大型动物三维成像组织光学清除研究进展。

IF 5.2 3区 工程技术 Q2 ENGINEERING, ELECTRICAL & ELECTRONIC
Yating Deng, Jianyi Xu, Tingting Yu, Dan Zhu
{"title":"大型动物三维成像组织光学清除研究进展。","authors":"Yating Deng, Jianyi Xu, Tingting Yu, Dan Zhu","doi":"10.1007/s12200-025-00162-6","DOIUrl":null,"url":null,"abstract":"<p><p>Three-dimensional reconstruction of tissue architecture is crucial for biomedical research. Tissue optical clearing technology overcomes light scattering limitations in biological tissues, providing an essential tool for high-resolution three-dimensional imaging. Given the high degree of similarity between large model animals (e.g., pigs, non-human primates) and humans in terms of anatomical structure, physiologic function, and disease mechanisms, the application of this technology in these models holds significant value for biomedical research. While well-established tissue clearing protocols exist for tissue sections, whole organs, and even entire bodies in rodents, scaling up to large animal specimens presents substantial challenges due to dimensional effects and compositional variations. This review systematically examines the methodological translation from rodent to large animals, particularly on species-specific differences in brain architecture and parenchymal organ composition that critically impact clearing efficiency. We comprehensively summarize recent applications in large animals, focusing on representative areas including neural circuit mapping, sensory organ imaging, and other related research domains, while proposing optimization strategies to overcome cross-species compatibility barriers. We hope this review will serve as a valuable reference for advancing tissue optical clearing applications in large-animal biomedical research.</p>","PeriodicalId":12685,"journal":{"name":"Frontiers of Optoelectronics","volume":"18 1","pages":"18"},"PeriodicalIF":5.2000,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12361029/pdf/","citationCount":"0","resultStr":"{\"title\":\"Advances in tissue optical clearing for 3D imaging in large animal.\",\"authors\":\"Yating Deng, Jianyi Xu, Tingting Yu, Dan Zhu\",\"doi\":\"10.1007/s12200-025-00162-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Three-dimensional reconstruction of tissue architecture is crucial for biomedical research. Tissue optical clearing technology overcomes light scattering limitations in biological tissues, providing an essential tool for high-resolution three-dimensional imaging. Given the high degree of similarity between large model animals (e.g., pigs, non-human primates) and humans in terms of anatomical structure, physiologic function, and disease mechanisms, the application of this technology in these models holds significant value for biomedical research. While well-established tissue clearing protocols exist for tissue sections, whole organs, and even entire bodies in rodents, scaling up to large animal specimens presents substantial challenges due to dimensional effects and compositional variations. This review systematically examines the methodological translation from rodent to large animals, particularly on species-specific differences in brain architecture and parenchymal organ composition that critically impact clearing efficiency. We comprehensively summarize recent applications in large animals, focusing on representative areas including neural circuit mapping, sensory organ imaging, and other related research domains, while proposing optimization strategies to overcome cross-species compatibility barriers. We hope this review will serve as a valuable reference for advancing tissue optical clearing applications in large-animal biomedical research.</p>\",\"PeriodicalId\":12685,\"journal\":{\"name\":\"Frontiers of Optoelectronics\",\"volume\":\"18 1\",\"pages\":\"18\"},\"PeriodicalIF\":5.2000,\"publicationDate\":\"2025-08-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12361029/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Frontiers of Optoelectronics\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1007/s12200-025-00162-6\",\"RegionNum\":3,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENGINEERING, ELECTRICAL & ELECTRONIC\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers of Optoelectronics","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s12200-025-00162-6","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}
引用次数: 0

摘要

组织结构的三维重建对生物医学研究至关重要。组织光学清除技术克服了生物组织中的光散射限制,为高分辨率三维成像提供了重要工具。鉴于大型模型动物(如猪、非人灵长类动物)与人类在解剖结构、生理功能和疾病机制方面高度相似,该技术在这些模型中的应用对生物医学研究具有重要价值。虽然在啮齿类动物的组织切片、整个器官甚至整个身体中存在完善的组织清除方案,但由于尺寸效应和成分变化,扩大到大型动物标本存在重大挑战。这篇综述系统地研究了从啮齿动物到大型动物的方法转换,特别是大脑结构和实质器官组成的物种特异性差异,这些差异对清除效率有重要影响。本文综合总结了近年来在大型动物研究中的应用,重点介绍了神经回路映射、感觉器官成像等具有代表性的研究领域,并提出了克服跨物种相容性障碍的优化策略。希望本文的研究能为推进组织光学清除在大型动物生物医学研究中的应用提供有价值的参考。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Advances in tissue optical clearing for 3D imaging in large animal.

Three-dimensional reconstruction of tissue architecture is crucial for biomedical research. Tissue optical clearing technology overcomes light scattering limitations in biological tissues, providing an essential tool for high-resolution three-dimensional imaging. Given the high degree of similarity between large model animals (e.g., pigs, non-human primates) and humans in terms of anatomical structure, physiologic function, and disease mechanisms, the application of this technology in these models holds significant value for biomedical research. While well-established tissue clearing protocols exist for tissue sections, whole organs, and even entire bodies in rodents, scaling up to large animal specimens presents substantial challenges due to dimensional effects and compositional variations. This review systematically examines the methodological translation from rodent to large animals, particularly on species-specific differences in brain architecture and parenchymal organ composition that critically impact clearing efficiency. We comprehensively summarize recent applications in large animals, focusing on representative areas including neural circuit mapping, sensory organ imaging, and other related research domains, while proposing optimization strategies to overcome cross-species compatibility barriers. We hope this review will serve as a valuable reference for advancing tissue optical clearing applications in large-animal biomedical research.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Frontiers of Optoelectronics
Frontiers of Optoelectronics ENGINEERING, ELECTRICAL & ELECTRONIC-
CiteScore
7.80
自引率
0.00%
发文量
583
期刊介绍: Frontiers of Optoelectronics seeks to provide a multidisciplinary forum for a broad mix of peer-reviewed academic papers in order to promote rapid communication and exchange between researchers in China and abroad. It introduces and reflects significant achievements being made in the field of photonics or optoelectronics. The topics include, but are not limited to, semiconductor optoelectronics, nano-photonics, information photonics, energy photonics, ultrafast photonics, biomedical photonics, nonlinear photonics, fiber optics, laser and terahertz technology and intelligent photonics. The journal publishes reviews, research articles, letters, comments, special issues and so on. Frontiers of Optoelectronics especially encourages papers from new emerging and multidisciplinary areas, papers reflecting the international trends of research and development, and on special topics reporting progress made in the field of optoelectronics. All published papers will reflect the original thoughts of researchers and practitioners on basic theories, design and new technology in optoelectronics. Frontiers of Optoelectronics is strictly peer-reviewed and only accepts original submissions in English. It is a fully OA journal and the APCs are covered by Higher Education Press and Huazhong University of Science and Technology. ● Presents the latest developments in optoelectronics and optics ● Emphasizes the latest developments of new optoelectronic materials, devices, systems and applications ● Covers industrial photonics, information photonics, biomedical photonics, energy photonics, laser and terahertz technology, and more
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信