探索凝结芽孢杆菌BCP92™在调节抑郁样行为中的作用：揭示微生物组肠-脑轴。

IF 2.3 3区心理学 Q2 BEHAVIORAL SCIENCES

Behavioural Brain Research Pub Date : 2025-08-12 DOI:10.1016/j.bbr.2025.115776

Sohel S. Shaikh , Kuldip Vyas , Ayush Sharma , Snehal S. Patel , Farhana Malek , Anis Malik

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引用次数: 0

摘要

背景：最近的研究表明，肠道微生物群可以显著影响认知健康。在本研究中，我们利用母体分离和慢性不可预测的轻度应激模型，探讨了凝固芽孢杆菌BCP92™对抑郁样行为的影响。方法：采用母鼠分离后慢性不可预测的轻度应激诱导大鼠抑郁。B. coagulansBCP92™以20亿CFU剂量给予大鼠6周。抑郁样行为采用蔗糖偏好测试、强迫游泳测试、高架加迷宫测试和野外测试进行评估。治疗期结束后，处死大鼠，采集脑、肠、粪标本进行生化、分子、组织病理学分析。结果：B凝血bcp92™减轻抑郁行为，行为评估的改善证明了这一点。治疗相关的神经递质如去甲肾上腺素、多巴胺、血清素和脑源性神经营养因子水平的变化，同时减少脑中的促炎标志物如c反应蛋白、TNF-α和IL-1β。此外，B. coagulansBCP92™给药恢复了脑内BDNF、双皮质素、GFAP和脑带闭塞的水平，表明具有神经保护作用。观察到，厚壁菌门与拟杆菌门的比例增加，粪便样本中的短链脂肪酸水平也较高。组织病理学分析表明，大脑和肠道结构都得到了保存，表明它们的完整性得到了维护。结论：本研究提示B. coagulansBCP92™通过调节肠-脑轴发挥抗抑郁作用，从而重塑肠道菌群，促进神经行为健康。

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Exploring role of Bacillus coagulans BCP92™ (MTCC 25460) in modulation of depressive-like behavior: Unraveling microbiome gut-brain axis

Background

Recent findings indicate that gut microbiota can significantly impact cognitive health. In this study, we explored the effects of Bacillus coagulans (Heyndrickxia coagulans) BCP92™ on depression-like behaviors using maternal separation and the chronic unpredictable mild stress model.

Methods

Depression in rats was induced through maternal separation followed by chronic unpredictable mild stress. B. coagulans BCP92™ was administered to the rats at a dose of 2 billion CFU for six weeks. Depression-like behaviors were assessed using the sucrose preference test, forced swim test, elevated plus maze test, and open-field test. After the treatment period, the animals were sacrificed, and brain, intestine, and fecal samples were collected for biochemical, molecular, and histopathological analyses.

Results

B coagulans BCP92™ mitigated depressive behaviors as evidenced by improvements in the behavior assessments. The treatment related changes in levels of neurotransmitters such as noradrenaline, dopamine, serotonin, and brain-derived neurotrophic factor while reducing pro-inflammatory markers like C-reactive protein, TNF-α, and IL-1β in the brain. Additionally, B. coagulans BCP92™ administration restored levels of BDNF, doublecortin, GFAP, and zona occludens in the brain, suggesting a neuroprotective effect. An increased ratio of Firmicutes to Bacteroidetes, along with higher levels of short-chain fatty acids in fecal samples, was observed. Histopathological analysis indicated that both brain and intestinal structures were preserved, demonstrating the maintenance of their integrity.

Conclusion

The findings from this study suggest that B. coagulans BCP92™ exerts antidepressant effects by modulating the gut-brain axis, thereby remodeling gut microbiota and promoting neurobehavioral health.

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来源期刊

Behavioural Brain Research 医学-行为科学

CiteScore

5.60

自引率

0.00%

发文量

383

审稿时长

61 days

期刊介绍： Behavioural Brain Research is an international, interdisciplinary journal dedicated to the publication of articles in the field of behavioural neuroscience, broadly defined. Contributions from the entire range of disciplines that comprise the neurosciences, behavioural sciences or cognitive sciences are appropriate, as long as the goal is to delineate the neural mechanisms underlying behaviour. Thus, studies may range from neurophysiological, neuroanatomical, neurochemical or neuropharmacological analysis of brain-behaviour relations, including the use of molecular genetic or behavioural genetic approaches, to studies that involve the use of brain imaging techniques, to neuroethological studies. Reports of original research, of major methodological advances, or of novel conceptual approaches are all encouraged. The journal will also consider critical reviews on selected topics.