Joana Zorzal Nodari, Felipe Donateli Gatti, Thiago de Jesus Sousa, Gabriela Colombo de Mendonça, Thabata Coeli Dias Damasceno, Sarah Martins Presti da Silva, Eric Arrivabene Tavares, Suwellen Sardinha Dias de Azevedo, Jaqueline Pegoretti Goulart, Edson Delatorre, Rodrigo Ribeiro-Rodrigues
{"title":"虫媒病毒合并感染的基因组学和流行病学调查，Espírito Santo，巴西。","authors":"Joana Zorzal Nodari, Felipe Donateli Gatti, Thiago de Jesus Sousa, Gabriela Colombo de Mendonça, Thabata Coeli Dias Damasceno, Sarah Martins Presti da Silva, Eric Arrivabene Tavares, Suwellen Sardinha Dias de Azevedo, Jaqueline Pegoretti Goulart, Edson Delatorre, Rodrigo Ribeiro-Rodrigues","doi":"10.1016/j.ajic.2025.08.008","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>In Brazil, the simultaneous circulation of arboviruses is driven by anthropological factors. In places with intense arbovirus transmission, such as Espírito Santo, it is important to investigate the epidemiology of coinfections in order to provide information to improve surveillance and management strategies.</p><p><strong>Methods: </strong>We assessed their prevalence, clinical impact, and viral diversity in Espírito Santo, Brazil (2023-2024), testing 66,019 patients for dengue, chikungunya, Zika, Mayaro, and Oropouche viruses via RT-qPCR and genomic sequencing.</p><p><strong>Results: </strong>Overall, 34.45% tested positive, mainly for OROV (21.93%), DENV (19.57%), and CHIKV (6.94%), while ZIKV and MAYV were undetected. Coinfections were rare (0.2%, n = 44), mostly DENV/CHIKV (75%), with 2 novel DENV-1/DENV-2/CHIKV cases and 1 OROV/DENV-2. Sequencing yielded 12 complete viral genomes, identifying distinct DENV-1, DENV-2, CHIKV, and OROV lineages.</p><p><strong>Discussion: </strong>Genomic characterization of coinfection samples provided a comprehensive view of arboviral genetic diversity, revealing the presence of multiple cocirculating strains. Despite the hyperendemic scenario, coinfections were rare, probably due to complex interactions between viral interference, host immunity, vector behavior and diagnostic challenges.</p><p><strong>Conclusions: </strong>We report the first triple arbovirus infection documented in Espírito Santo and coinfection with OROV outside endemic areas, emphasizing the importance of molecular diagnosis and ongoing research to understand arboviral dynamics and prepare for outbreaks.</p>","PeriodicalId":7621,"journal":{"name":"American journal of infection control","volume":" ","pages":""},"PeriodicalIF":2.4000,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genomic and epidemiologic investigation of arboviral coinfections, Espírito Santo, Brazil.\",\"authors\":\"Joana Zorzal Nodari, Felipe Donateli Gatti, Thiago de Jesus Sousa, Gabriela Colombo de Mendonça, Thabata Coeli Dias Damasceno, Sarah Martins Presti da Silva, Eric Arrivabene Tavares, Suwellen Sardinha Dias de Azevedo, Jaqueline Pegoretti Goulart, Edson Delatorre, Rodrigo Ribeiro-Rodrigues\",\"doi\":\"10.1016/j.ajic.2025.08.008\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>In Brazil, the simultaneous circulation of arboviruses is driven by anthropological factors. In places with intense arbovirus transmission, such as Espírito Santo, it is important to investigate the epidemiology of coinfections in order to provide information to improve surveillance and management strategies.</p><p><strong>Methods: </strong>We assessed their prevalence, clinical impact, and viral diversity in Espírito Santo, Brazil (2023-2024), testing 66,019 patients for dengue, chikungunya, Zika, Mayaro, and Oropouche viruses via RT-qPCR and genomic sequencing.</p><p><strong>Results: </strong>Overall, 34.45% tested positive, mainly for OROV (21.93%), DENV (19.57%), and CHIKV (6.94%), while ZIKV and MAYV were undetected. Coinfections were rare (0.2%, n = 44), mostly DENV/CHIKV (75%), with 2 novel DENV-1/DENV-2/CHIKV cases and 1 OROV/DENV-2. Sequencing yielded 12 complete viral genomes, identifying distinct DENV-1, DENV-2, CHIKV, and OROV lineages.</p><p><strong>Discussion: </strong>Genomic characterization of coinfection samples provided a comprehensive view of arboviral genetic diversity, revealing the presence of multiple cocirculating strains. Despite the hyperendemic scenario, coinfections were rare, probably due to complex interactions between viral interference, host immunity, vector behavior and diagnostic challenges.</p><p><strong>Conclusions: </strong>We report the first triple arbovirus infection documented in Espírito Santo and coinfection with OROV outside endemic areas, emphasizing the importance of molecular diagnosis and ongoing research to understand arboviral dynamics and prepare for outbreaks.</p>\",\"PeriodicalId\":7621,\"journal\":{\"name\":\"American journal of infection control\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2025-08-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"American journal of infection control\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1016/j.ajic.2025.08.008\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"American journal of infection control","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.ajic.2025.08.008","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Genomic and epidemiologic investigation of arboviral coinfections, Espírito Santo, Brazil.
Background: In Brazil, the simultaneous circulation of arboviruses is driven by anthropological factors. In places with intense arbovirus transmission, such as Espírito Santo, it is important to investigate the epidemiology of coinfections in order to provide information to improve surveillance and management strategies.
Methods: We assessed their prevalence, clinical impact, and viral diversity in Espírito Santo, Brazil (2023-2024), testing 66,019 patients for dengue, chikungunya, Zika, Mayaro, and Oropouche viruses via RT-qPCR and genomic sequencing.
Results: Overall, 34.45% tested positive, mainly for OROV (21.93%), DENV (19.57%), and CHIKV (6.94%), while ZIKV and MAYV were undetected. Coinfections were rare (0.2%, n = 44), mostly DENV/CHIKV (75%), with 2 novel DENV-1/DENV-2/CHIKV cases and 1 OROV/DENV-2. Sequencing yielded 12 complete viral genomes, identifying distinct DENV-1, DENV-2, CHIKV, and OROV lineages.
Discussion: Genomic characterization of coinfection samples provided a comprehensive view of arboviral genetic diversity, revealing the presence of multiple cocirculating strains. Despite the hyperendemic scenario, coinfections were rare, probably due to complex interactions between viral interference, host immunity, vector behavior and diagnostic challenges.
Conclusions: We report the first triple arbovirus infection documented in Espírito Santo and coinfection with OROV outside endemic areas, emphasizing the importance of molecular diagnosis and ongoing research to understand arboviral dynamics and prepare for outbreaks.
期刊介绍：
AJIC covers key topics and issues in infection control and epidemiology. Infection control professionals, including physicians, nurses, and epidemiologists, rely on AJIC for peer-reviewed articles covering clinical topics as well as original research. As the official publication of the Association for Professionals in Infection Control and Epidemiology (APIC)
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