TP53和EZH2与唾液腺样囊性癌的关系。

IF 2 3区医学 Q2 Dentistry

Journal of Stomatology Oral and Maxillofacial Surgery Pub Date : 2025-08-12 DOI:10.1016/j.jormas.2025.102526

Yongbin Di, Haolei Zhang, Bohao Zhang, Dan Li

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引用次数: 0

摘要

背景：唾液腺腺样囊性癌是一种罕见的起源于唾液腺导管细胞的唾液腺癌，以局部浸润为特征。TP53、EZH2与唾液腺样囊性癌的关系尚不清楚。方法：从gene expression omnibus （GEO）下载唾液腺样囊性癌数据集GSE153002和GSE153283。筛选差异表达基因（DEGs）。进行加权基因共表达网络分析（WGCNA）、功能富集分析、基因集富集分析（GSEA）、蛋白质相互作用（PPI）网络构建与分析、比较毒物基因组学数据库（CTD）分析。绘制基因表达热图。采用RT-qPCR和WB检测验证核心基因的表达。结果：共获得98个交点deg。根据基因本体（Gene Ontology， GO），在生物过程（Biological Process， BP）分析中，它们主要富集于炎症反应、细胞周期、细胞迁移。在细胞组分分析中，它们主要富集于蛋白激酶复合体和含蛋白复合体。在分子功能（MF）分析中，它们主要集中在蛋白激酶活性和信号受体结合。在京都基因与基因组百科全书（KEGG）分析中，它们主要富集于肿瘤通路、MAPK、P53、PI3K-Akt信号通路、肿瘤转录失调。鉴定出28个重要模块，获得核心基因（TP53、EZH2）。基因表达热图显示TP53、EZH2在唾液腺样囊性癌中高表达。CTD分析显示TP53、EZH2与唾液腺肿瘤、腺癌、腺样囊性癌和炎症有关。RT-qPCR和WB检测结果显示，TP53和EZH2在唾液腺肿瘤组织中高表达。结论：TP53和EZH2在唾液腺样囊性癌中高表达，可能是其分子靶点。

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Association of TP53 and EZH2 with salivary adenoid cystic carcinoma.

Background: Adenoid cystic carcinoma of the salivary gland is a rare salivary gland carcinoma that originates from salivary duct cells and is characterized by local invasion. The relationship between TP53, EZH2 and salivary adenoid cystic carcinoma is still unclear.

Methods: Salivary adenoid cystic carcinoma datasets GSE153002 and GSE153283 were downloaded from gene expression omnibus (GEO). Differentially expressed genes (DEGs) were screened. Weighted gene co-expression network analysis (WGCNA), functional enrichment analysis, gene set enrichment analysis (GSEA), construction and analysis of protein protein interaction (PPI) network, Comparative Toxicogenomics Database (CTD) analysis were performed. Gene expression heatmap was drawn. Using RT-qPCR and WB assays to validate the expression of core genes.

Results: 98 intersection DEGs were obtained. According to Gene Ontology (GO), in Biological Process (BP) analysis, they are primarily enriched in inflammatory response, cell cycle, cell migration. In Cellular Component (CC) analysis, they mainly enrich in protein kinase complex and protein-containing complex. In Molecular Function (MF) analysis, they are concentrated in protein kinase activity and signal receptor binding. In Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, they mainly enrich in cancer pathways, MAPK, P53, PI3K-Akt signaling pathway, transcriptional dysregulation in cancer. Twenty-eight significant modules were identified, core genes (TP53, EZH2) were obtained. The gene expression heatmap reveals high expression of TP53, EZH2 in salivary adenoid cystic carcinoma samples. CTD analysis indicates the association of TP53, EZH2 with salivary gland neoplasms, adenocarcinoma, adenoid cystic carcinoma, and inflammation. Results from RT-qPCR and WB assays showed that TP53 and EZH2 were highly expressed in salivary gland tumor tissues.

Conclusion: TP53 and EZH2 are highly expressed in salivary adenoid cystic carcinoma, which may serve as their molecular targets.

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来源期刊

Journal of Stomatology Oral and Maxillofacial Surgery Dentistry-Oral Surgery

CiteScore

2.20

自引率

9.10%

发文量

305

期刊介绍： J Stomatol Oral Maxillofac Surg publishes research papers and techniques - (guest) editorials, original articles, reviews, technical notes, case reports, images, letters to the editor, guidelines - dedicated to enhancing surgical expertise in all fields relevant to oral and maxillofacial surgery: from plastic and reconstructive surgery of the face, oral surgery and medicine, … to dentofacial and maxillofacial orthopedics. Original articles include clinical or laboratory investigations and clinical or equipment reports. Reviews include narrative reviews, systematic reviews and meta-analyses. All manuscripts submitted to the journal are subjected to peer review by international experts, and must: Be written in excellent English, clear and easy to understand, precise and concise; Bring new, interesting, valid information - and improve clinical care or guide future research; Be solely the work of the author(s) stated; Not have been previously published elsewhere and not be under consideration by another journal; Be in accordance with the journal''s Guide for Authors'' instructions: manuscripts that fail to comply with these rules may be returned to the authors without being reviewed. Under no circumstances does the journal guarantee publication before the editorial board makes its final decision. The journal is indexed in the main international databases and is accessible worldwide through the ScienceDirect and ClinicalKey Platforms.