α -蒎烯在溃疡性结肠炎模型大鼠NLRP3-Caspase 1通路中的作用

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2025-08-14 DOI:10.1016/j.tice.2025.103085

Kaveh Rahimi , Annahita Rezaie , Arian Javadi , Mahsa Khademi Kalantari , Negar Eftekhar , Hoora Shushtari

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引用次数: 0

摘要

α -蒎烯是一种天然化合物，由于其抗炎和抗氧化作用，最近引起了研究人员的注意。本研究旨在探讨α -蒎烯对结肠炎模型结肠组织NLRP3-Caspase 1通路活性的影响。采用4 %醋酸（A.A）直接注入结肠诱导结肠炎。处理24小时后，口服试验化合物α -蒎烯（剂量分别为50和100 mg/kg）、磺胺吡啶（剂量为50 mg/kg）或载药（液体石蜡）。结肠炎诱导7天后，检测结肠各节段疾病评分指数、结肠重长比变化、氧化应激因子、NLRP3-Caspase 1通路活性。α -蒎烯改善疾病评分指数和结肠重长比。α -蒎烯通过降低丙二醛水平和增加GSH、SOD和CAT水平来平衡醋酸性结肠炎中的氧化应激因子。此外，α -蒎烯通过降低MAPK P38活性降低NLRP3通路活性，导致NF-KB、NLRP3和Caspase 1表达降低，IL-1B和IL-18水平降低。我们的研究表明，α -蒎烯可能通过降低氧化应激和降低NLRP3 caspase-1通路的活性来有效治疗结肠炎。

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The role of alpha-pinene on the NLRP3-Caspase 1 pathway in the ulcerative colitis model in rats

Alpha-pinene is a natural compound that has recently attracted the attention of researchers due to its anti-inflammatory and antioxidant effects. This study aimed to investigate the impact of alpha-pinene on the NLRP3-Caspase 1 pathway activity in colon tissue in a colitis model. The colitis was induced by directly administering 4 % acetic acid (A.A) into the colon. Twenty-four hours after this treatment, the test compound alpha-pinene (at doses of 50 and 100 mg/kg), sulfasalazine (50 mg/kg), or the vehicle (liquid paraffin) was given orally. Seven days after inducing colitis, the colonic segments were examined for disease score index, changes in the colon weight/length ratio, oxidative stress factors, and NLRP3-Caspase 1 pathway activity. Alpha-pinene improved the disease score index and the colon weight/length ratio. Alpha-pinene balanced oxidative stress factors in acetic acid-induced colitis by reducing MDA levels and increasing GSH, SOD, and CAT levels. Additionally, alpha-pinene decreased NLRP3 pathway activity by reducing MAPK P38 activity, leading to decreased expression of NF-KB, NLRP3, and Caspase 1, as well as decreased levels of IL-1B and IL-18. Our research suggests that alpha-pinene may be effective in treating colitis by reducing oxidative stress and decreasing the activity of the NLRP3 caspase-1 pathway.

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.