复发性妊娠糖尿病的肠道微生物组和血清代谢改变。

IF 7.4 3区医学 Q1 ENDOCRINOLOGY & METABOLISM

Diabetes research and clinical practice Pub Date : 2025-08-11 DOI:10.1016/j.diabres.2025.112416

Wei Zheng , Yixuan Lu , Xianxian Yuan , Ruihua Yang , Yujie Zhang , Li Zhang , Guanghui Li

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引用次数: 0

摘要

目的：本研究旨在确定复发性妊娠糖尿病（GDM）妇女的肠道微生物组和血清代谢改变，这对有既往病史的妇女有很大影响。方法：来自中国北京的70名既往患有GDM的孕妇参与了这项前瞻性队列研究。在妊娠早期（8-12 周）和妊娠中期（24-28 周）采集粪便和血清样本。通过16S rRNA测序分析肠道微生物群组成，使用非靶向代谢组学分析血清代谢物。孟德尔随机化（MR）分析了来自芬兰、加拿大和英国生物银行队列的综合数据，以推断潜在的因果关系。结果：复发性GDM病例表现出特定阶段的微生物生态失调，其特征是早期促炎类群（如链球菌、肠杆菌）的富集和有益拟杆菌的减少，随后是妊娠中期乳酸菌科和脱硫弧菌的减少。网络分析发现复发性GDM中微生物群落凝聚力降低。代谢组学分析显示复发性GDM中咖啡因代谢物和脂质的下调。MR分析表明，16个分类群可能通过脂质信号等代谢物介导影响GDM风险。结论：这些发现强调了通过调节妊娠期间肠道微生物群和代谢副产物来预防GDM的潜在干预措施。

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Gut microbiome and serum metabolic alterations in recurrent gestational diabetes mellitus

Aim

This study aimed to identify gut microbiome and serum metabolic alterations in women with recurrent gestational diabetes mellitus (GDM), which affects a significant proportion of those with a prior history.

Methods

Seventy pregnant women with prior GDM from Beijing, China, participated in this prospective cohort study. Fecal and serum samples were collected in the first (8–12 weeks) and second trimesters (24–28 weeks). Gut microbiota composition was analyzed via 16S rRNA sequencing, and serum metabolites were profiled using untargeted metabolomics. Mendelian randomization (MR) analyses integrated data from Finnish, Canadian, and UK Biobank cohorts to infer potential causal relationships.

Results

Recurrent GDM cases exhibited stage-specific microbial dysbiosis, characterized by an early enrichment of pro-inflammatory taxa (e.g., Streptococcus, Enterobacter) and a depletion of beneficial Bacteroidales, followed by a mid-pregnancy loss of Lactobacillaceae and Desulfovibrio. Network analysis identified reduced microbial community cohesion in recurrent GDM. Metabolomic profiling revealed a downregulation of caffeine metabolites and lipids in recurrent GDM. MR analyses suggested that 16 taxa may influence GDM risk, mediated by metabolites such as lipid signaling.

Conclusions

These findings highlight potential interventions for preventing GDM through modulation of gut microbiota and metabolic byproducts during pregnancy.

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来源期刊

Diabetes research and clinical practice 医学-内分泌学与代谢

CiteScore

10.30

自引率

3.90%

发文量

862

审稿时长

32 days

期刊介绍： Diabetes Research and Clinical Practice is an international journal for health-care providers and clinically oriented researchers that publishes high-quality original research articles and expert reviews in diabetes and related areas. The role of the journal is to provide a venue for dissemination of knowledge and discussion of topics related to diabetes clinical research and patient care. Topics of focus include translational science, genetics, immunology, nutrition, psychosocial research, epidemiology, prevention, socio-economic research, complications, new treatments, technologies and therapy.