芦笋抗氧化和光保护活性物质的提取工艺优化

Q1 Environmental Science

Bioresource Technology Reports Pub Date : 2025-08-12 DOI:10.1016/j.biteb.2025.102253

Elif Toy , Beyza Tutku Bıçakcı , Cansu Erdem , Bahar Sincar , Feyza Özdemir , Melike Keskin , Dilek Yalçın , Gamze Turan , Alberto Baldelli , Oguz Bayraktar

{"title":"芦笋抗氧化和光保护活性物质的提取工艺优化","authors":"Elif Toy , Beyza Tutku Bıçakcı , Cansu Erdem , Bahar Sincar , Feyza Özdemir , Melike Keskin , Dilek Yalçın , Gamze Turan , Alberto Baldelli , Oguz Bayraktar","doi":"10.1016/j.biteb.2025.102253","DOIUrl":null,"url":null,"abstract":"<div><div>This study presents the development and optimization of a green extraction bioprocess for <em>Ulva rigida</em>, aiming to efficiently recover bioactive compounds with antioxidant and photoprotective properties using response surface methodology (RSM). A Box-Behnken design evaluated the combined effects of ethanol concentration (0–100 %), solid-to-liquid ratio (20–40 mL g<sup>−1</sup>), and extraction time (8–24 h) on extraction yield, antioxidant capacity, and sun protection factor (SPF). All variables significantly impacted extract quality, with ethanol concentration being the most influential. Optimal conditions (100 % ethanol, 1:20 ratio, 16 h) yielded an extract with SPF 11.61 and antioxidant activity of 16.91 mg Trolox/g. Preparative thin-layer chromatography (TLC) of the optimized extract produced three bioactive fractions exhibiting up to 2.3-fold higher SPF and 5-fold greater antioxidant activity. Liquid chromatography–mass spectrometry (LC-MS) analysis confirmed selective enrichment of carotenoids and polyphenols. These findings demonstrate the value of statistically optimized, bioactivity-guided extraction in enhancing <em>Ulva</em>-derived ingredients for cosmeceutical use.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"31 ","pages":"Article 102253"},"PeriodicalIF":0.0000,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Optimization of extraction for antioxidant and photoprotective bioactive compounds from Ulva rigida\",\"authors\":\"Elif Toy , Beyza Tutku Bıçakcı , Cansu Erdem , Bahar Sincar , Feyza Özdemir , Melike Keskin , Dilek Yalçın , Gamze Turan , Alberto Baldelli , Oguz Bayraktar\",\"doi\":\"10.1016/j.biteb.2025.102253\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>This study presents the development and optimization of a green extraction bioprocess for <em>Ulva rigida</em>, aiming to efficiently recover bioactive compounds with antioxidant and photoprotective properties using response surface methodology (RSM). A Box-Behnken design evaluated the combined effects of ethanol concentration (0–100 %), solid-to-liquid ratio (20–40 mL g<sup>−1</sup>), and extraction time (8–24 h) on extraction yield, antioxidant capacity, and sun protection factor (SPF). All variables significantly impacted extract quality, with ethanol concentration being the most influential. Optimal conditions (100 % ethanol, 1:20 ratio, 16 h) yielded an extract with SPF 11.61 and antioxidant activity of 16.91 mg Trolox/g. Preparative thin-layer chromatography (TLC) of the optimized extract produced three bioactive fractions exhibiting up to 2.3-fold higher SPF and 5-fold greater antioxidant activity. Liquid chromatography–mass spectrometry (LC-MS) analysis confirmed selective enrichment of carotenoids and polyphenols. These findings demonstrate the value of statistically optimized, bioactivity-guided extraction in enhancing <em>Ulva</em>-derived ingredients for cosmeceutical use.</div></div>\",\"PeriodicalId\":8947,\"journal\":{\"name\":\"Bioresource Technology Reports\",\"volume\":\"31 \",\"pages\":\"Article 102253\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-08-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioresource Technology Reports\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2589014X2500235X\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Environmental Science\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioresource Technology Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2589014X2500235X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Environmental Science","Score":null,"Total":0}

引用次数: 0

摘要

本研究旨在利用响应面法（response surface methodology， RSM）高效地提取具有抗氧化和光保护特性的生物活性化合物，并对绿色提取工艺进行了开发和优化。Box-Behnken设计评估了乙醇浓度（0 - 100%）、料液比（20-40 mL g−1）和提取时间（8-24 h）对提取率、抗氧化能力和防晒系数（SPF）的综合影响。各变量对提取物质量均有显著影响，其中乙醇浓度影响最大。最佳条件（100%乙醇，1:20比，16 h）得到的提取物SPF为11.61，抗氧化活性为16.91 mg Trolox/g。通过制备薄层色谱（TLC）对优化后的提取物进行分析，得到了三个生物活性组分，其SPF值提高了2.3倍，抗氧化活性提高了5倍。液相色谱-质谱（LC-MS）分析证实了类胡萝卜素和多酚的选择性富集。这些发现证明了统计优化、生物活性引导提取在提高ulva衍生成分药妆应用中的价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Optimization of extraction for antioxidant and photoprotective bioactive compounds from Ulva rigida

查看原文本刊更多论文

Optimization of extraction for antioxidant and photoprotective bioactive compounds from Ulva rigida

This study presents the development and optimization of a green extraction bioprocess for Ulva rigida, aiming to efficiently recover bioactive compounds with antioxidant and photoprotective properties using response surface methodology (RSM). A Box-Behnken design evaluated the combined effects of ethanol concentration (0–100 %), solid-to-liquid ratio (20–40 mL g⁻¹), and extraction time (8–24 h) on extraction yield, antioxidant capacity, and sun protection factor (SPF). All variables significantly impacted extract quality, with ethanol concentration being the most influential. Optimal conditions (100 % ethanol, 1:20 ratio, 16 h) yielded an extract with SPF 11.61 and antioxidant activity of 16.91 mg Trolox/g. Preparative thin-layer chromatography (TLC) of the optimized extract produced three bioactive fractions exhibiting up to 2.3-fold higher SPF and 5-fold greater antioxidant activity. Liquid chromatography–mass spectrometry (LC-MS) analysis confirmed selective enrichment of carotenoids and polyphenols. These findings demonstrate the value of statistically optimized, bioactivity-guided extraction in enhancing Ulva-derived ingredients for cosmeceutical use.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊