Muhammad Anamul Kabir, Sanzida Haque, Shishir Kumar Nandi, Tanwi Dey, Md Sakhawat Hossain, El-Sayed Hemdan Eissa, Martina Irwan Khoo, Zulhisyam Abdul Kari, Nor Dini Rusli, Hien Van Doan
{"title":"亚洲鲶鱼饲料中植物源粘合剂对生长、饲料质量、血液化学、肝脏和肠道健康以及经济效益的影响","authors":"Muhammad Anamul Kabir, Sanzida Haque, Shishir Kumar Nandi, Tanwi Dey, Md Sakhawat Hossain, El-Sayed Hemdan Eissa, Martina Irwan Khoo, Zulhisyam Abdul Kari, Nor Dini Rusli, Hien Van Doan","doi":"10.1038/s41598-025-16117-y","DOIUrl":null,"url":null,"abstract":"<p><p>Natural polysaccharides have demonstrated considerable potential as binding agents in aquafeed formulations, enhancing water stability and reducing nutrient leaching and pellet disintegration in aquatic environments. In this study, four isoproteic diets (32% crude protein) were formulated using different binders: carboxymethyl cellulose (D<sub>0</sub>, control), purple fingerling potato (D<sub>1</sub>), taro root (D<sub>2</sub>), and glutinous rice (D<sub>3</sub>). Each diet was administrated to Asian catfish (Clarias batrachus) (mean initial weight: 6.66 ± 0.01 g) over a 90-day trial to evaluate growth performance, feed stability, whole-body composition, health parameters, and economic feasibility. The results indicated that binder type significantly affected pellet durability index, floatability, and swelling rates (p < 0.05). Notably, fish fed the D<sub>2</sub> diet exhibited significantly higher (p < 0.05) feed attractability and palatability. Growth performance metrics, including final weight, weight gain, total biomass, specific growth rate, feed conversion ratio, and protein efficiency ratio, varied significantly (p < 0.05) among dietary treatments, with the D<sub>2</sub> group demonstrating the most favorable outcomes. Additionally, fish from the D<sub>2</sub> group exhibited significantly higher (p < 0.05) crude protein levels lower crude lipid content in whole-body composition. The dietary binders also had significant impact (p < 0.05) on hemato-biochemical indices, except for monocyte levels. Histological analysis indicated that fish in the D<sub>2</sub> and D<sub>3</sub> groups exhibited improved intestinal structure, characterised by increased villi length, a higher density of goblet cells, and an elongated lamina propria. In contrast, fish fed the D<sub>0</sub> and D<sub>3</sub> diets displayed increased vacuolar cytoplasm and degenerative nuclei. Furthermore, economic analysis revealed that the D<sub>2</sub> diet significantly enhanced economic efficiency (p < 0.05) compared to other treatments. In conclusion, incorporating taro root as a binder in the diet of Asian catfish (D<sub>2</sub>) improved growth performance, feed stability, health status, and economic returns, making it a promising alternative to conventional binders.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"29953"},"PeriodicalIF":3.9000,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12356898/pdf/","citationCount":"0","resultStr":"{\"title\":\"Effects of plant-origin binder sources in Asian catfish diets on growth, feed quality, blood chemistry, liver and gut health, and economic efficiency.\",\"authors\":\"Muhammad Anamul Kabir, Sanzida Haque, Shishir Kumar Nandi, Tanwi Dey, Md Sakhawat Hossain, El-Sayed Hemdan Eissa, Martina Irwan Khoo, Zulhisyam Abdul Kari, Nor Dini Rusli, Hien Van Doan\",\"doi\":\"10.1038/s41598-025-16117-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Natural polysaccharides have demonstrated considerable potential as binding agents in aquafeed formulations, enhancing water stability and reducing nutrient leaching and pellet disintegration in aquatic environments. In this study, four isoproteic diets (32% crude protein) were formulated using different binders: carboxymethyl cellulose (D<sub>0</sub>, control), purple fingerling potato (D<sub>1</sub>), taro root (D<sub>2</sub>), and glutinous rice (D<sub>3</sub>). Each diet was administrated to Asian catfish (Clarias batrachus) (mean initial weight: 6.66 ± 0.01 g) over a 90-day trial to evaluate growth performance, feed stability, whole-body composition, health parameters, and economic feasibility. The results indicated that binder type significantly affected pellet durability index, floatability, and swelling rates (p < 0.05). Notably, fish fed the D<sub>2</sub> diet exhibited significantly higher (p < 0.05) feed attractability and palatability. Growth performance metrics, including final weight, weight gain, total biomass, specific growth rate, feed conversion ratio, and protein efficiency ratio, varied significantly (p < 0.05) among dietary treatments, with the D<sub>2</sub> group demonstrating the most favorable outcomes. Additionally, fish from the D<sub>2</sub> group exhibited significantly higher (p < 0.05) crude protein levels lower crude lipid content in whole-body composition. The dietary binders also had significant impact (p < 0.05) on hemato-biochemical indices, except for monocyte levels. Histological analysis indicated that fish in the D<sub>2</sub> and D<sub>3</sub> groups exhibited improved intestinal structure, characterised by increased villi length, a higher density of goblet cells, and an elongated lamina propria. In contrast, fish fed the D<sub>0</sub> and D<sub>3</sub> diets displayed increased vacuolar cytoplasm and degenerative nuclei. Furthermore, economic analysis revealed that the D<sub>2</sub> diet significantly enhanced economic efficiency (p < 0.05) compared to other treatments. In conclusion, incorporating taro root as a binder in the diet of Asian catfish (D<sub>2</sub>) improved growth performance, feed stability, health status, and economic returns, making it a promising alternative to conventional binders.</p>\",\"PeriodicalId\":21811,\"journal\":{\"name\":\"Scientific Reports\",\"volume\":\"15 1\",\"pages\":\"29953\"},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2025-08-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12356898/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Scientific Reports\",\"FirstCategoryId\":\"103\",\"ListUrlMain\":\"https://doi.org/10.1038/s41598-025-16117-y\",\"RegionNum\":2,\"RegionCategory\":\"综合性期刊\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MULTIDISCIPLINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Scientific Reports","FirstCategoryId":"103","ListUrlMain":"https://doi.org/10.1038/s41598-025-16117-y","RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
Effects of plant-origin binder sources in Asian catfish diets on growth, feed quality, blood chemistry, liver and gut health, and economic efficiency.
Natural polysaccharides have demonstrated considerable potential as binding agents in aquafeed formulations, enhancing water stability and reducing nutrient leaching and pellet disintegration in aquatic environments. In this study, four isoproteic diets (32% crude protein) were formulated using different binders: carboxymethyl cellulose (D0, control), purple fingerling potato (D1), taro root (D2), and glutinous rice (D3). Each diet was administrated to Asian catfish (Clarias batrachus) (mean initial weight: 6.66 ± 0.01 g) over a 90-day trial to evaluate growth performance, feed stability, whole-body composition, health parameters, and economic feasibility. The results indicated that binder type significantly affected pellet durability index, floatability, and swelling rates (p < 0.05). Notably, fish fed the D2 diet exhibited significantly higher (p < 0.05) feed attractability and palatability. Growth performance metrics, including final weight, weight gain, total biomass, specific growth rate, feed conversion ratio, and protein efficiency ratio, varied significantly (p < 0.05) among dietary treatments, with the D2 group demonstrating the most favorable outcomes. Additionally, fish from the D2 group exhibited significantly higher (p < 0.05) crude protein levels lower crude lipid content in whole-body composition. The dietary binders also had significant impact (p < 0.05) on hemato-biochemical indices, except for monocyte levels. Histological analysis indicated that fish in the D2 and D3 groups exhibited improved intestinal structure, characterised by increased villi length, a higher density of goblet cells, and an elongated lamina propria. In contrast, fish fed the D0 and D3 diets displayed increased vacuolar cytoplasm and degenerative nuclei. Furthermore, economic analysis revealed that the D2 diet significantly enhanced economic efficiency (p < 0.05) compared to other treatments. In conclusion, incorporating taro root as a binder in the diet of Asian catfish (D2) improved growth performance, feed stability, health status, and economic returns, making it a promising alternative to conventional binders.
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