{"title":"HPV基因型感染模式对高级别鳞状上皮内病变发生影响的研究。","authors":"Qiao Zhou, Kekai Luo, Liangping Zhao","doi":"10.1002/dc.70005","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Objective</h3>\n \n <p>To investigate the differential risk of high-grade squamous intraepithelial lesion or worse (HSIL+) associated with single versus co-infection patterns of high-risk human papillomavirus (HR-HPV) genotypes.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>In this retrospective cohort study, 10,570 patients with abnormal ThinPrep cytology test results and/or HR-HPV infection who underwent colposcopy-guided cervical biopsy at Wuhan Children's Hospital (May 2021–May 2023) were enrolled. Histopathological diagnosis served as the gold standard. Multivariate logistic regression was used to analyze HSIL+ risk across HPV infection patterns, adjusting for age and viral load.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Single infections with HPV31, HPV33, or HPV58 demonstrate comparable positivity rates of HSIL+ to HPV16 monoinfection. After adjusting for confounders, logistic regression revealed that co-infection of HPV16 with low-risk HPV genotypes reduced the risk of progression to HSIL+ compared to HPV16 monoinfection (<i>p</i> < 0.05). Similarly, co-infections involving HPV33 or HPV58 (regardless of high/low-risk partners) were associated with lower HSIL+ risk (all <i>p</i> < 0.05). In contrast, HPV31 demonstrated consistent HSIL+ risk irrespective of co-infection status.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>HPV16, HPV31, HPV33, or HPV58 need equivalent clinical vigilance in screening and management protocols. Co-infection with low-risk HPV genotypes attenuates HSIL+ risk in HPV16-infected individuals, and HPV33/58 co-infections (with any genotype) exhibit protective effects. Our study suggests that HPV31-associated risk might remain unaffected by co-infection, suggesting genotype-specific biological interactions. These findings highlight the importance of genotyping-guided risk stratification in cervical cancer screening.</p>\n </section>\n </div>","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":"53 11","pages":"529-535"},"PeriodicalIF":1.0000,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A Study on the Impact of HPV Genotype Infection Patterns on the Occurrence of High-Grade Squamous Intraepithelial Lesion\",\"authors\":\"Qiao Zhou, Kekai Luo, Liangping Zhao\",\"doi\":\"10.1002/dc.70005\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Objective</h3>\\n \\n <p>To investigate the differential risk of high-grade squamous intraepithelial lesion or worse (HSIL+) associated with single versus co-infection patterns of high-risk human papillomavirus (HR-HPV) genotypes.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>In this retrospective cohort study, 10,570 patients with abnormal ThinPrep cytology test results and/or HR-HPV infection who underwent colposcopy-guided cervical biopsy at Wuhan Children's Hospital (May 2021–May 2023) were enrolled. Histopathological diagnosis served as the gold standard. Multivariate logistic regression was used to analyze HSIL+ risk across HPV infection patterns, adjusting for age and viral load.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>Single infections with HPV31, HPV33, or HPV58 demonstrate comparable positivity rates of HSIL+ to HPV16 monoinfection. After adjusting for confounders, logistic regression revealed that co-infection of HPV16 with low-risk HPV genotypes reduced the risk of progression to HSIL+ compared to HPV16 monoinfection (<i>p</i> < 0.05). Similarly, co-infections involving HPV33 or HPV58 (regardless of high/low-risk partners) were associated with lower HSIL+ risk (all <i>p</i> < 0.05). In contrast, HPV31 demonstrated consistent HSIL+ risk irrespective of co-infection status.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion</h3>\\n \\n <p>HPV16, HPV31, HPV33, or HPV58 need equivalent clinical vigilance in screening and management protocols. Co-infection with low-risk HPV genotypes attenuates HSIL+ risk in HPV16-infected individuals, and HPV33/58 co-infections (with any genotype) exhibit protective effects. Our study suggests that HPV31-associated risk might remain unaffected by co-infection, suggesting genotype-specific biological interactions. These findings highlight the importance of genotyping-guided risk stratification in cervical cancer screening.</p>\\n </section>\\n </div>\",\"PeriodicalId\":11349,\"journal\":{\"name\":\"Diagnostic Cytopathology\",\"volume\":\"53 11\",\"pages\":\"529-535\"},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2025-08-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Diagnostic Cytopathology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/dc.70005\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MEDICAL LABORATORY TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Diagnostic Cytopathology","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/dc.70005","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MEDICAL LABORATORY TECHNOLOGY","Score":null,"Total":0}
A Study on the Impact of HPV Genotype Infection Patterns on the Occurrence of High-Grade Squamous Intraepithelial Lesion
Objective
To investigate the differential risk of high-grade squamous intraepithelial lesion or worse (HSIL+) associated with single versus co-infection patterns of high-risk human papillomavirus (HR-HPV) genotypes.
Methods
In this retrospective cohort study, 10,570 patients with abnormal ThinPrep cytology test results and/or HR-HPV infection who underwent colposcopy-guided cervical biopsy at Wuhan Children's Hospital (May 2021–May 2023) were enrolled. Histopathological diagnosis served as the gold standard. Multivariate logistic regression was used to analyze HSIL+ risk across HPV infection patterns, adjusting for age and viral load.
Results
Single infections with HPV31, HPV33, or HPV58 demonstrate comparable positivity rates of HSIL+ to HPV16 monoinfection. After adjusting for confounders, logistic regression revealed that co-infection of HPV16 with low-risk HPV genotypes reduced the risk of progression to HSIL+ compared to HPV16 monoinfection (p < 0.05). Similarly, co-infections involving HPV33 or HPV58 (regardless of high/low-risk partners) were associated with lower HSIL+ risk (all p < 0.05). In contrast, HPV31 demonstrated consistent HSIL+ risk irrespective of co-infection status.
Conclusion
HPV16, HPV31, HPV33, or HPV58 need equivalent clinical vigilance in screening and management protocols. Co-infection with low-risk HPV genotypes attenuates HSIL+ risk in HPV16-infected individuals, and HPV33/58 co-infections (with any genotype) exhibit protective effects. Our study suggests that HPV31-associated risk might remain unaffected by co-infection, suggesting genotype-specific biological interactions. These findings highlight the importance of genotyping-guided risk stratification in cervical cancer screening.
期刊介绍:
Diagnostic Cytopathology is intended to provide a forum for the exchange of information in the field of cytopathology, with special emphasis on the practical, clinical aspects of the discipline. The editors invite original scientific articles, as well as special review articles, feature articles, and letters to the editor, from laboratory professionals engaged in the practice of cytopathology. Manuscripts are accepted for publication on the basis of scientific merit, practical significance, and suitability for publication in a journal dedicated to this discipline. Original articles can be considered only with the understanding that they have never been published before and that they have not been submitted for simultaneous review to another publication.