丁酸和丙酸通过激活g蛋白偶联受体41对人牙周膜细胞的协同促炎作用。

IF 3.2 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE
Xianjun Lu, Zijian Yuan, Wenling Huang, Jingjing Liu, Baoqi Chen, Lihong Guo
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引用次数: 0

摘要

目的:牙周炎是一种慢性疾病,最终会导致牙齿脱落。本研究旨在探讨短链脂肪酸(SCFAs)对人牙周韧带细胞(hPDLCs)的炎症作用。方法:采用免疫荧光法检测G蛋白偶联受体(GPRs)在hpdlc中的表达。利用细胞计数试剂盒-8和酶联免疫吸附测定试剂盒检测SCFAs对hpdlc中促炎因子的增殖和分泌的调节作用。采用Western blot分析研究SCFAs诱导hpdlc的潜在炎症反应。结果:GPR41在hpdlc表面表达。暴露于7 mM丁酸和10 mM丙酸后,hpdlc的活力显著降低。此外,10 mM丁酸和10 mM丙酸联合使用可显著增加hpdlc中白细胞介素(IL)-6、IL-1β和肿瘤坏死因子-α的产生。7 mM丁酸和10 mM丙酸处理导致hpdlc中p65磷酸化显著增加。与磷酸盐缓冲盐水对照组相比,加入GPR41受体抑制剂后的增殖和促炎作用无统计学差异。结论:丁酸和丙酸在抑制hpdlc增殖和增强其促炎反应方面具有协同作用。这可能是通过激活GPR41介导的。此外,这些酸可以协同激活hpdlc中通过GPR41的核因子-κB信号通路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The synergistic pro-inflammatory effect of butyric and propionic acid on human periodontal ligament cells via activation of G-protein-coupled receptor 41.

Purpose: Periodontitis is a chronic disease that ultimately leads to tooth loss. This study aimed to investigate the inflammatory effects of short-chain fatty acids (SCFAs) on human periodontal ligament cells (hPDLCs).

Methods: Immunofluorescence was employed to assess the expression of G protein-coupled receptors (GPRs) in hPDLCs. The Cell Counting Kit-8 and enzyme-linked immunosorbent assay kits were utilized to examine the regulatory effects of SCFAs on both the proliferation and secretion of pro-inflammatory factors in hPDLCs. Western blot analysis was performed to investigate the potential inflammatory responses in hPDLCs induced by SCFAs.

Results: GPR41 was expressed on the surface of hPDLCs. The viability of hPDLCs was significantly reduced after exposure to both 7 mM butyric acid and 10 mM propionic acid. Additionally, a combination of 10 mM butyric acid and 10 mM propionic acid significantly increased the production of interleukin (IL)-6, IL-1β, and tumor necrosis factor-α in hPDLCs. Treatment with both 7 mM butyric acid and 10 mM propionic acid led to a significant increase in p65 phosphorylation in hPDLCs. There was no statistical difference in proliferation and pro-inflammatory effects after the addition of a GPR41 receptor inhibitor compared to the phosphate-buffered saline control.

Conclusions: Butyric acid and propionic acid exhibited a synergistic effect in inhibiting the proliferation of hPDLCs and enhancing their pro-inflammatory responses. This may be mediated through the activation of GPR41. Additionally, these acids could synergistically activate the nuclear factor-κB signaling pathway via GPR41 in hPDLCs.

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来源期刊
Journal of Periodontal and Implant Science
Journal of Periodontal and Implant Science DENTISTRY, ORAL SURGERY & MEDICINE-
CiteScore
3.30
自引率
5.30%
发文量
38
期刊介绍: Journal of Periodontal & Implant Science (JPIS) is a peer-reviewed and open-access journal providing up-to-date information relevant to professionalism of periodontology and dental implantology. JPIS is dedicated to global and extensive publication which includes evidence-based original articles, and fundamental reviews in order to cover a variety of interests in the field of periodontal as well as implant science.
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