Peter Chang, Ragib Ishrak, Nazanin Hoghooghi, Scott Egbert, Daniel Lesko, Stephanie Swartz, Jens Biegert, Gregory B Rieker, Rohith Reddy, Scott A Diddams
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We employ a set of 1-GHz mid-infrared frequency combs, demonstrating their capability for high-speed and broadband hyperspectral imaging of polymers and ovarian tissue. The system covers <math><mn>1000</mn> <mspace></mspace> <msup><mrow><mtext>cm</mtext></mrow> <mrow><mo>-</mo> <mn>1</mn></mrow> </msup> </math> at <math> <msub><mrow><mi>v</mi></mrow> <mrow><mtext>c</mtext></mrow> </msub> <mo>=</mo> <mn>2941</mn> <mspace></mspace> <msup><mrow><mtext>cm</mtext></mrow> <mrow><mo>-</mo> <mn>1</mn></mrow> </msup> </math> with 12.86 kHz spectra acquisition rate and <math><mn>5</mn> <mspace></mspace> <mi>μ</mi> <mtext>m</mtext></math> spatial resolution. Taken together, our experiments and analysis elucidate the trade-off between bandwidth and speed in DCS as it relates to microscopy. 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The system covers <math><mn>1000</mn> <mspace></mspace> <msup><mrow><mtext>cm</mtext></mrow> <mrow><mo>-</mo> <mn>1</mn></mrow> </msup> </math> at <math> <msub><mrow><mi>v</mi></mrow> <mrow><mtext>c</mtext></mrow> </msub> <mo>=</mo> <mn>2941</mn> <mspace></mspace> <msup><mrow><mtext>cm</mtext></mrow> <mrow><mo>-</mo> <mn>1</mn></mrow> </msup> </math> with 12.86 kHz spectra acquisition rate and <math><mn>5</mn> <mspace></mspace> <mi>μ</mi> <mtext>m</mtext></math> spatial resolution. Taken together, our experiments and analysis elucidate the trade-off between bandwidth and speed in DCS as it relates to microscopy. 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Mid-infrared hyperspectral microscopy with broadband 1-GHz dual frequency combs.
Mid-infrared microscopy is an important tool for biological analyses, allowing a direct probe of molecular bonds in their low energy landscape. In addition to the label-free extraction of spectroscopic information, the application of broadband sources can provide a third dimension of chemical specificity. However, to enable widespread deployment, mid-infrared microscopy platforms need to be compact and robust while offering high speed, broad bandwidth, and high signal-to-noise ratio. In this study, we experimentally showcase the integration of a broadband, high-repetition-rate dual-comb spectrometer (DCS) in the mid-infrared range with a scanning microscope. We employ a set of 1-GHz mid-infrared frequency combs, demonstrating their capability for high-speed and broadband hyperspectral imaging of polymers and ovarian tissue. The system covers at with 12.86 kHz spectra acquisition rate and spatial resolution. Taken together, our experiments and analysis elucidate the trade-off between bandwidth and speed in DCS as it relates to microscopy. This provides a roadmap for the future advancement and application of high-repetition-rate DCS hyperspectral imaging.
APL PhotonicsPhysics and Astronomy-Atomic and Molecular Physics, and Optics
CiteScore
10.30
自引率
3.60%
发文量
107
审稿时长
19 weeks
期刊介绍:
APL Photonics is the new dedicated home for open access multidisciplinary research from and for the photonics community. The journal publishes fundamental and applied results that significantly advance the knowledge in photonics across physics, chemistry, biology and materials science.