胃针插入的力学生物学:实验与数值结合的研究。

IF 2.7 3区 医学 Q2 BIOPHYSICS
Sif Julie Friis, Torben Strøm Hansen, Mette Poulsen, Peter Helding Kvist, Ansgar Petersen, Hans Gregersen, Jens Vinge Nygaard
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引用次数: 0

摘要

生物药物的使用增加了对替代胃给药方法的需求。设计将药物插入粘膜衬里的装置的开始克服了传统给药方法的局限性。来自这种微针插入的机械力可以影响组织和细胞行为,特别是机械转导标记物。本研究探讨了针插入胃组织的影响,为设计替代给药装置提供信息。采用实验和计算相结合的方法,对猪胃组织进行拉伸和径向压缩试验,建立有限元分析模型。该模型通过基于原子力显微镜(AFM)的微压痕来验证,以检查插入部位附近的刚度变化,并分析yes-相关蛋白1 (YAP-1)的表达以评估细胞力学转导。AFM结果显示,与插入部位距离相关的组织刚度降低(p
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mechanobiology of gastric needle insertions: a combined experimental and numerical study

The rising use of biologic drugs has increased the demand for alternative gastric administration methods. Inception of devices engineered to insert medication into the mucosal lining overcomes limitations of traditional administration methods. Mechanical forces from such microneedle insertions can affect tissue and cellular behavior, particularly mechanotransduction markers. This study investigates the effects of needle insertion in gastric tissue to inform the design of alternative drug delivery devices. Experimental and computational approaches were utilized, using tension and radial compression tests on porcine gastric tissue to inform a finite element analysis (FEA) model. This model was validated with atomic force microscopy (AFM)-based micro-indentation to examine stiffness variations near the insertion site, and yes-associated-protein-1 (YAP-1) expression was analyzed to assess cellular mechanotransduction. AFM results revealed a distance-dependent decrease in tissue stiffness from the insertion site (p < 0.05), with significant differences in needle geometry (p < 0.05). The FEA model correlated well with AFM findings, confirming its validity for further cellular simulations. Mechanical stresses from needle insertion were shown to propagate through the tissue, affecting both cytoplasmic and nuclear stress distributions and altering nuclear morphology near the insertion site. The blunt needle produced a higher localized stress field compared to the sharp needle. Additionally, YAP-1 expression was lower in the injected samples than in control samples showing distance-dependent responses observed. This study demonstrates a validated model linking tissue mechanics and cellular responses, highlighting how needle geometry impacts gastric tissue mechanics and mechanotransduction, providing insights essential for designing gastric drug delivery devices.

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来源期刊
Biomechanics and Modeling in Mechanobiology
Biomechanics and Modeling in Mechanobiology 工程技术-工程:生物医学
CiteScore
7.10
自引率
8.60%
发文量
119
审稿时长
6 months
期刊介绍: Mechanics regulates biological processes at the molecular, cellular, tissue, organ, and organism levels. A goal of this journal is to promote basic and applied research that integrates the expanding knowledge-bases in the allied fields of biomechanics and mechanobiology. Approaches may be experimental, theoretical, or computational; they may address phenomena at the nano, micro, or macrolevels. Of particular interest are investigations that (1) quantify the mechanical environment in which cells and matrix function in health, disease, or injury, (2) identify and quantify mechanosensitive responses and their mechanisms, (3) detail inter-relations between mechanics and biological processes such as growth, remodeling, adaptation, and repair, and (4) report discoveries that advance therapeutic and diagnostic procedures. Especially encouraged are analytical and computational models based on solid mechanics, fluid mechanics, or thermomechanics, and their interactions; also encouraged are reports of new experimental methods that expand measurement capabilities and new mathematical methods that facilitate analysis.
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