抑制蛋白激酶R可抑制HIV在CD4 T细胞中的复制和整合

IF 2 4区医学 Q2 IMMUNOLOGY

Journal of Virus Eradication Pub Date : 2025-08-07 DOI:10.1016/j.jve.2025.100605

Jaeden Pyburn , Juan Zhao , Ling Wang , Madison Schank , Addison C. Hill , Puja Banik , Yi Zhang , Xiao Y. Wu , Janet W. Lightner , Holly K. Orfield , Tabitha O. Leshaodo , Mohamed El Gazzar , Shunbin Ning , Jonathan P. Moorman , Zhi Q. Yao

{"title":"抑制蛋白激酶R可抑制HIV在CD4 T细胞中的复制和整合","authors":"Jaeden Pyburn , Juan Zhao , Ling Wang , Madison Schank , Addison C. Hill , Puja Banik , Yi Zhang , Xiao Y. Wu , Janet W. Lightner , Holly K. Orfield , Tabitha O. Leshaodo , Mohamed El Gazzar , Shunbin Ning , Jonathan P. Moorman , Zhi Q. Yao","doi":"10.1016/j.jve.2025.100605","DOIUrl":null,"url":null,"abstract":"<div><div>Evaluation of CD4 T cell status in early HIV infection is critical for developing strategies targeting HIV replication. In this study, we infected CD4 T cells with HIV-1 and investigated the cell survival mechanisms in HIV-infected versus uninfected cells during early HIV infection. Notably, HIV-infected CD4 T cells exhibited elevated levels of phosphorylated eukaryotic translation initiation factor 2-alpha (p-eIF2α) compared to uninfected cells. Importantly, inhibition of protein kinase R (PKR) in HIV-infected cells significantly suppressed HIV p24 protein expression by disrupting HIV reverse transcription and integration. These results suggest that targeting PKR could be a promising therapeutic approach against HIV infection.</div></div>","PeriodicalId":17552,"journal":{"name":"Journal of Virus Eradication","volume":"11 3","pages":"Article 100605"},"PeriodicalIF":2.0000,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Inhibition of protein kinase R suppresses HIV replication and integration in CD4 T cells\",\"authors\":\"Jaeden Pyburn , Juan Zhao , Ling Wang , Madison Schank , Addison C. Hill , Puja Banik , Yi Zhang , Xiao Y. Wu , Janet W. Lightner , Holly K. Orfield , Tabitha O. Leshaodo , Mohamed El Gazzar , Shunbin Ning , Jonathan P. Moorman , Zhi Q. Yao\",\"doi\":\"10.1016/j.jve.2025.100605\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Evaluation of CD4 T cell status in early HIV infection is critical for developing strategies targeting HIV replication. In this study, we infected CD4 T cells with HIV-1 and investigated the cell survival mechanisms in HIV-infected versus uninfected cells during early HIV infection. Notably, HIV-infected CD4 T cells exhibited elevated levels of phosphorylated eukaryotic translation initiation factor 2-alpha (p-eIF2α) compared to uninfected cells. Importantly, inhibition of protein kinase R (PKR) in HIV-infected cells significantly suppressed HIV p24 protein expression by disrupting HIV reverse transcription and integration. These results suggest that targeting PKR could be a promising therapeutic approach against HIV infection.</div></div>\",\"PeriodicalId\":17552,\"journal\":{\"name\":\"Journal of Virus Eradication\",\"volume\":\"11 3\",\"pages\":\"Article 100605\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-08-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Virus Eradication\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S205566402500024X\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Virus Eradication","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S205566402500024X","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"IMMUNOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

评估CD4 T细胞在早期HIV感染中的状态对于制定针对HIV复制的策略至关重要。在这项研究中，我们用HIV-1感染CD4 T细胞，并研究了HIV感染和未感染细胞在HIV感染早期的细胞存活机制。值得注意的是，与未感染的细胞相比，hiv感染的CD4 T细胞表现出磷酸化的真核翻译起始因子2- α (p-eIF2α)水平升高。重要的是，抑制HIV感染细胞中的蛋白激酶R （PKR）通过破坏HIV逆转录和整合显著抑制HIV p24蛋白的表达。这些结果表明，靶向PKR可能是一种有希望的治疗HIV感染的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Inhibition of protein kinase R suppresses HIV replication and integration in CD4 T cells

Evaluation of CD4 T cell status in early HIV infection is critical for developing strategies targeting HIV replication. In this study, we infected CD4 T cells with HIV-1 and investigated the cell survival mechanisms in HIV-infected versus uninfected cells during early HIV infection. Notably, HIV-infected CD4 T cells exhibited elevated levels of phosphorylated eukaryotic translation initiation factor 2-alpha (p-eIF2α) compared to uninfected cells. Importantly, inhibition of protein kinase R (PKR) in HIV-infected cells significantly suppressed HIV p24 protein expression by disrupting HIV reverse transcription and integration. These results suggest that targeting PKR could be a promising therapeutic approach against HIV infection.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Journal of Virus Eradication Medicine-Public Health, Environmental and Occupational Health

CiteScore

6.10

自引率

1.80%

发文量

审稿时长

39 weeks

期刊介绍： The Journal of Virus Eradication aims to provide a specialist, open-access forum to publish work in the rapidly developing field of virus eradication. The Journal covers all human viruses, in the context of new therapeutic strategies, as well as societal eradication of viral infections with preventive interventions. The Journal is aimed at the international community involved in the prevention and management of viral infections. It provides an academic forum for the publication of original research into viral reservoirs, viral persistence and virus eradication and ultimately development of cures. The Journal not only publishes original research, but provides an opportunity for opinions, reviews, case studies and comments on the published literature. It focusses on evidence-based medicine as the major thrust in the successful management of viral infections.The Journal encompasses virological, immunological, epidemiological, modelling, pharmacological, pre-clinical and in vitro, as well as clinical, data including but not limited to drugs, immunotherapy and gene therapy. It is an important source of information on the development of vaccine programs and preventative measures aimed at virus eradication.