宽带1ghz双频梳的中红外高光谱显微镜。

IF 5.3 1区 物理与天体物理 Q1 OPTICS
APL Photonics Pub Date : 2024-10-01 DOI:10.1063/5.0225616
Peter Chang, Ragib Ishrak, Nazanin Hoghooghi, Scott Egbert, Daniel Lesko, Stephanie Swartz, Jens Biegert, Gregory B Rieker, Rohith Reddy, Scott A Diddams
{"title":"宽带1ghz双频梳的中红外高光谱显微镜。","authors":"Peter Chang, Ragib Ishrak, Nazanin Hoghooghi, Scott Egbert, Daniel Lesko, Stephanie Swartz, Jens Biegert, Gregory B Rieker, Rohith Reddy, Scott A Diddams","doi":"10.1063/5.0225616","DOIUrl":null,"url":null,"abstract":"<p><p>Mid-infrared microscopy is an important tool for biological analyses, allowing a direct probe of molecular bonds in their low energy landscape. In addition to the label-free extraction of spectroscopic information, the application of broadband sources can provide a third dimension of chemical specificity. However, to enable widespread deployment, mid-infrared microscopy platforms need to be compact and robust while offering high speed, broad bandwidth, and high signal-to-noise ratio. In this study, we experimentally showcase the integration of a broadband, high-repetition-rate dual-comb spectrometer (DCS) in the mid-infrared range with a scanning microscope. We employ a set of 1-GHz mid-infrared frequency combs, demonstrating their capability for high-speed and broadband hyperspectral imaging of polymers and ovarian tissue. The system covers <math><mn>1000</mn> <mspace></mspace> <msup><mrow><mtext>cm</mtext></mrow> <mrow><mo>-</mo> <mn>1</mn></mrow> </msup> </math> at <math> <msub><mrow><mi>v</mi></mrow> <mrow><mtext>c</mtext></mrow> </msub> <mo>=</mo> <mn>2941</mn> <mspace></mspace> <msup><mrow><mtext>cm</mtext></mrow> <mrow><mo>-</mo> <mn>1</mn></mrow> </msup> </math> with 12.86 kHz spectra acquisition rate and <math><mn>5</mn> <mspace></mspace> <mi>μ</mi> <mtext>m</mtext></math> spatial resolution. Taken together, our experiments and analysis elucidate the trade-off between bandwidth and speed in DCS as it relates to microscopy. This provides a roadmap for the future advancement and application of high-repetition-rate DCS hyperspectral imaging.</p>","PeriodicalId":8198,"journal":{"name":"APL Photonics","volume":"9 10","pages":""},"PeriodicalIF":5.3000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12352579/pdf/","citationCount":"0","resultStr":"{\"title\":\"Mid-infrared hyperspectral microscopy with broadband 1-GHz dual frequency combs.\",\"authors\":\"Peter Chang, Ragib Ishrak, Nazanin Hoghooghi, Scott Egbert, Daniel Lesko, Stephanie Swartz, Jens Biegert, Gregory B Rieker, Rohith Reddy, Scott A Diddams\",\"doi\":\"10.1063/5.0225616\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Mid-infrared microscopy is an important tool for biological analyses, allowing a direct probe of molecular bonds in their low energy landscape. In addition to the label-free extraction of spectroscopic information, the application of broadband sources can provide a third dimension of chemical specificity. However, to enable widespread deployment, mid-infrared microscopy platforms need to be compact and robust while offering high speed, broad bandwidth, and high signal-to-noise ratio. In this study, we experimentally showcase the integration of a broadband, high-repetition-rate dual-comb spectrometer (DCS) in the mid-infrared range with a scanning microscope. We employ a set of 1-GHz mid-infrared frequency combs, demonstrating their capability for high-speed and broadband hyperspectral imaging of polymers and ovarian tissue. The system covers <math><mn>1000</mn> <mspace></mspace> <msup><mrow><mtext>cm</mtext></mrow> <mrow><mo>-</mo> <mn>1</mn></mrow> </msup> </math> at <math> <msub><mrow><mi>v</mi></mrow> <mrow><mtext>c</mtext></mrow> </msub> <mo>=</mo> <mn>2941</mn> <mspace></mspace> <msup><mrow><mtext>cm</mtext></mrow> <mrow><mo>-</mo> <mn>1</mn></mrow> </msup> </math> with 12.86 kHz spectra acquisition rate and <math><mn>5</mn> <mspace></mspace> <mi>μ</mi> <mtext>m</mtext></math> spatial resolution. Taken together, our experiments and analysis elucidate the trade-off between bandwidth and speed in DCS as it relates to microscopy. This provides a roadmap for the future advancement and application of high-repetition-rate DCS hyperspectral imaging.</p>\",\"PeriodicalId\":8198,\"journal\":{\"name\":\"APL Photonics\",\"volume\":\"9 10\",\"pages\":\"\"},\"PeriodicalIF\":5.3000,\"publicationDate\":\"2024-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12352579/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"APL Photonics\",\"FirstCategoryId\":\"101\",\"ListUrlMain\":\"https://doi.org/10.1063/5.0225616\",\"RegionNum\":1,\"RegionCategory\":\"物理与天体物理\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"OPTICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"APL Photonics","FirstCategoryId":"101","ListUrlMain":"https://doi.org/10.1063/5.0225616","RegionNum":1,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"OPTICS","Score":null,"Total":0}
引用次数: 0

摘要

中红外显微镜是一种重要的生物分析工具,可以直接探测分子键在其低能景观。除了光谱信息的无标签提取外,宽带源的应用还可以提供第三维度的化学特异性。然而,为了实现广泛的部署,中红外显微镜平台需要紧凑和强大,同时提供高速,宽带宽和高信噪比。在这项研究中,我们通过实验展示了中红外波段宽带、高重复率双梳光谱仪(DCS)与扫描显微镜的集成。我们使用了一组1 ghz的中红外频率梳子,展示了它们对聚合物和卵巢组织进行高速和宽带高光谱成像的能力。该系统覆盖范围为1000cm - 1, vc = 2941 cm - 1,光谱采集率为12.86 kHz,空间分辨率为5 μ m。综上所述,我们的实验和分析阐明了DCS中带宽和速度之间的权衡,因为它与显微镜有关。这为高重复率DCS高光谱成像的未来发展和应用提供了路线图。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mid-infrared hyperspectral microscopy with broadband 1-GHz dual frequency combs.

Mid-infrared microscopy is an important tool for biological analyses, allowing a direct probe of molecular bonds in their low energy landscape. In addition to the label-free extraction of spectroscopic information, the application of broadband sources can provide a third dimension of chemical specificity. However, to enable widespread deployment, mid-infrared microscopy platforms need to be compact and robust while offering high speed, broad bandwidth, and high signal-to-noise ratio. In this study, we experimentally showcase the integration of a broadband, high-repetition-rate dual-comb spectrometer (DCS) in the mid-infrared range with a scanning microscope. We employ a set of 1-GHz mid-infrared frequency combs, demonstrating their capability for high-speed and broadband hyperspectral imaging of polymers and ovarian tissue. The system covers 1000 cm - 1 at v c = 2941 cm - 1 with 12.86 kHz spectra acquisition rate and 5 μ m spatial resolution. Taken together, our experiments and analysis elucidate the trade-off between bandwidth and speed in DCS as it relates to microscopy. This provides a roadmap for the future advancement and application of high-repetition-rate DCS hyperspectral imaging.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
APL Photonics
APL Photonics Physics and Astronomy-Atomic and Molecular Physics, and Optics
CiteScore
10.30
自引率
3.60%
发文量
107
审稿时长
19 weeks
期刊介绍: APL Photonics is the new dedicated home for open access multidisciplinary research from and for the photonics community. The journal publishes fundamental and applied results that significantly advance the knowledge in photonics across physics, chemistry, biology and materials science.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信