciences. Due to the unique nature of medical gas practice, Medical Gas Research will serve as an information platform for educational and technological advances in the field of medical gas.
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