含米壳液烟口腔薄膜对牙周炎模型中关键细胞因子和生长因子表达的影响

Q1 Medicine

Journal of oral biology and craniofacial research Pub Date : 2025-08-13 DOI:10.1016/j.jobcr.2025.08.007

Gustiadi Saputra , Ira Arundina , Theresia Indah Budhy , Dieni Mansur , Meircurius Dwi Condro Surboyo , Cheng Hwee Ming , Sheryn Marcha Ramaniasari , Azzahra Salsabila Adira Moelyanto

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引用次数: 0

摘要

背景：牙龈卟啉单胞菌是引起牙周炎的主要病原体，可引起炎症和牙槽骨丢失。细胞因子和生长因子的失衡导致疾病进展。稻壳液烟具有潜在的抗菌和抗炎作用。目的制备含谷壳液烟口腔薄膜并对其进行表征，并评价其在牙周炎模型中的抗菌活性和生物学效应。方法以羟丙基甲基纤维素、明胶、山梨醇和稻壳液烟为原料，采用溶剂铸造法制备sotfs。测定其重量、厚度、溶胀、崩解、溶出度以及对牙龈卟啉单胞菌的抗菌活性。在体内实验中，28只Wistar大鼠被诱导牙周炎。应用于牙周袋。在第2天和第7天收集下颌组织，免疫组化分析IL-6、IL-10、FGF-2和BMP-2的表达。结果制备的OTFs具有重量轻、厚度适中、崩解和溶出时间快等良好的物理性能(p <；0.05)。OTF和空白膜均能抑制牙龈卟啉单胞菌的生长。OTF治疗显著降低了两个时间点的IL-6，增加了IL-10、FGF-2和BMP-2的表达(p <；0.05)，表明炎症减轻，组织再生增强。结论稻壳液烟OTF具有较强的抗菌和免疫调节作用，有望成为治疗牙周炎的新策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Oral thin film containing rice husk liquid smoke effects on the expression of key cytokines and growth factors in a periodontitis model

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Oral thin film containing rice husk liquid smoke effects on the expression of key cytokines and growth factors in a periodontitis model

Background

Porphyromonas gingivalis is a key pathogen in periodontitis, which involves inflammation and alveolar bone loss. An imbalance of cytokines and growth factors contributes to disease progression. Rice husk liquid smoke has shown potential antibacterial and anti-inflammatory properties.

Objective

This study aimed to formulate and characterize an oral thin film (OTF) containing rice husk liquid smoke and evaluate its antibacterial activity and biological effects in a periodontitis model.

Methods

OTFs were prepared using hydroxypropyl methylcellulose, gelatin, sorbitol, and rice husk liquid smoke via solvent casting. The OTF were characterized for weight, thickness, swelling, disintegration, dissolution, and antibacterial activity against Porphyromonas gingivalis. For in vivo testing, periodontitis was induced in 28 Wistar rats. OTFs were applied to periodontal pockets. Mandibular tissues were collected on days 2 and 7 for immunohistochemical analysis of IL-6, IL-10, FGF-2, and BMP-2 expression.

Results

The OTFs showed favorable physical properties, including low weight, appropriate thickness, and faster disintegration and dissolution times (p < 0.05). Both OTF and blank films inhibited Porphyromonas gingivalis growth. OTF treatment significantly decreased IL-6 and increased IL-10, FGF-2, and BMP-2 expression at both time points (p < 0.05), indicating reduced inflammation and enhanced tissue regeneration.

Conclusion

The OTF containing rice husk liquid smoke exhibited strong antibacterial and immunomodulatory effects, suggesting its potential as a novel therapeutic strategy for periodontitis.

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来源期刊

Journal of oral biology and craniofacial research Medicine-Otorhinolaryngology

CiteScore

4.90

自引率

0.00%

发文量

133

审稿时长

167 days

期刊介绍： Journal of Oral Biology and Craniofacial Research (JOBCR)is the official journal of the Craniofacial Research Foundation (CRF). The journal aims to provide a common platform for both clinical and translational research and to promote interdisciplinary sciences in craniofacial region. JOBCR publishes content that includes diseases, injuries and defects in the head, neck, face, jaws and the hard and soft tissues of the mouth and jaws and face region; diagnosis and medical management of diseases specific to the orofacial tissues and of oral manifestations of systemic diseases; studies on identifying populations at risk of oral disease or in need of specific care, and comparing regional, environmental, social, and access similarities and differences in dental care between populations; diseases of the mouth and related structures like salivary glands, temporomandibular joints, facial muscles and perioral skin; biomedical engineering, tissue engineering and stem cells. The journal publishes reviews, commentaries, peer-reviewed original research articles, short communication, and case reports.