{"title":"Zn单原子位/Ru纳米团簇协同诱导活性氧风暴用于生物标志物PSA的超灵敏电化学发光检测","authors":"Jia-Xi Sun, Yuan-Yuan Chen, Ai-Jun Wang, Lin-Mei Li, Pei-Xin Yuan, Jiu-Ju Feng","doi":"10.1007/s00604-025-07458-6","DOIUrl":null,"url":null,"abstract":"<div><p>Improving the sensitivity of electrochemiluminescence (ECL) systems is crucial for bio-marker detection and prostate cancer diagnosis, as early-stage prostate cancer exhibits low biomarkers levels. In this work, satellite Zn single-atom sites/Ru nanoclusters anchored on N-rich porous carbon (Zn SAs/Ru NCs) was synthesized by high-temperature pyrolysis under N<sub>2</sub> atmosphere. The Zn SAs/Ru NCs synergistically induced reactive oxygen species (ROS) storm andboosted luminol signals in the absence of H<sub>2</sub>O<sub>2</sub>, eliminating the effect of unstable H<sub>2</sub>O<sub>2</sub>. Subsequently, an ultrasensitive ECL biosensor was constructed for prostate-specific antigen (PSA) through specific immuno-recognition. The ECL biosensor exhibited a wide linear range from 0.1 pg·mL<sup>−1</sup> to 1000 ng·mL<sup>−1</sup> and a low detection limit of 1.072 fg·mL<sup>−1</sup>, enabling stable and ultra-sensitive PSA detection. Furthermore, the biosensor was applied to real serum samples, demonstrating its feasibility for clinical assays. This work provided a promising tool for early diagnosis and therapeutic evaluation of prostate cancer.\n</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 9","pages":""},"PeriodicalIF":5.3000,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Zn single-atom sites/Ru nanoclusters synergistically inducing reactive oxygen species storm for ultrasensitive electrochemiluminescence detection of biomarker PSA\",\"authors\":\"Jia-Xi Sun, Yuan-Yuan Chen, Ai-Jun Wang, Lin-Mei Li, Pei-Xin Yuan, Jiu-Ju Feng\",\"doi\":\"10.1007/s00604-025-07458-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Improving the sensitivity of electrochemiluminescence (ECL) systems is crucial for bio-marker detection and prostate cancer diagnosis, as early-stage prostate cancer exhibits low biomarkers levels. In this work, satellite Zn single-atom sites/Ru nanoclusters anchored on N-rich porous carbon (Zn SAs/Ru NCs) was synthesized by high-temperature pyrolysis under N<sub>2</sub> atmosphere. The Zn SAs/Ru NCs synergistically induced reactive oxygen species (ROS) storm andboosted luminol signals in the absence of H<sub>2</sub>O<sub>2</sub>, eliminating the effect of unstable H<sub>2</sub>O<sub>2</sub>. Subsequently, an ultrasensitive ECL biosensor was constructed for prostate-specific antigen (PSA) through specific immuno-recognition. The ECL biosensor exhibited a wide linear range from 0.1 pg·mL<sup>−1</sup> to 1000 ng·mL<sup>−1</sup> and a low detection limit of 1.072 fg·mL<sup>−1</sup>, enabling stable and ultra-sensitive PSA detection. Furthermore, the biosensor was applied to real serum samples, demonstrating its feasibility for clinical assays. This work provided a promising tool for early diagnosis and therapeutic evaluation of prostate cancer.\\n</p><h3>Graphical Abstract</h3>\\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":705,\"journal\":{\"name\":\"Microchimica Acta\",\"volume\":\"192 9\",\"pages\":\"\"},\"PeriodicalIF\":5.3000,\"publicationDate\":\"2025-08-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microchimica Acta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00604-025-07458-6\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchimica Acta","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00604-025-07458-6","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Zn single-atom sites/Ru nanoclusters synergistically inducing reactive oxygen species storm for ultrasensitive electrochemiluminescence detection of biomarker PSA
Improving the sensitivity of electrochemiluminescence (ECL) systems is crucial for bio-marker detection and prostate cancer diagnosis, as early-stage prostate cancer exhibits low biomarkers levels. In this work, satellite Zn single-atom sites/Ru nanoclusters anchored on N-rich porous carbon (Zn SAs/Ru NCs) was synthesized by high-temperature pyrolysis under N2 atmosphere. The Zn SAs/Ru NCs synergistically induced reactive oxygen species (ROS) storm andboosted luminol signals in the absence of H2O2, eliminating the effect of unstable H2O2. Subsequently, an ultrasensitive ECL biosensor was constructed for prostate-specific antigen (PSA) through specific immuno-recognition. The ECL biosensor exhibited a wide linear range from 0.1 pg·mL−1 to 1000 ng·mL−1 and a low detection limit of 1.072 fg·mL−1, enabling stable and ultra-sensitive PSA detection. Furthermore, the biosensor was applied to real serum samples, demonstrating its feasibility for clinical assays. This work provided a promising tool for early diagnosis and therapeutic evaluation of prostate cancer.
期刊介绍：
As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.
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