不同剂量枸杞多糖影响果蝇精子活力的机制研究

IF 2.8 4区 医学 Q2 REPRODUCTIVE BIOLOGY
Jingjing Yu , Wenjun Fu , Xinyi Wang , Xiang Zhang , Kun Xiong , Yuan Wang
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引用次数: 0

摘要

枸杞,一种茄科植物,在其干燥成熟的果实中产生生物活性多糖(LBP)。LBP已被证明对生殖系统有保护作用。本研究以黑腹果蝇为实验对象,探讨LBP对雄性生殖能力的剂量依赖性。采用CAFE法口服LBP(0 ~ 2000 mg/L)。24小时后,检测精子活力、排卵率和睾丸基因表达。关键结果:LBP以剂量依赖性的方式调节精子活力和女性排卵率。oamb、gish、zeste和cg9465的剂量响应曲线呈倒u型趋势。LBP通过OAMB受体调控生精基因。PKA信号与组蛋白甲基转移酶(zeste)呈正相关,表明存在其他调控途径。观察到两期剂量效应:LBP在0-150 mg/kg和450-1000 mg/kg时增强运动,而在150-450 mg/kg和>; 1000 mg/kg时抑制运动。综上所述,LBP的繁殖效应遵循非线性动态规律。这种复杂性进一步强调了对LBP各成分进行深入研究的必要性,以便准确评估其生殖毒性并优化其治疗潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Study on the mechanism of different doses of lycium barbarum polysaccharides affecting sperm motility in Drosophila melanogaster
Lycium barbarum, a Solanaceae plant, yields bioactive polysaccharides (LBP) in its dried mature fruits. LBP has demonstrated protective effects on the reproductive system. This study took Drosophila melanogaster as the experimental subject to investigate the dose-dependence of LBP on male reproductive capacity. The flies were orally administered LBP (0–2000 mg/L) by the CAFE method. Twenty-four hours later, sperm motility, ovulation rate and testicular gene expression were evaluated. Key results: LBP modulates sperm motility and female ovulation rate in a dose-dependent manner. The dose-response curves of oamb, gish, zeste and cg9465 show an inverted U-shaped trend. LBP regulates spermatogenic genes through OAMB receptor. The PKA signal was positively correlated with histone methyltransferase (zeste), indicating that there are other regulatory pathways. Two-phase dose effects were observed: LBP enhanced motility at 0–150 mg/kg and 450–1000 mg/kg, while inhibited motility at 150–450 mg/kg and > 1000 mg/kg. In conclusion, the reproductive effects of LBP follow nonlinear dynamic laws. This complexity further highlights the necessity of conducting in-depth studies on each component of LBP in order to accurately assess its reproductive toxicity and optimize its therapeutic potential.
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来源期刊
Reproductive toxicology
Reproductive toxicology 生物-毒理学
CiteScore
6.50
自引率
3.00%
发文量
131
审稿时长
45 days
期刊介绍: Drawing from a large number of disciplines, Reproductive Toxicology publishes timely, original research on the influence of chemical and physical agents on reproduction. Written by and for obstetricians, pediatricians, embryologists, teratologists, geneticists, toxicologists, andrologists, and others interested in detecting potential reproductive hazards, the journal is a forum for communication among researchers and practitioners. Articles focus on the application of in vitro, animal and clinical research to the practice of clinical medicine. All aspects of reproduction are within the scope of Reproductive Toxicology, including the formation and maturation of male and female gametes, sexual function, the events surrounding the fusion of gametes and the development of the fertilized ovum, nourishment and transport of the conceptus within the genital tract, implantation, embryogenesis, intrauterine growth, placentation and placental function, parturition, lactation and neonatal survival. Adverse reproductive effects in males will be considered as significant as adverse effects occurring in females. To provide a balanced presentation of approaches, equal emphasis will be given to clinical and animal or in vitro work. Typical end points that will be studied by contributors include infertility, sexual dysfunction, spontaneous abortion, malformations, abnormal histogenesis, stillbirth, intrauterine growth retardation, prematurity, behavioral abnormalities, and perinatal mortality.
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