从携带抗肌营养不良蛋白基因c.3682delG突变的患者外周血单核细胞中生成人诱导多能干细胞系（LZUSHi003-A）

IF 0.7 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-08-09 DOI:10.1016/j.scr.2025.103800

Jie Shi , Jiao Yang , Huijun Gao , Lan Yang , Yinpeng Zhang , Ansheng Zhao , Rui Yang , Zhonghong Xiong , Jing Yang , Bei Gao

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引用次数: 0

摘要

杜氏肌营养不良症（DMD）以进行性肌肉变性为特征，是由肌营养不良蛋白基因突变引起的。在这里，我们使用仙台病毒从一名携带DMD基因突变c.3682delG的13岁男性患者的外周单核细胞中产生人诱导多能干细胞系LZUSHi003-A。LZUSHi003-A细胞系在表达多能性标记物的同时保留了这种突变。此外，它还表现出正常的核型，并能分化成三个胚胎胚层。

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Generation of a human induced pluripotent stem cell line (LZUSHi003-A) from peripheral mononuclear cells of a patient carrying the c.3682delG mutation in the dystrophin gene

Duchenne muscular dystrophy (DMD) is characterized by progressive muscle degeneration and is caused by mutations in the dystrophin gene. Here, we used Sendai virus to generate a human induced pluripotent stem cell line, LZUSHi003-A, from the peripheral mononuclear cells of a 13-year-old male patient carrying the DMD gene mutation c.3682delG. The LZUSHi003-A cell line retained this mutation, while expressing pluripotency markers. Additionally, it demonstrated a normal karyotype and could differentiate into three embryonic germ layers.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.