Mahmoud G. Soliman, Jennifer Fernandez Alarcon, Tanja Ursula Lüdtke, Martina B. Violatto, Marko Dobricic, Chiara Cordiglieri, Alessandro Corbelli, Fabio Fiordaliso, Giovanni Sitia, James S. O'Donnell, Daniel I. R. Spencer, Sergio Moya, Paolo Bigini and Marco P. Monopoli
{"title":"用糖肽修饰金纳米颗粒可降低细胞摄取和肝脏滞留。","authors":"Mahmoud G. Soliman, Jennifer Fernandez Alarcon, Tanja Ursula Lüdtke, Martina B. Violatto, Marko Dobricic, Chiara Cordiglieri, Alessandro Corbelli, Fabio Fiordaliso, Giovanni Sitia, James S. O'Donnell, Daniel I. R. Spencer, Sergio Moya, Paolo Bigini and Marco P. Monopoli","doi":"10.1039/D5NA00464K","DOIUrl":null,"url":null,"abstract":"<p >Unspecific uptake by the liver is one of the main drawbacks of the translation of nanomaterials into clinics, preventing their delivery into diseased tissues. Here, we synthesized gold nanoparticles (GNPs) decorated with a sialic acid-displaying glycopeptide to enhance their specific targeting properties by reducing their uptake inside hepatic cells. We demonstrated the biocompatibility of the glycopeptide-coated GNPs with two different nanomaterial shapes (spherical and rod-like GNPs) and the targeting properties of the glycopeptide were retained in serum-free and protein-rich media. We found that the glycopeptide reduces nanomaterial interaction with hepatic cells by 1.96 times. In the liver, Kupffer cells (KCs) and liver sinusoidal endothelial cells (LSECs) were the only cells that interacted with the GNPs, increasing the expression of sialic acid-binding receptors such as Siglec-1. This work provides potential new strategies to overcome off-target nanomaterial accumulation by manipulating nanomaterial functionalisation with glycans to alter hepatic cell interactions.</p>","PeriodicalId":18806,"journal":{"name":"Nanoscale Advances","volume":" 18","pages":" 5784-5798"},"PeriodicalIF":4.6000,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12341466/pdf/","citationCount":"0","resultStr":"{\"title\":\"Decoration of gold nanoparticles with glycopeptides leads to a lower cellular uptake and liver retention†\",\"authors\":\"Mahmoud G. Soliman, Jennifer Fernandez Alarcon, Tanja Ursula Lüdtke, Martina B. Violatto, Marko Dobricic, Chiara Cordiglieri, Alessandro Corbelli, Fabio Fiordaliso, Giovanni Sitia, James S. O'Donnell, Daniel I. R. Spencer, Sergio Moya, Paolo Bigini and Marco P. Monopoli\",\"doi\":\"10.1039/D5NA00464K\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Unspecific uptake by the liver is one of the main drawbacks of the translation of nanomaterials into clinics, preventing their delivery into diseased tissues. Here, we synthesized gold nanoparticles (GNPs) decorated with a sialic acid-displaying glycopeptide to enhance their specific targeting properties by reducing their uptake inside hepatic cells. We demonstrated the biocompatibility of the glycopeptide-coated GNPs with two different nanomaterial shapes (spherical and rod-like GNPs) and the targeting properties of the glycopeptide were retained in serum-free and protein-rich media. We found that the glycopeptide reduces nanomaterial interaction with hepatic cells by 1.96 times. In the liver, Kupffer cells (KCs) and liver sinusoidal endothelial cells (LSECs) were the only cells that interacted with the GNPs, increasing the expression of sialic acid-binding receptors such as Siglec-1. This work provides potential new strategies to overcome off-target nanomaterial accumulation by manipulating nanomaterial functionalisation with glycans to alter hepatic cell interactions.</p>\",\"PeriodicalId\":18806,\"journal\":{\"name\":\"Nanoscale Advances\",\"volume\":\" 18\",\"pages\":\" 5784-5798\"},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2025-08-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12341466/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nanoscale Advances\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://pubs.rsc.org/en/content/articlelanding/2025/na/d5na00464k\",\"RegionNum\":3,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nanoscale Advances","FirstCategoryId":"88","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2025/na/d5na00464k","RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Decoration of gold nanoparticles with glycopeptides leads to a lower cellular uptake and liver retention†
Unspecific uptake by the liver is one of the main drawbacks of the translation of nanomaterials into clinics, preventing their delivery into diseased tissues. Here, we synthesized gold nanoparticles (GNPs) decorated with a sialic acid-displaying glycopeptide to enhance their specific targeting properties by reducing their uptake inside hepatic cells. We demonstrated the biocompatibility of the glycopeptide-coated GNPs with two different nanomaterial shapes (spherical and rod-like GNPs) and the targeting properties of the glycopeptide were retained in serum-free and protein-rich media. We found that the glycopeptide reduces nanomaterial interaction with hepatic cells by 1.96 times. In the liver, Kupffer cells (KCs) and liver sinusoidal endothelial cells (LSECs) were the only cells that interacted with the GNPs, increasing the expression of sialic acid-binding receptors such as Siglec-1. This work provides potential new strategies to overcome off-target nanomaterial accumulation by manipulating nanomaterial functionalisation with glycans to alter hepatic cell interactions.