BKPyV在阿根廷引起HIV/AIDS患者脑膜脑炎

IF 1.8 4区医学 Q3 INFECTIOUS DISEASES

Diagnostic microbiology and infectious disease Pub Date : 2025-08-12 DOI:10.1016/j.diagmicrobio.2025.117063

Viviana Raskovsky , Sebastián Blanco , Federico Perrote , Esteban Lavaque , Adrián Edelcopp , María C. Frutos , Sandra Gallego

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引用次数: 0

摘要

艾滋病毒/艾滋病患者中枢神经系统（CNS）的JCPyV/BKPyV机会性感染增加了发病率/死亡率，特别是在低收入国家。在阿根廷，关于艾滋病毒/艾滋病中多瘤病毒感染中枢神经系统的数据很少。我们回顾性评估了阿根廷萨尔塔省艾滋病死亡率高的艾滋病毒/艾滋病脑膜脑炎或进行性多灶性脑白质病（PML）患者脑脊液（CSF）样本中的BKPyV/JCPyV感染情况。对所有在某地区转诊医院住院一年的BKPyV/JCPyV HIV/AIDS患者冷冻保存的脑脊液样本，采用PCR扩增173 pb t抗原序列，寻找多瘤病毒DNA。随后，通过直接测序进行多瘤病毒基因分型。6%（3/50）的中枢神经系统疾病患者携带多瘤病毒；脑膜脑炎1例合并BKPyV， PML 2例合并JCPyV。鉴定出1个基因型为2A。据我们所知，这是阿根廷首次描述艾滋病毒/艾滋病患者中bkpyv相关的脑膜脑炎，以及该国首次报告导致艾滋病毒/艾滋病患者神经系统疾病的JCPyV基因2型。这些数据支持将BKPyV /JCPyV分子诊断系统地纳入阿根廷艾滋病毒/艾滋病和神经系统疾病患者脑脊液常规筛查。在这些患者中，中枢神经系统疾病的病因学诊断是最重要的。

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BKPyV causing meningoencephalitis in HIV/AIDS patients in Argentina

Opportunistic infections in the central nervous system (CNS) by JCPyV/BKPyV in HIV/AIDS patients increase morbidity/mortality, especially in low-income countries. In Argentina, data regarding CNS-infection by polyomavirus in HIV/AIDS are scarce. We retrospectively evaluated BKPyV/JCPyV infections in cerebrospinal fluid (CSF) samples of HIV/AIDS patients with meningoencephalitis or Progressive Multifocal Leukoencephalopathy (PML) from Salta, Argentina, a province with high rates of mortality from AIDS.

Cryopreserved CSF samples from all HIV/AIDS patients with BKPyV/JCPyV admitted to a regional referral hospital for one year underwent amplification of 173 pb T-antigen sequence by PCR, searching for polyomavirus DNA. Subsequently, polyomavirus genotyping by direct sequencing was performed.

Six percent (3/50) of the patients with CNS disorders had polyomavirus; one patient with meningoencephalitis had BKPyV, and 2 patients with PML had JCPyV. One genotype 2A was identified.

To our knowledge, this is the first description of BKPyV-associated meningoencephalitis in HIV/AIDS patients in Argentina, together with the first report of JCPyV genotype 2 responsible for neurological disease in a patient with HIV/AIDS in the country. These data support the systematic incorporation of molecular diagnosis for BKPyV /JCPyV into routine screening of CSF in patients with HIV/AIDS and neurological diseases in Argentina. Etiological diagnosis of CNS diseases in these patients is overriding.

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来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.