肉鸡饲养过程中鸡窝细菌群落的时间变化:基于16S rRNA的宏基因组研究

IF 3.2 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Embar Prasanna Kannan, Prasanth Venkatachalam, Judy Gopal, Ponmariappan Sarkaraisamy, Manikandan Muthu
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引用次数: 0

摘要

目的:在印度泰米尔纳德邦马拉卡南市的一个商业肉鸡养殖场,在六周的饲养周期内,破译家禽产仔中不同时间框架的细菌群落变化。方法与结果:采用基于16S rRNA的宏基因组牛津纳米孔测序法对禽窝菌群进行鉴定,并采用kraken2工具进行分类。我们的研究结果揭示了家禽窝中细菌的不同优势模式(P1-P6), P1中占优势的是鞘杆菌sp. 21 (53%), P2中占优势(~ 12%),P3中占优势的是海洋单胞菌sp. gk1 (~ 14%), P6中占优势的是>50%),P4中占优势的是无氧球菌(~ 10%),P5中占优势的是烃类海洋杆菌(~ 15%)。重要的是,在关键的生物地球化学循环中检测到各种细菌,包括富营养化亚硝酸单胞菌(氮循环)、枯草芽孢杆菌(磷循环)和钙酸硫杆菌(硫循环),这表明利用家禽粪便作为有效的生物肥料具有很强的潜力。另一方面,还发现了包括结核分枝杆菌、大肠杆菌、肠炎沙门氏菌和肺炎链球菌在内的有害人类病原体,这表明将家禽粪便用作肥料与严重的公共卫生问题有关。结论:本研究提供的知识既可以利用家禽垃圾作为生物肥料的有用性,也可以减轻家禽垃圾携带的有害病原体的传播。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Temporal shift of bacterial communities in poultry litter during the course of broiler chicken rearing: a 16S rRNA-based metagenomic study.

Aim: To decipher the bacterial community transitions of poultry litter at various time frames over a 6-week rearing cycle in a commercial broiler chicken poultry farm in Marakanam, Tamil Nadu, India.

Methods and results: The bacterial consortia of poultry litter were elucidated using the 16S rRNA-based metagenomic Oxford nanopore sequencing method, followed by taxonomic assignment using the Kraken2 tool. Our findings unveiled the varied dominance patterns of bacteria in poultry litter (P1-P6) with Sphingobacterium sp. 21 (53%) in P1, Amphibacillus xylanus dominated in P2 (∼12%), Oceanimonas sp. GK1 in P3 (∼14%) and P6 (>50%), Anaerococcus prevotii in P4 (∼10%) and Marinobacter hydrocarbonoclasticus (∼15%) in P5. Importantly various bacteria involved in key biogeochemical cycles, including Nitrosomonas eutropha (nitrogen cycle), Bacillus subtilis (phosphorous cycle), and Acidithiobacillus caldus (sulphur cycle) were detected indicating a strong potential of utilizing the poultry litter as an effective biofertilizer. On the other hand, harmful human pathogens, including Mycobacterium tuberculosis, Escherichia coli, Salmonella enterica, and Streptococcus pneumoniae were also detected indicating significant public health concern associated with the application of poultry litter as fertilizer.

Conclusions: This study offers knowledge to both leverage the usefulness of poultry litter as biofertilizer and to mitigate transmission of harmful pathogens harboured by poultry litter.

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来源期刊
Journal of Applied Microbiology
Journal of Applied Microbiology 生物-生物工程与应用微生物
CiteScore
7.30
自引率
2.50%
发文量
427
审稿时长
2.7 months
期刊介绍: Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.
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