从刺激周期中挽救卵细胞：改善卵母细胞形态和能力

Q3 Medicine

Morphologie Pub Date : 2025-08-18 DOI:10.1016/j.morpho.2025.101064

C. Hilali , N. Louanjli , M. Ennaji , K.H. Mohamed , N. Bouanani , E.M. Hissane , W.S. Rhazi , M. Zarquaoui , I. Kaarouch , R. Cabry , F.A. Lahlou , M. Benkhalifa

{"title":"从刺激周期中挽救卵细胞：改善卵母细胞形态和能力","authors":"C. Hilali , N. Louanjli , M. Ennaji , K.H. Mohamed , N. Bouanani , E.M. Hissane , W.S. Rhazi , M. Zarquaoui , I. Kaarouch , R. Cabry , F.A. Lahlou , M. Benkhalifa","doi":"10.1016/j.morpho.2025.101064","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><div>Oocyte in vitro maturation (<em>IVM</em>) is a technique where immature cumulus oocytes complexes (COCs) collected from mid cycle of follicular phase were maturated via Bi-Phasique maturation named CAPA-<em>IVM</em>or germinal vesicles (GV) and/or metaphase I oocytes (MI) from stimulated cycle are matured <em>in vitro</em>(<em>IVM</em> Rescue). The objective of our study was to evaluate the effectiveness of a new formulation of culture medium, developed by ourselves, in terms of improving oocyte maturation, competency, embryo development potential and quality.</div></div><div><h3>Material and methods</h3><div>This prospective study involved a total of 60 patients who underwent an <em>ICSI</em> cycle. Six hundred and fifty oocytes were collected including 410 oocytes at the metaphase II, 120 oocytes at the metaphase I and 120 oocytes at the germinal vesicle stages. Immature oocytes were divided into two groups: Group M (immature oocytes cultured in commercial<em>IVM</em> medium from Cooper-Origio) and group MS (immature oocytes cultured in the same medium but supplemented with an in-house mixture of cytokines, interleukins and growth factors). Metaphase II oocytes obtained after <em>IVM</em>underwent an <em>ICSI</em> attempt. Fertilization rates at day 1 and the numbers of 8-blastomere embryos at day 3 were assessed in all cultures after <em>ICSI</em>until blastocyst stage.</div></div><div><h3>Results</h3><div>While comparing <em>IVM</em> outcomes, we noted a significant increase in the oocyte maturation rate, fertilization rate and the percentage of 8-cell embryos on day 3 in the MS group compared to the M group. Moreover, the MS group also showed a significant increase in blastulation when compared to the MS group (<em>P</em>  =0.02).</div></div><div><h3>Conclusion</h3><div>The development of suitable <em>IVM</em> medium contributes to better rescue maturation of immature oocytes obtained in stimulated cycles. Its use may increase the number of good-quality embryos and thus can improve time to pregnancy and the cumulative pregnancy rate.</div></div>\",\"PeriodicalId\":39316,\"journal\":{\"name\":\"Morphologie\",\"volume\":\"109 367\",\"pages\":\"Article 101064\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-08-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Morphologie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S128601152500116X\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Morphologie","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S128601152500116X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 0

摘要

目的卵母细胞体外成熟（IVM）是一种从卵泡期中期收集的未成熟卵丘卵母细胞复合物（COCs）通过称为capa - ivmore生发囊泡（GV）和/或刺激周期中期的卵母细胞（MI）在体外成熟的技术（IVM Rescue）。本研究的目的是评价我们研制的一种新的培养基配方在提高卵母细胞成熟、能力、胚胎发育潜力和质量方面的有效性。材料和方法本前瞻性研究共涉及60例接受ICSI周期的患者。共收集卵母细胞650个，其中中期I期410个，中期I期120个，生发囊泡期120个。未成熟卵母细胞分为两组：M组（未成熟卵母细胞在cooper - origin的商用vm培养基中培养）和MS组（未成熟卵母细胞在相同的培养基中培养，但补充了细胞因子、白细胞介素和生长因子的混合物）。ivf行ICSI后获得的中期卵母细胞。在icsi后至囊胚期的所有培养中，评估第1天的受精率和第3天的8-卵裂球胚胎数量。结果在比较IVM结果时，我们注意到MS组第3天的卵母细胞成熟率、受精率和8细胞胚胎百分比明显高于M组。与MS组相比，MS组的囊胚数量也显著增加（P = 0.02）。结论培养合适的IVM培养液有助于刺激周期中未成熟卵母细胞的抢救成熟。它的使用可以增加优质胚胎的数量，从而可以延长妊娠时间和累积妊娠率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

IVMrescue from stimulated cycle: Improvement of oocyte morphology and competency

Objective

Oocyte in vitro maturation (IVM) is a technique where immature cumulus oocytes complexes (COCs) collected from mid cycle of follicular phase were maturated via Bi-Phasique maturation named CAPA-IVMor germinal vesicles (GV) and/or metaphase I oocytes (MI) from stimulated cycle are matured in vitro(IVM Rescue). The objective of our study was to evaluate the effectiveness of a new formulation of culture medium, developed by ourselves, in terms of improving oocyte maturation, competency, embryo development potential and quality.

Material and methods

This prospective study involved a total of 60 patients who underwent an ICSI cycle. Six hundred and fifty oocytes were collected including 410 oocytes at the metaphase II, 120 oocytes at the metaphase I and 120 oocytes at the germinal vesicle stages. Immature oocytes were divided into two groups: Group M (immature oocytes cultured in commercialIVM medium from Cooper-Origio) and group MS (immature oocytes cultured in the same medium but supplemented with an in-house mixture of cytokines, interleukins and growth factors). Metaphase II oocytes obtained after IVMunderwent an ICSI attempt. Fertilization rates at day 1 and the numbers of 8-blastomere embryos at day 3 were assessed in all cultures after ICSIuntil blastocyst stage.

Results

While comparing IVM outcomes, we noted a significant increase in the oocyte maturation rate, fertilization rate and the percentage of 8-cell embryos on day 3 in the MS group compared to the M group. Moreover, the MS group also showed a significant increase in blastulation when compared to the MS group (P = 0.02).

Conclusion

The development of suitable IVM medium contributes to better rescue maturation of immature oocytes obtained in stimulated cycles. Its use may increase the number of good-quality embryos and thus can improve time to pregnancy and the cumulative pregnancy rate.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Morphologie Medicine-Anatomy

CiteScore

2.30

自引率

0.00%

发文量

150

审稿时长

25 days

期刊介绍： Morphologie est une revue universitaire avec une ouverture médicale qui sa adresse aux enseignants, aux étudiants, aux chercheurs et aux cliniciens en anatomie et en morphologie. Vous y trouverez les développements les plus actuels de votre spécialité, en France comme a international. Le objectif de Morphologie est d?offrir des lectures privilégiées sous forme de revues générales, d?articles originaux, de mises au point didactiques et de revues de la littérature, qui permettront notamment aux enseignants de optimiser leurs cours et aux spécialistes d?enrichir leurs connaissances.