将炎症与基因表达联系起来：来自强直性脊柱炎的GPR65、NUAK2和OPG的见解

IF 0.9 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2025-08-09 DOI:10.1016/j.genrep.2025.102315

Safa Alshattawi , Rand Moushtaq Taleb , Ghanyia Jasim shanyoor , Mohanad K. Aneed Al-Saedi , Maryam Qasim Mohammed

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引用次数: 0

摘要

背景：强直性脊柱炎（AS）是一种慢性炎症性疾病，主要影响脊柱，导致关节炎症。基因表达在AS的发病机制中起着关键作用，了解其潜在的遗传机制可以提高诊断的准确性和治疗策略。本研究旨在评估伊拉克AS患者GPR65和NUAK2基因表达水平以及骨保护素（OPG）蛋白水平。方法采用病例对照研究，选取50例AS患者和50例健康对照者。采集血样评估红细胞沉降率（ESR）， ELISA法检测血清c反应蛋白（CRP）和OPG水平。采用专用试剂盒提取总RNA，然后进行cDNA合成和定量实时PCR （qRT-PCR）分析GPR65和NUAK2的表达水平。结果OPG水平显著升高(p <；0.001**)和GPR65的表达(p <；0.001**)，同时与健康对照相比，AS患者的NUAK2表达下调（p = 0.05*）。结论OPG水平的升高和GPR65表达的增加可能参与了AS的炎症过程，而NUAK2表达的降低可能破坏了炎症的调节。此外，OPG、GPR65和NUAK2具有较强的诊断潜力，通过ROC分析显示其具有较高的特异性和敏感性。OPG和GPR65可能增强AS的诊断和监测，并强调需要进一步研究它们在疾病进展中的作用和作为潜在治疗靶点。

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Linking inflammation to gene expression: Insights from GPR65, NUAK2, and OPG in ankylosing spondylitis

Background

Ankylosing spondylitis (AS) is a chronic inflammatory disease that primarily affects the spine, leading to joint inflammation. Gene expression plays a pivotal role in the pathogenesis of AS, and understanding its underlying genetic mechanisms may enhance both diagnostic accuracy and therapeutic strategies. This study aimed to evaluate the gene expression levels of GPR65 and NUAK2, along with the protein level of Osteoprotegerin (OPG), in Iraqi individuals diagnosed with AS.

Methods

A case-control study was conducted, involving 50 patients with AS and 50 healthy controls. Blood samples were collected to assess the erythrocyte sedimentation rate (ESR), while serum levels of C-reactive protein (CRP) and OPG were measured using ELISA. Total RNA was extracted using a specialized kit, followed by cDNA synthesis and quantitative real-time PCR (qRT-PCR) to analyze the expression levels of GPR65 and NUAK2.

Results

The findings revealed a significant increase in OPG levels (p < 0.001**) and GPR65 expression (p < 0.001**), alongside a downregulation of NUAK2 expression (p = 0.05*) in AS patients compared to healthy controls.

Conclusions

These results suggest that elevated OPG levels and increased GPR65 expression may contribute to the inflammatory processes in AS, whereas reduced NUAK2 expression might impair the regulation of inflammation. Furthermore, OPG, GPR65, and NUAK2 exhibited strong diagnostic potential, with high specificity and sensitivity demonstrated through ROC analysis. The OPG and GPR65 may enhance the diagnosis and monitoring of AS and underscore the need for further research into their roles in disease progression and as potential targets for therapy.

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.