通过转录组分析鉴定鸭肌内和皮下脂肪沉积的基因和lncrna。

IF 2.5 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Tingting Zhou, Xunhao Meng, Wenshuang Liang, Min Xue, Tianqi Yang, Yong Jiang, Hao Bai, Guobin Chang, Guohong Chen, Zhixiu Wang
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引用次数: 0

摘要

本研究旨在通过对肉鸭肌内前脂肪细胞(IMP-0)、肌内诱导4 d后脂肪细胞(IMP-4)、皮下前脂肪细胞(SCP-0)和皮下诱导4 d后脂肪细胞(SCP-4)的全转录组测序,鉴定参与肉鸭脂肪沉积的关键lncrna和mrna。我们在IMP-0-vs-IMP-4组中分别发现了1419个和697个差异表达基因和lncrna。在SCP-0-vs-SCP-4组中分别有2307个和1180个差异表达基因和差异lncrna。对IMP-0-vs-IMP-4和SCP-0-vs-SCP-4进行维恩图绘制,得到各组中CHKA、PNPLA2、PLPP1、FABP4、ACSL5、UGT8、FAT1和FADS2等独特的差异基因。在IMP-0-vs-IMP-4中,显著富集的GO项和KEGG通路是调控MAPK级联、脂质结合、花生四烯酸代谢等。在SCP-0-vs-SCP-4中,β -丙氨酸代谢、Wnt信号通路、脂质代谢过程等最为显著。通过相关性分析,IMP-0-vs-IMP-4组构建了193个节点和275条边的共表达网络,SCP-0-vs-SCP-4组构建了564个节点和3471条边的lncRNA-mRNA共表达网络。根据连接度筛选关键lncrna,包括MSTRG.8652.4、MSTRG.15586.1、MSTRG.6393.1、XR_217450.4、MSTRG.10341.22、XR_003492841.1、MSTRG.13937.5、XR_003493886.1。综上所述,目前的研究结果表明,在IMP-0-vs-IMP-4和SCP-0-vs-SCP-4中存在差异调控的差异基因、lncrna和富集途径。部分差异因子在肌内脂肪细胞诱导中表达量显著升高,而在皮下脂肪细胞诱导中表达量显著下调,如FABP3、MSTRG.13937.5、MSTRG.6393.1等。同时,也有一些因子被特异性调控,CHKA、PLA2G4A、FADS2、MSTRG.13842.1、MSTRG.16051.2、MSTRG.13842.1仅在皮下脂肪细胞中显著下调。这表明这些lncRNAs及其靶基因可能在肌内脂肪和皮下脂肪沉积中发挥重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification of genes and lncRNAs for intramuscular and subcutaneous fat deposition in ducks by transcriptome analysis.

The purpose of this study was to identify key lncRNAs and mRNAs involved in fat deposition of meat ducks based on whole transcriptome sequencing for intramuscular preadipocyte (IMP-0), intramuscular adipocyte after 4 days of induction (IMP-4), subcutaneous preadipocyte (SCP-0), and subcutaneous adipocyte after 4 days of induction (SCP-4). We found 1419 and 697 differentially expressed genes and lncRNAs in the IMP-0-vs-IMP-4 group, respectively. There were 2307 and 1180 differentially expressed genes and differential lncRNAs, respectively, in the SCP-0-vs-SCP-4 group. The IMP-0-vs-IMP-4 and SCP-0-vs-SCP-4 groups were plotted using a venn diagram to obtain unique differential genes in each group, such as CHKA, PNPLA2, PLPP1, FABP4, ACSL5, UGT8, FAT1 and FADS2. In IMP-0-vs-IMP-4, significant enriched GO terms and KEGG pathways were regulation of MAPK cascade, lipid binding, Arachidonic acid metabolism, etc. In SCP-0-vs-SCP-4, beta-alanine metabolism, Wnt signaling pathway, lipid metabolic process and so on were most significant . Based on correlation analysis, the IMP-0-vs-IMP-4 group constructes a co-expression network of 193 nodes and 275 edges, while the SCP-0-vs-SCP-4 group constitutes a lncRNA-mRNA co-expression network with 564 nodes and 3,471 edges. Key lncRNAs, including MSTRG.8652.4, MSTRG.15586.1, MSTRG.6393.1, XR_217450.4, MSTRG.10341.22, XR_003492841.1, MSTRG.13937.5, and XR_003493886.1 were screened according to connectivity degree. Taken together, the current findings indicated that there are differentially regulated differential genes, lncRNAs, and enrichment pathways in IMP-0-vs-IMP-4 and SCP-0-vs-SCP-4. Because of being differentially regulated, some differential factors were significantly increased in expression in intramuscular adipocyte induction while significantly downregulated in subcutaneous adipocyte induction, such as FABP3, MSTRG.13937.5, and MSTRG.6393.1. Meanwhile, there were also some factors that were specifically regulated, CHKA, PLA2G4A, FADS2, MSTRG.13842.1, MSTRG.16051.2 and MSTRG.13842.1 were significantly downregulated only in subcutaneous adipocytes. This suggests that these lncRNAs and their target genes may play important roles in intramuscular fat and subcutaneous fat deposition.

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来源期刊
Animal Bioscience
Animal Bioscience AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
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3 months
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