甘草素通过激活ERK/JNK通路和提供抗氧化保护,对骨髓间充质干细胞成骨分化和骨折愈合具有双重作用

IF 2.6 4区 医学 Q2 PHARMACOLOGY & PHARMACY
Haijun Mao, Jing Liu, Juan Feng, Yalikun Yusufu, Lei Yang, Qing Jiang, Minghui Sun
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引用次数: 0

摘要

甘草素是甘草中的一种黄酮类化合物,具有多种药理特性,但其对骨折愈合的影响尚不清楚。本研究探讨了其体外对骨髓间充质干细胞(BMSCs)的成骨和抗氧化作用及其对大鼠股骨骨折的体内再生作用。细胞毒性通过MTT测定,成骨分化通过ALP和ARS染色。大鼠股骨骨折模型验证了其治疗效果,并通过显微ct和组织学评估评估愈合效果。通过RT-qPCR、western blot和免疫荧光分析成骨和信号通路中基因和蛋白的表达。采用DCFH-DA染色法测定ROS水平。结果表明,利尿素可促进骨髓间充质干细胞成骨,其表现为ALP活性和钙沉积的增加。在大鼠中,它促进骨再生并上调磷酸化的ERK和JNK。此外,它还能减少氧化损伤后ROS的产生,促进NRF2、HO1和NQO1的表达。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Liquiritin Exerts a Dual Effect on BMSCs Osteogenic Differentiation and Fracture Healing by Activating the ERK/JNK Pathway and Providing Antioxidant Protection

Liquiritin Exerts a Dual Effect on BMSCs Osteogenic Differentiation and Fracture Healing by Activating the ERK/JNK Pathway and Providing Antioxidant Protection

Liquiritin Exerts a Dual Effect on BMSCs Osteogenic Differentiation and Fracture Healing by Activating the ERK/JNK Pathway and Providing Antioxidant Protection

Liquiritin Exerts a Dual Effect on BMSCs Osteogenic Differentiation and Fracture Healing by Activating the ERK/JNK Pathway and Providing Antioxidant Protection

Liquiritin Exerts a Dual Effect on BMSCs Osteogenic Differentiation and Fracture Healing by Activating the ERK/JNK Pathway and Providing Antioxidant Protection

Liquiritin, a flavonoid from Glycyrrhiza uralensis L., has diverse pharmacological properties, but its impact on fracture healing is unexplored. This study investigates its osteogenic and antioxidative effects on bone marrow mesenchymal stem cells (BMSCs) in vitro and its regenerative effect on rat femoral fractures in vivo. Cytotoxicity is assessed via MTT assay, and osteogenic differentiation via ALP and ARS staining. A rat femoral fracture model validates its therapeutic effects, with micro-CT and histological assessments evaluating healing outcomes. Gene and protein expression in osteogenic and signaling pathways are analyzed through RT-qPCR, western blot, and immunofluorescence. ROS levels are measured using DCFH-DA staining. Results show liquiritin enhances osteogenesis in BMSCs, evidenced by increased ALP activity and calcium deposition. In rats, it facilitates bone regeneration and upregulates phosphorylated ERK and JNK. Additionally, it reduces ROS generation post-oxidative injury, boosting NRF2, HO1, and NQO1 expression.

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来源期刊
Advanced Therapeutics
Advanced Therapeutics Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
7.10
自引率
2.20%
发文量
130
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