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引用次数: 0
摘要
背景：本研究旨在寻找脓毒症早期诊断和治疗的panoptosis相关基因标记物。方法：从Gene Expression Omnibus （GEO）数据库中收集转录数据集，使用R语言和“limma”软件包进行差异表达分析。使用“clusterProfiler”软件包进行功能富集分析，并进行蛋白质-蛋白质相互作用（PPI）分析。使用FIMO工具预测转录因子（TF）结合位点。进行基因集富集分析（GSEA）和疾病本体分析（DO）。免疫浸润分析采用CIBERSORT、ssGSEA和xCell算法。结果：共发现18个panoptois相关基因在脓毒症与正常样本中表达有显著差异，选择APAF1作为靶基因。APAF1在脓毒症中的表达高于正常样本。ROC分析显示其诊断价值。TF HIF1A和4种mirna可能是APAF1的调节因子。APAF1与CD8 T细胞和静息NK细胞呈负相关，与中性粒细胞、巨噬细胞M0、T细胞γ δ和浆细胞呈正相关。许多靶标药物对APAF1及其相关tf均有高敏感性。结论：panoposis相关基因APAF1在脓毒症中高表达，具有一定的诊断价值。
PANoptosis-related gene APAF1 may contribute to the progression of sepsis.
Background: This research tried to identify a PANoptosis-related gene marker for sepsis early diagnosis and treatment.
Methods: We collected transcriptional datasets from the Gene Expression Omnibus (GEO) database and performed differential expression analysis using the R language and the "limma" package. Functional enrichment analysis was conducted using the "clusterProfiler" package, and Protein-Protein Interaction (PPI) analysis was carried out. Transcription factor (TF) binding sites were predicted using FIMO tool. Gene set enrichment analysis (GSEA) and disease ontology (DO) analysis were performed. Immune infiltration analysis was conducted using CIBERSORT, ssGSEA, and the xCell algorithm.
Results: A total of 18 PANoptosis-related genes were found to express significantly differentially between sepsis and normal samples, and APAF1 was selected as the target gene. APAF1 expressed higher in sepsis compared to normal samples. ROC analysis indicated its diagnostic value. TF HIF1A and 4 miRNAs might be regulators of APAF1. APAF1 was negatively related to CD8 T cells and resting NK cells, and positively related neutrophils, macrophages M0, T cells gamma delta, and plasma cells. Many target drugs were detected high sensitivity to APAF1 and its related TFs.
Conclusion: PANoptosis-related gene APAF1 was identified to highly express in sepsis and it was valuable in diagnosis.
期刊介绍：
The Irish Journal of Medical Science is the official organ of the Royal Academy of Medicine in Ireland. Established in 1832, this quarterly journal is a contribution to medical science and an ideal forum for the younger medical/scientific professional to enter world literature and an ideal launching platform now, as in the past, for many a young research worker.
The primary role of both the Academy and IJMS is that of providing a forum for the exchange of scientific information and to promote academic discussion, so essential to scientific progress.
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