MiR-182-5p通过靶向SIRT1促进结直肠癌细胞的增殖、迁移和侵袭

IF 2.5 4区综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES

Journal of Radiation Research and Applied Sciences Pub Date : 2025-08-12 DOI:10.1016/j.jrras.2025.101850

Hongwei Fan , Yingxin Wang , Shuqiao Zhao , Rong Ai , Chao Dong

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引用次数: 0

摘要

结直肠癌（colorectal cancer， CRC）是恶性程度最高的恶性肿瘤之一。MicroRNAs （miRNA）是参与许多生理或病理过程的小的非编码rna。microRNA-182-5p （miR-182-5p）的异常表达已在多种癌症中发现。但miR-182-5p在结直肠癌中的表达谱和生物学功能存在争议。方法采用实时荧光定量PCR （quantitative real-time PCR, qPCR）检测miR-182-5p水平，采用log-rank检验检测CRC患者生存率的差异。采用集落形成法检测结直肠癌细胞的增殖，采用划痕法和transwell法检测结直肠癌细胞的迁移和侵袭。我们通过双荧光素酶报告基因（DLR）检测确定了miR-182-5p对sirtuin 1 （SIRT1）的调控作用。结果我们的研究表明，miR-182-5p水平在结直肠癌中显著升高。对于结直肠癌患者的总生存期（OS）， miR-182-5p水平可作为预后的独立预测因子。此外，miR-182-5p促进CRC细胞的增殖、迁移和侵袭。在机制上，miR-182-5p靶向SIRT1介导其在CRC中的致癌作用。因此，本研究结果表明，miR-182-5p水平升高是CRC患者OS的独立预后因素，miR-182-5p通过靶向SIRT1增强CRC细胞的增殖、迁移和侵袭。这些结果可能为结直肠癌提供诊断标志和治疗靶点。

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MiR-182-5p propels colorectal cancer cell proliferation, migration and invasion via targeting SIRT1

Background

Colorectal carcinoma (CRC) is ranked among the most malignant malignancies. MicroRNAs (miRNA) are small non-coding RNAs involved in numerous physiological or pathological processes. Abnormal microRNA-182-5p (miR-182-5p) expression has been indicated in various carcinomas. But expression profile and biology functions of miR-182-5p in CRC is controversial.

Methods

Here, we detected miR-182-5p level utilizing quantitative real-time PCR (qPCR), and the differences in CRC patients’ survival rates were measured utilizing log-rank test. We detected CRC cell proliferation utilizing Colony formation assay, while CRC cell migration and invasion utilizing scratch assay and transwell assay. We identified regulatory effect of miR-182-5p on sirtuin 1 (SIRT1) via Dual-luciferase reporter (DLR) assay.

Results

Our research indicates that miR-182-5p level is dramatically increased in CRC. For overall survival (OS) of CRC patients, miR-182-5p level acts as an independent predictor of prognosis. Moreover, miR-182-5p facilitates CRC cell proliferation, migration and invasion. Mechanistically, miR-182-5p targets SIRT1 to mediate its oncogenic role in CRC.

Conclusion

Therefore, the present results reveal that elevated miR-182-5p level is an independent prognostic factor for CRC patients’ OS, and miR-182-5p enhances CRC cell proliferation, migration and invasion via targeting SIRT1. Such outcomes might provide CRC with diagnostic marker and treatment target point.

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来源期刊

Journal of Radiation Research and Applied Sciences MULTIDISCIPLINARY SCIENCES-

自引率

5.90%

发文量

130

审稿时长

16 weeks

期刊介绍： Journal of Radiation Research and Applied Sciences provides a high quality medium for the publication of substantial, original and scientific and technological papers on the development and applications of nuclear, radiation and isotopes in biology, medicine, drugs, biochemistry, microbiology, agriculture, entomology, food technology, chemistry, physics, solid states, engineering, environmental and applied sciences.