Zarin Taj, B Chithradevi, Kavya Bakka, Dinakar Challabathula
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In the current study, inoculation of Providencia rettgeri ST202, a halotolerant rhizospheric bacteria isolated from rhizospheric soil of salt pan areas, resulted in plant growth-promoting traits along with improvement in growth of Oryza sativa and Lycopersicon esculentum plants under normal and salinity stress (S2: 200 mM and S4: 400 mM NaCl) conditions prompting us to evaluate changes in physiological and biochemical parameters, photosynthesis, photorespiration, reactive oxygen species (ROS), and antioxidants. Compared to uninoculated plants, improved relative water content, chlorophyll content, carotenoids, and antioxidant levels were observed along with decreased electrolyte leakage and ROS in ST202 inoculated rice and tomato plants suggesting better protection under control and salinity stress. While salinity stress severely impacted photosynthesis under different light intensities and different CO<sub>2</sub> levels in uninoculated plants, ST202 inoculated plants have higher photosynthetic rates. Spider plot comparison of gas exchange and chlorophyll fluorescence parameters revealed superior performance of ST202 inoculated plants. The mesophyll conductance and chloroplastic CO<sub>2</sub> concentration were maintained at higher level in ST202 inoculated plants showing alterations in limitation in stomatal conductance, mesophyll conductance, and biochemical limitation suggesting beneficial modulation by PGPR for improving photosynthesis under salt stress conditions. Our results indicated modulation of key photosynthetic processes along with significant reduction in ROS by the halotolerant PGPR P. rettgeri ST202 towards enhanced salinity stress tolerance of rice and tomato plants.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"262 4","pages":"78"},"PeriodicalIF":3.8000,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Modulation in key physiological traits improved ROS scavenging and salinity tolerance amelioration in rice and tomato inoculated with halotolerant rhizobacteria Providencia rettgeri ST202.\",\"authors\":\"Zarin Taj, B Chithradevi, Kavya Bakka, Dinakar Challabathula\",\"doi\":\"10.1007/s00425-025-04800-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Main conclusion: </strong>Halotolerant rhizospheric bacteria Providencia rettgeri ST202 positively modulated the changes in stomatal, mesophyll, and biochemical limitations, fine-tuning photosynthesis, photorespiration, ROS, and antioxidants in rice and tomato plants for improved salinity tolerance. Soil salinity is detrimental for survival of majority of crops, since increased salt disrupts root water uptake, induces cellular ion toxicity ultimately leading to drastic decrease in crop yields. In the current study, inoculation of Providencia rettgeri ST202, a halotolerant rhizospheric bacteria isolated from rhizospheric soil of salt pan areas, resulted in plant growth-promoting traits along with improvement in growth of Oryza sativa and Lycopersicon esculentum plants under normal and salinity stress (S2: 200 mM and S4: 400 mM NaCl) conditions prompting us to evaluate changes in physiological and biochemical parameters, photosynthesis, photorespiration, reactive oxygen species (ROS), and antioxidants. 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引用次数: 0
摘要
主要结论:耐盐根际细菌Providencia rettgeri ST202正调节水稻和番茄植株气孔、叶肉和生化限制的变化,微调光合作用、光呼吸、ROS和抗氧化剂,从而提高水稻和番茄的耐盐性。土壤盐分对大多数作物的生存是有害的,因为盐分增加会破坏根系对水的吸收,诱发细胞离子毒性,最终导致作物产量急剧下降。本研究通过接种从盐田根际土壤中分离出来的耐盐根际细菌proprovidencia rettgeri ST202,在正常胁迫和盐胁迫(S2: 200 mM和S4: 200 mM)下,水稻和番茄的生长均有促进植物生长的特性。400 mM NaCl)条件下,我们评估了生理生化参数、光合作用、光呼吸、活性氧(ROS)和抗氧化剂的变化。与未接种ST202的植株相比,接种ST202的水稻和番茄植株的相对含水量、叶绿素含量、类胡萝卜素和抗氧化剂水平均有所提高,电解质泄漏和ROS均有所减少,表明ST202在控制和盐胁迫下具有更好的保护作用。在不同光强和不同CO2浓度下,盐胁迫严重影响未接种植株的光合作用,但ST202接种植株的光合速率更高。通过蜘蛛图比较气体交换和叶绿素荧光参数,发现ST202接种植株表现优异。ST202接种植株的叶肉导度和叶绿体CO2浓度维持在较高水平,表明在气孔导度限制、叶肉导度限制和生化限制方面发生了变化,表明PGPR对盐胁迫条件下光合作用的改善具有有益的调控作用。我们的研究结果表明,耐盐PGPR P. rettgeri ST202通过调控关键光合过程和显著降低ROS,提高了水稻和番茄植株的耐盐性。
Modulation in key physiological traits improved ROS scavenging and salinity tolerance amelioration in rice and tomato inoculated with halotolerant rhizobacteria Providencia rettgeri ST202.
Main conclusion: Halotolerant rhizospheric bacteria Providencia rettgeri ST202 positively modulated the changes in stomatal, mesophyll, and biochemical limitations, fine-tuning photosynthesis, photorespiration, ROS, and antioxidants in rice and tomato plants for improved salinity tolerance. Soil salinity is detrimental for survival of majority of crops, since increased salt disrupts root water uptake, induces cellular ion toxicity ultimately leading to drastic decrease in crop yields. In the current study, inoculation of Providencia rettgeri ST202, a halotolerant rhizospheric bacteria isolated from rhizospheric soil of salt pan areas, resulted in plant growth-promoting traits along with improvement in growth of Oryza sativa and Lycopersicon esculentum plants under normal and salinity stress (S2: 200 mM and S4: 400 mM NaCl) conditions prompting us to evaluate changes in physiological and biochemical parameters, photosynthesis, photorespiration, reactive oxygen species (ROS), and antioxidants. Compared to uninoculated plants, improved relative water content, chlorophyll content, carotenoids, and antioxidant levels were observed along with decreased electrolyte leakage and ROS in ST202 inoculated rice and tomato plants suggesting better protection under control and salinity stress. While salinity stress severely impacted photosynthesis under different light intensities and different CO2 levels in uninoculated plants, ST202 inoculated plants have higher photosynthetic rates. Spider plot comparison of gas exchange and chlorophyll fluorescence parameters revealed superior performance of ST202 inoculated plants. The mesophyll conductance and chloroplastic CO2 concentration were maintained at higher level in ST202 inoculated plants showing alterations in limitation in stomatal conductance, mesophyll conductance, and biochemical limitation suggesting beneficial modulation by PGPR for improving photosynthesis under salt stress conditions. Our results indicated modulation of key photosynthetic processes along with significant reduction in ROS by the halotolerant PGPR P. rettgeri ST202 towards enhanced salinity stress tolerance of rice and tomato plants.
期刊介绍:
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