决明子瘘花制备的聚乙二醇偶联硒纳米复合材料对HepG2细胞凋亡和细胞周期阻滞的体外评估。

IF 2.3 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BioMed Research International Pub Date : 2025-08-09 eCollection Date: 2025-01-01 DOI:10.1155/bmri/6212199
Senthamaraikannan Yasodha, Sundaram Vickram, Shanmugam Rajeshkumar, Hitesh Chopra, Tabarak Malik
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引用次数: 0

摘要

聚合物包封纳米复合材料已被证明在治疗癌症方面具有改善作用。本研究的主要目的是制备聚乙二醇(PEG)基决明子瘘花硒纳米复合材料(CFF-SeNC)。利用紫外可见分光光度计、傅里叶变换红外光谱(FTIR)、扫描电子显微镜(SEM)-能量色散x射线(EDX)和x射线衍射仪(XRD)对制备的CFF-SeNC进行了表征。紫外可见吸收光谱在355 nm处最高。SEM结果显示,纳米复合材料呈椭圆形,表面粗糙,团聚较少,元素组成为碳、氧和硒。XRD分析结果显示了CFF-SeNC的晶型。CFF-SeNC对病原菌和真菌的抑菌效果证明了CFF-SeNC对白色念珠菌的抑菌效果。在最大浓度(50 μg/mL)下,cff - senc对dpph、ABTS和H2O2的抑制率分别为85.51%±0.79%、90.53%±0.90%和84.87%±0.80%。鸡蛋白蛋白变性和人红细胞膜稳定性实验表明,CFF-SeNC的抗炎效率分别为80.61%±0.87%和87.78%±0.88%。用盐水对虾致死活性测试了CFF-SeNC的生物相容性,结果表明,在48h时,最小剂量没有毒性;然而,在较高的剂量下显示出轻微的毒性。在27.30 μg/mL浓度下,CFF-SeNC对HepG2细胞有50%的抑制作用(IC50值)。HepG2细胞在CFF-SeNC处理后表现出明显的形态学变化,而Vero细胞则没有变化。通过荧光显微镜、细胞周期阻滞和流式细胞术Annexin V/碘化丙啶染色进一步分析细胞凋亡的分期。目前的研究结果证实了聚乙二醇包封硒纳米复合材料的治疗效果,并强调了其对肝癌(HepG2细胞)的凋亡作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In Vitro Assessment of Apoptotic and Cell Cycle Arrest Analysis on HepG2 Cells by Polyethylene Glycol-Coupled Selenium Nanocomposite Fabricated From Cassia fistula Flowers.

Polymer-encapsulated nanocomposite has been proven to have ameliorative effects in the treatment of cancer. The focused objective of the present research is to fabricate polyethylene glycol (PEG)-based Cassia fistula flower selenium nanocomposite (CFF-SeNC). The fabricated CFF-SeNC was characterised using a UV-visible spectrophotometer, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM)-energy dispersive x-ray (EDX), and x-ray diffractometer (XRD) analysis. The highest UV-visible absorption spectra were at 355 nm. The oval-shaped, less agglomerated nanocomposite with a rough surface was seen in the SEM results with the elemental composition of carbon, oxygen, and selenium. The XRD analysis results showed the crystalline form of CFF-SeNC. The potency of CFF-SeNC against pathogenic bacteria and fungi proved that CFF-SeNC is effective against the tested fungi Candida albicans. The antioxidative assays-DPPH, ABTS, and H2O2 of CFF-SeNC-revealed the inhibition percent of 85.51% ± 0.79%, 90.53% ± 0.90%, and 84.87% ± 0.80% at maximum concentration (50 μg/mL), respectively. The anti-inflammatory efficiency of CFF-SeNC was found to be 80.61% ± 0.87% and 87.78% ± 0.88% by egg albumin denaturation and HRBCs (human red blood cells) membrane stabilisation assay. The biocompatible nature of CFF-SeNC was tested by brine shrimp lethality activity, which proved that at 48 h, the minimal dose was not toxic; however, mild toxicity was shown at a higher dosage. The anticancer studies of CFF-SeNC in HepG2 cells were active in inhibiting 50% of cells (IC50 value) at 27.30 μg/mL. HepG2 cells depicted predominant morphological changes upon treatment with CFF-SeNC, whereas there was no alteration in Vero cells. The elaborative study on determining the phases of cell apoptosis was further analysed by fluorescence microscopy, cell cycle arrest, and Annexin V/propidium iodide staining through flow cytometric analysis. The outcome of current research has corroborated the enhanced therapeutical efficiency of PEG-encapsulated selenium nanocomposite with emphasis on the apoptotic effect in hepatocellular carcinoma (HepG2 cells).

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来源期刊
BioMed Research International
BioMed Research International BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MEDICINE, RESEARCH & EXPERIMENTAL
CiteScore
6.70
自引率
0.00%
发文量
1942
审稿时长
19 weeks
期刊介绍: BioMed Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies covering a wide range of subjects in life sciences and medicine. The journal is divided into 55 subject areas.
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