间充质干细胞细胞外小泡规模化生产的一步冷冻干燥策略

IF 2.6 4区 医学 Q2 PHARMACOLOGY & PHARMACY
Yashvi Sharma, Meenakshi Mendiratta, Suchi Gupta, Sonali Rawat, Pardeep Kumar Vaishnav, Sujata Mohanty
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引用次数: 0

摘要

间充质干细胞衍生的小细胞外囊泡(MSC-sEV)由于其再生和免疫调节特性而具有巨大的应用前景。然而,它们的广泛应用受到储存、稳定性和冷链运输方面的挑战的阻碍。在本研究中,研究人员探索了冻干作为延长MSC-sEV保质期的策略,同时保持其结构完整性和生物功能。冻干的sEV储存在四种不同的温度下-室温(RT), 4, - 20°C和- 80°C -持续时间为1,3和6个月。研究结果表明,4°C的正常冷藏温度可以保持sEV稳定性长达1个月,而- 20°C和- 80°C的冷藏温度更有效,可以保持sEV完整性和功能6个月甚至更长时间。这些结果强调了优化冻干MSC-sEV储存方案的重要性,以确保其临床和研究应用的可行性。本研究为改善MSC-sEV的储存和冷链运输奠定了基础,为其整合到可扩展和标准化的治疗平台铺平了道路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

One-Step Freeze Drying Strategy for Scalable Production of Small Extracellular Vesicles Derived From Mesenchymal Stem Cells

One-Step Freeze Drying Strategy for Scalable Production of Small Extracellular Vesicles Derived From Mesenchymal Stem Cells

One-Step Freeze Drying Strategy for Scalable Production of Small Extracellular Vesicles Derived From Mesenchymal Stem Cells

One-Step Freeze Drying Strategy for Scalable Production of Small Extracellular Vesicles Derived From Mesenchymal Stem Cells

One-Step Freeze Drying Strategy for Scalable Production of Small Extracellular Vesicles Derived From Mesenchymal Stem Cells

The clinical translation of mesenchymal stem cell-derived small extracellular vesicles (MSC-sEV) holds immense promise due to their regenerative and immunomodulatory properties. However, their widespread application is hindered by challenges in storage, stability, and cold chain transport. In this study, it is explored lyophilisation as a strategy to extend the shelf life of MSC-sEV while maintaining their structural integrity and biological functionality. Lyophilised sEV are stored at four different temperatures—room temperature (RT), 4, −20°C, and −80 °C—for durations of 1, 3, and 6 months. This findings reveal that normal refrigeration temperature of 4 °C storage is suitable for maintaining sEV stability for up to 1 month, while −20°C and −80 °C are more effective for longer durations, preserving sEV integrity and functionality for 6 months and beyond. These results underscore the importance of optimizing storage protocols for lyophilised MSC-sEV to ensure their viability for clinical and research applications. This study establishes a foundation for improved storage and cold chain transport of MSC-sEV, paving the way for their integration into scalable and standardized therapeutic platforms.

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来源期刊
Advanced Therapeutics
Advanced Therapeutics Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
7.10
自引率
2.20%
发文量
130
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