犬iPSC技术的进展：目前的方法和未来的发展方向

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science Pub Date : 2025-08-11 DOI:10.1016/j.rvsc.2025.105844

Pruettha Aruvornlop , Warunya Chakritbudsabong , Nichawadee Sandech , Tharathip Muangthong , Sasitorn Rungarunlert

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引用次数: 0

摘要

犬诱导多能干细胞（ciPSCs）在兽医再生医学和人类疾病的转化模型方面具有重要的前景。从体细胞来源获得这些细胞也规避了与胚胎材料相关的伦理问题。然而，该领域的进展受到诸如重编程效率低、缺乏标准化、独特的表观遗传障碍和不明确的多能性状态等挑战的阻碍。这篇叙述性综述提供了一个全面的和最新的关于ciPSC生成和表征的当前方法的概述，强调了供体细胞选择、重编程方法、培养条件和分析技术的最新进展。此外，综述探讨了再生医学和疾病建模的新兴应用，讨论了未来的潜力和当前的局限性。通过改进方案和有效的差异化策略来解决这些挑战，对于充分释放ciPSC技术的研究和治疗价值至关重要。

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Advances in canine iPSC technology: Current methods and future directions — A narrative review

Canine induced pluripotent stem cells (ciPSCs) hold significant promise for veterinary regenerative medicine and as translational models for human diseases. Deriving these cells from somatic sources also circumvents ethical concerns associated with embryonic material. However, progress in the field is impeded by challenges such as low reprogramming efficiency, lack of standardization, unique epigenetic barriers, and ambiguous pluripotency states. This narrative review provides a comprehensive and up-to-date overview of current approaches to ciPSC generation and characterization, emphasizing recent advancements in donor cell selection, reprogramming methodologies, culture conditions, and analytical techniques. Furthermore, the review explores emerging applications in regenerative medicine and disease modeling, discussing both future potential and current limitations. Addressing these challenges through improved protocols and effective differentiation strategies is crucial to fully unlocking ciPSC technology's research and therapeutic value.

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来源期刊

Research in veterinary science 农林科学-兽医学

CiteScore

4.40

自引率

4.20%

发文量

312

审稿时长

75 days

期刊介绍： Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research. The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally. High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health. Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.