CHAF1A促进前脂肪细胞分化并参与妊娠糖尿病的巨大儿。

IF 2.5 3区 医学 Q2 OBSTETRICS & GYNECOLOGY
Reproductive Sciences Pub Date : 2025-09-01 Epub Date: 2025-08-12 DOI:10.1007/s43032-025-01946-z
Dandan Xia, Xun Xu, Yuhui Zhang, Chenying Zhang, Huiyan Wang
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引用次数: 0

摘要

妊娠期糖尿病(GDM)导致巨大儿的主要原因是脂肪细胞分化引起的脂肪堆积。本研究旨在探讨染色质组装因子1亚单位A (CHAF1A)在gdm诱导的巨大儿中的作用及其潜在机制。比较GDM伴巨大儿组(n = 25)与正常血糖伴正常体重组(n = 15) CHAF1A的表达,并检测CHAF1A与新生儿体成分的相关性。CHAF1A在人内脏前脂肪细胞(HPA-v)中过表达和敲低,测定其对细胞增殖和成脂分化的影响,然后测定成脂标志物的表达。利用转录组测序来研究潜在的机制。胎盘免疫组化结果显示,CHAF1A在GDM伴巨大儿组的表达显著高于对照组(P
本文章由计算机程序翻译,如有差异,请以英文原文为准。
CHAF1A Promotes Preadipocyte Differentiation and Contributes to Macrosomia in Gestational Diabetes Mellitus.

Gestational diabetes mellitus (GDM) leads to macrosomia primarily due to fat accumulation caused by adipocyte differentiation. This study aims to investigate the role and underlying mechanisms of Chromatin assembly factor 1 subunit A (CHAF1A) in GDM-induced macrosomia. CHAF1A expression was compared between the GDM with macrosomia group (n = 25) and the normal glucose with normal weight group (n = 15), and the correlation between CHAF1A and neonatal body composition was examined. CHAF1A was overexpressed and knocked down in human visceral preadipocytes (HPA-v), and the effects on cell proliferation and adipogenic differentiation were measured, then the expressions of adipogenic markers were determined. Transcriptome sequencing was employed to investigate the potential mechanisms. Placental immunohistochemistry showed that the expression of CHAF1A in the GDM with macrosomia group was significantly higher than that in the control group (P < 0.05). Correlation analysis showed that CHAF1A expression was positively correlated with neonatal weight, body fat percentage, and fat mass. In functional assays, preadipocytes overexpressing CHAF1A showed enhanced proliferation and adipogenic differentiation, while knockdown of CHAF1A resulted in the opposite effect. Moreover, CHAF1A affected the expression of adipogenic markers. Transcriptome sequencing analysis showed that the differentially expressed genes after CHAF1A silencing were enriched in signaling pathways closely related to preadipocyte differentiation and hormone secretion and synthesis, such as JAK-STAT, Wnt and BMP signaling pathways. CHAF1A promotes the proliferation and differentiation of preadipocytes, which may be a direction for exploring fetal fat accumulation leading to macrosomia in GDM.

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来源期刊
Reproductive Sciences
Reproductive Sciences 医学-妇产科学
CiteScore
5.50
自引率
3.40%
发文量
322
审稿时长
4-8 weeks
期刊介绍: Reproductive Sciences (RS) is a peer-reviewed, monthly journal publishing original research and reviews in obstetrics and gynecology. RS is multi-disciplinary and includes research in basic reproductive biology and medicine, maternal-fetal medicine, obstetrics, gynecology, reproductive endocrinology, urogynecology, fertility/infertility, embryology, gynecologic/reproductive oncology, developmental biology, stem cell research, molecular/cellular biology and other related fields.
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