Mosammat Rezaun Nahar , Syeda Anjuman Nasreen , Shyamal Kumar Paul , Mohammed Abedul Hoque , Meiji Soe Aung , Nazia Haque , Tasmia Hossain , Sheikh Anika Tasnim , Sultana Jahan Tuly , Nashid Sultana Ishi , Iffat Ara Ifa , Abdullah Al Mamun , Parvez Arafa , Nobumichi Kobayashi
{"title":"孟加拉国引起持续性手术部位感染的非结核分枝杆菌的流行病学特征","authors":"Mosammat Rezaun Nahar , Syeda Anjuman Nasreen , Shyamal Kumar Paul , Mohammed Abedul Hoque , Meiji Soe Aung , Nazia Haque , Tasmia Hossain , Sheikh Anika Tasnim , Sultana Jahan Tuly , Nashid Sultana Ishi , Iffat Ara Ifa , Abdullah Al Mamun , Parvez Arafa , Nobumichi Kobayashi","doi":"10.1016/j.ijregi.2025.100714","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><div>Nontuberculous mycobacteria (NTM) are an increasing cause of extrapulmonary infections affecting skin and soft tissue. This study aimed to determine the involvement of NTM in persistent surgical site infections (SSIs) in Bangladesh.</div></div><div><h3>Methods</h3><div>Specimens of SSIs (wound swab, pus, sinus discharge) were collected from patients who attended a tertiary care hospital during a 6-month period in 2024. NTM were detected by phenotypic methods (microscopic examination, culture, biochemical tests), immunochromatography, and polymerase chain reaction. Species of NTM were identified by sequence analysis of <em>hps65</em> gene. Antimicrobial susceptibility was determined by broth microdilution test.</div></div><div><h3>Results</h3><div>Among a total of 155 samples collected, NTM were detected in 12 samples (7.7%), which were identified to be <em>Mycobacterium abscessus</em> (n = 5), <em>Mycobacterium fortuitum</em> (n = 4), <em>Mycobacterium intracellulare, Mycobacterium engbaekii</em>, and <em>Mycobacterium kubicae</em> (one specimen each). NTM were detected in patients aged 0-50 years who were showing variable durations of non-healing wound (3 to >14 weeks), more commonly after surgery of laparoscopic cholecystectomy and laparotomy. Resistance to amikacin, ciprofloxacin, and clarithromycin was noted for <em>M. abscessus</em> and <em>M. fortuitum</em>.</div></div><div><h3>Conclusions</h3><div>NTM represented by <em>M. abscessus</em> and <em>M. fortuitum</em> were revealed to be a significant cause of persistent SSIs of various patient groups in Bangladesh, associated with specific types of surgery.</div></div>","PeriodicalId":73335,"journal":{"name":"IJID regions","volume":"16 ","pages":"Article 100714"},"PeriodicalIF":1.7000,"publicationDate":"2025-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Prevalence and epidemiologic features of nontuberculous mycobacteria causing persistent surgical site infections in Bangladesh\",\"authors\":\"Mosammat Rezaun Nahar , Syeda Anjuman Nasreen , Shyamal Kumar Paul , Mohammed Abedul Hoque , Meiji Soe Aung , Nazia Haque , Tasmia Hossain , Sheikh Anika Tasnim , Sultana Jahan Tuly , Nashid Sultana Ishi , Iffat Ara Ifa , Abdullah Al Mamun , Parvez Arafa , Nobumichi Kobayashi\",\"doi\":\"10.1016/j.ijregi.2025.100714\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objectives</h3><div>Nontuberculous mycobacteria (NTM) are an increasing cause of extrapulmonary infections affecting skin and soft tissue. This study aimed to determine the involvement of NTM in persistent surgical site infections (SSIs) in Bangladesh.</div></div><div><h3>Methods</h3><div>Specimens of SSIs (wound swab, pus, sinus discharge) were collected from patients who attended a tertiary care hospital during a 6-month period in 2024. NTM were detected by phenotypic methods (microscopic examination, culture, biochemical tests), immunochromatography, and polymerase chain reaction. Species of NTM were identified by sequence analysis of <em>hps65</em> gene. Antimicrobial susceptibility was determined by broth microdilution test.</div></div><div><h3>Results</h3><div>Among a total of 155 samples collected, NTM were detected in 12 samples (7.7%), which were identified to be <em>Mycobacterium abscessus</em> (n = 5), <em>Mycobacterium fortuitum</em> (n = 4), <em>Mycobacterium intracellulare, Mycobacterium engbaekii</em>, and <em>Mycobacterium kubicae</em> (one specimen each). NTM were detected in patients aged 0-50 years who were showing variable durations of non-healing wound (3 to >14 weeks), more commonly after surgery of laparoscopic cholecystectomy and laparotomy. Resistance to amikacin, ciprofloxacin, and clarithromycin was noted for <em>M. abscessus</em> and <em>M. fortuitum</em>.</div></div><div><h3>Conclusions</h3><div>NTM represented by <em>M. abscessus</em> and <em>M. fortuitum</em> were revealed to be a significant cause of persistent SSIs of various patient groups in Bangladesh, associated with specific types of surgery.</div></div>\",\"PeriodicalId\":73335,\"journal\":{\"name\":\"IJID regions\",\"volume\":\"16 \",\"pages\":\"Article 100714\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2025-07-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"IJID regions\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2772707625001493\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"IJID regions","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2772707625001493","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Prevalence and epidemiologic features of nontuberculous mycobacteria causing persistent surgical site infections in Bangladesh
Objectives
Nontuberculous mycobacteria (NTM) are an increasing cause of extrapulmonary infections affecting skin and soft tissue. This study aimed to determine the involvement of NTM in persistent surgical site infections (SSIs) in Bangladesh.
Methods
Specimens of SSIs (wound swab, pus, sinus discharge) were collected from patients who attended a tertiary care hospital during a 6-month period in 2024. NTM were detected by phenotypic methods (microscopic examination, culture, biochemical tests), immunochromatography, and polymerase chain reaction. Species of NTM were identified by sequence analysis of hps65 gene. Antimicrobial susceptibility was determined by broth microdilution test.
Results
Among a total of 155 samples collected, NTM were detected in 12 samples (7.7%), which were identified to be Mycobacterium abscessus (n = 5), Mycobacterium fortuitum (n = 4), Mycobacterium intracellulare, Mycobacterium engbaekii, and Mycobacterium kubicae (one specimen each). NTM were detected in patients aged 0-50 years who were showing variable durations of non-healing wound (3 to >14 weeks), more commonly after surgery of laparoscopic cholecystectomy and laparotomy. Resistance to amikacin, ciprofloxacin, and clarithromycin was noted for M. abscessus and M. fortuitum.
Conclusions
NTM represented by M. abscessus and M. fortuitum were revealed to be a significant cause of persistent SSIs of various patient groups in Bangladesh, associated with specific types of surgery.
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