Yue Lou, Longping Liu, Yuping Liu, Zheng Zhou, Lijing Zhu, Yajuan Cui, Huaxiu Sun, Xuan Zhou, Chuanxiang Zhou, Tiejun Li
{"title":"涎腺样囊性癌细胞外囊泡抑制肺免疫微环境。","authors":"Yue Lou, Longping Liu, Yuping Liu, Zheng Zhou, Lijing Zhu, Yajuan Cui, Huaxiu Sun, Xuan Zhou, Chuanxiang Zhou, Tiejun Li","doi":"10.1111/odi.70065","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Salivary adenoid cystic carcinoma (SACC) frequently metastasizes to the lung and affects patient survival. Extracellular vesicles (EVs) have significant effects in the tumor pre-metastatic niche (PMN). In this study, we aimed to investigate immune changes in the lung pre-metastatic microenvironment of SACC.</p><p><strong>Methods: </strong>A pre-metastatic microenvironment was established using SACC-derived EVs at different time points. Labeled EVs were injected into mice to examine their different organ distributions. Flow cytometry was conducted to analyze the immune cell populations. In vitro, the same amount of SACC-derived EVs or EV-depleted conditional medium was added to macrophages to stimulate polarization.</p><p><strong>Results: </strong>The SACC-derived EVs colonized mostly in the lung and were engulfed by lung interstitial macrophages (IM). At different time points, the SACC-derived EVs induced decreased total macrophage, natural killer cell, and T cell populations, especially CD8<sup>+</sup> T cells. They also increased the M2 macrophage composition and regulatory T cell population. The in vitro study showed that SACC-derived EVs induce a mixed M1/M2 phenotype, but this effect was eliminated with the EV-depleted media.</p><p><strong>Conclusions: </strong>SACC-derived EVs circulate to the lung, where they are engulfed by IM and induce an immunosuppressive environment. In vitro experiments revealed that cargos on EVs could mediate macrophage polarization.</p>","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":""},"PeriodicalIF":2.9000,"publicationDate":"2025-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Extracellular Vesicles of Salivary Adenoid Cystic Carcinoma Suppress Immune Microenvironment in Lung.\",\"authors\":\"Yue Lou, Longping Liu, Yuping Liu, Zheng Zhou, Lijing Zhu, Yajuan Cui, Huaxiu Sun, Xuan Zhou, Chuanxiang Zhou, Tiejun Li\",\"doi\":\"10.1111/odi.70065\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>Salivary adenoid cystic carcinoma (SACC) frequently metastasizes to the lung and affects patient survival. Extracellular vesicles (EVs) have significant effects in the tumor pre-metastatic niche (PMN). In this study, we aimed to investigate immune changes in the lung pre-metastatic microenvironment of SACC.</p><p><strong>Methods: </strong>A pre-metastatic microenvironment was established using SACC-derived EVs at different time points. Labeled EVs were injected into mice to examine their different organ distributions. Flow cytometry was conducted to analyze the immune cell populations. In vitro, the same amount of SACC-derived EVs or EV-depleted conditional medium was added to macrophages to stimulate polarization.</p><p><strong>Results: </strong>The SACC-derived EVs colonized mostly in the lung and were engulfed by lung interstitial macrophages (IM). At different time points, the SACC-derived EVs induced decreased total macrophage, natural killer cell, and T cell populations, especially CD8<sup>+</sup> T cells. They also increased the M2 macrophage composition and regulatory T cell population. The in vitro study showed that SACC-derived EVs induce a mixed M1/M2 phenotype, but this effect was eliminated with the EV-depleted media.</p><p><strong>Conclusions: </strong>SACC-derived EVs circulate to the lung, where they are engulfed by IM and induce an immunosuppressive environment. In vitro experiments revealed that cargos on EVs could mediate macrophage polarization.</p>\",\"PeriodicalId\":19615,\"journal\":{\"name\":\"Oral diseases\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2025-08-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Oral diseases\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1111/odi.70065\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Oral diseases","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1111/odi.70065","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Extracellular Vesicles of Salivary Adenoid Cystic Carcinoma Suppress Immune Microenvironment in Lung.
Objective: Salivary adenoid cystic carcinoma (SACC) frequently metastasizes to the lung and affects patient survival. Extracellular vesicles (EVs) have significant effects in the tumor pre-metastatic niche (PMN). In this study, we aimed to investigate immune changes in the lung pre-metastatic microenvironment of SACC.
Methods: A pre-metastatic microenvironment was established using SACC-derived EVs at different time points. Labeled EVs were injected into mice to examine their different organ distributions. Flow cytometry was conducted to analyze the immune cell populations. In vitro, the same amount of SACC-derived EVs or EV-depleted conditional medium was added to macrophages to stimulate polarization.
Results: The SACC-derived EVs colonized mostly in the lung and were engulfed by lung interstitial macrophages (IM). At different time points, the SACC-derived EVs induced decreased total macrophage, natural killer cell, and T cell populations, especially CD8+ T cells. They also increased the M2 macrophage composition and regulatory T cell population. The in vitro study showed that SACC-derived EVs induce a mixed M1/M2 phenotype, but this effect was eliminated with the EV-depleted media.
Conclusions: SACC-derived EVs circulate to the lung, where they are engulfed by IM and induce an immunosuppressive environment. In vitro experiments revealed that cargos on EVs could mediate macrophage polarization.
期刊介绍：
Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.
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