Soad M Yehia, Yousseria M Shetaia, Ghoson M Daba, Faten A Mostafa, Ali A Ali, Hassaan El-Menoufy
{"title":"一种新型麦氏菌酸产菌awamori Aspergillus MH2：生产优化及应用。","authors":"Soad M Yehia, Yousseria M Shetaia, Ghoson M Daba, Faten A Mostafa, Ali A Ali, Hassaan El-Menoufy","doi":"10.1186/s12934-025-02804-y","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Producing bio-based chemicals using a straightforward and ecologically responsible biotechnological method is intriguing. Maltobionic acid (MBA) is an aldobionic acid obtained from maltose oxidation and is an industrially applied compound. Having antioxidative and antimicrobial, non-toxic, highly water soluble, moisturizing, metal chelating, mildly sour and slightly sweet characteristics.</p><p><strong>Results: </strong>This study succeeded in utilizing Aspergillus awamori as a novel MBA producer. MBA production was improved through two-step statistical factorial designs. Plackett-Burman Design (PBD) investigating the qualitative interaction between eleven factors (maltose, KNO<sub>3</sub>, NaCl, KH<sub>2</sub>PO<sub>4</sub>, K<sub>2</sub>HPO<sub>4</sub>, MgSO<sub>4</sub>, FeSO<sub>4</sub>.7H<sub>2</sub>O, initial pH, temperature, incubation time and rpm) on MBA production causing 1.37- fold increase. Central Composite design (CCD) analyzing the quantitative interaction between the most MBA production affecting factors (temperature, rpm, KH<sub>2</sub>PO<sub>4</sub>, and incubation period) gave a 1.64- improvement in MBA production compared with un-optimized medium. The addition of agro-industrial wastes (AIW) (corn cobs and artichoke leaves) to optimized medium g/l (maltose, 5; KNO<sub>3</sub>, 1; KH<sub>2</sub>PO<sub>4</sub>, 1.0; MgSO<sub>4</sub>, 0.5; FeSO<sub>4</sub>.7(H<sub>2</sub>O), 0.02; pH, 5.00 at 37.5 °C for 9.5 days at 125 rpm) caused 2.41- and 1.97- fold increase in MBA production, respectively in comparison with the initial production conditions. MBA produced by A. awamori MH2 exerted an anti-oxidant activity with a ratio of 86% using DPPH scavenging assay.</p>","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":"24 1","pages":"181"},"PeriodicalIF":4.9000,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12337431/pdf/","citationCount":"0","resultStr":"{\"title\":\"Aspergillus awamori MH2 as a novel maltobionic acid producer: production optimization and application.\",\"authors\":\"Soad M Yehia, Yousseria M Shetaia, Ghoson M Daba, Faten A Mostafa, Ali A Ali, Hassaan El-Menoufy\",\"doi\":\"10.1186/s12934-025-02804-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Producing bio-based chemicals using a straightforward and ecologically responsible biotechnological method is intriguing. Maltobionic acid (MBA) is an aldobionic acid obtained from maltose oxidation and is an industrially applied compound. Having antioxidative and antimicrobial, non-toxic, highly water soluble, moisturizing, metal chelating, mildly sour and slightly sweet characteristics.</p><p><strong>Results: </strong>This study succeeded in utilizing Aspergillus awamori as a novel MBA producer. MBA production was improved through two-step statistical factorial designs. Plackett-Burman Design (PBD) investigating the qualitative interaction between eleven factors (maltose, KNO<sub>3</sub>, NaCl, KH<sub>2</sub>PO<sub>4</sub>, K<sub>2</sub>HPO<sub>4</sub>, MgSO<sub>4</sub>, FeSO<sub>4</sub>.7H<sub>2</sub>O, initial pH, temperature, incubation time and rpm) on MBA production causing 1.37- fold increase. Central Composite design (CCD) analyzing the quantitative interaction between the most MBA production affecting factors (temperature, rpm, KH<sub>2</sub>PO<sub>4</sub>, and incubation period) gave a 1.64- improvement in MBA production compared with un-optimized medium. The addition of agro-industrial wastes (AIW) (corn cobs and artichoke leaves) to optimized medium g/l (maltose, 5; KNO<sub>3</sub>, 1; KH<sub>2</sub>PO<sub>4</sub>, 1.0; MgSO<sub>4</sub>, 0.5; FeSO<sub>4</sub>.7(H<sub>2</sub>O), 0.02; pH, 5.00 at 37.5 °C for 9.5 days at 125 rpm) caused 2.41- and 1.97- fold increase in MBA production, respectively in comparison with the initial production conditions. 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Aspergillus awamori MH2 as a novel maltobionic acid producer: production optimization and application.
Background: Producing bio-based chemicals using a straightforward and ecologically responsible biotechnological method is intriguing. Maltobionic acid (MBA) is an aldobionic acid obtained from maltose oxidation and is an industrially applied compound. Having antioxidative and antimicrobial, non-toxic, highly water soluble, moisturizing, metal chelating, mildly sour and slightly sweet characteristics.
Results: This study succeeded in utilizing Aspergillus awamori as a novel MBA producer. MBA production was improved through two-step statistical factorial designs. Plackett-Burman Design (PBD) investigating the qualitative interaction between eleven factors (maltose, KNO3, NaCl, KH2PO4, K2HPO4, MgSO4, FeSO4.7H2O, initial pH, temperature, incubation time and rpm) on MBA production causing 1.37- fold increase. Central Composite design (CCD) analyzing the quantitative interaction between the most MBA production affecting factors (temperature, rpm, KH2PO4, and incubation period) gave a 1.64- improvement in MBA production compared with un-optimized medium. The addition of agro-industrial wastes (AIW) (corn cobs and artichoke leaves) to optimized medium g/l (maltose, 5; KNO3, 1; KH2PO4, 1.0; MgSO4, 0.5; FeSO4.7(H2O), 0.02; pH, 5.00 at 37.5 °C for 9.5 days at 125 rpm) caused 2.41- and 1.97- fold increase in MBA production, respectively in comparison with the initial production conditions. MBA produced by A. awamori MH2 exerted an anti-oxidant activity with a ratio of 86% using DPPH scavenging assay.
期刊介绍：
Microbial Cell Factories is an open access peer-reviewed journal that covers any topic related to the development, use and investigation of microbial cells as producers of recombinant proteins and natural products, or as catalyzers of biological transformations of industrial interest. Microbial Cell Factories is the world leading, primary research journal fully focusing on Applied Microbiology.
The journal is divided into the following editorial sections:
-Metabolic engineering
-Synthetic biology
-Whole-cell biocatalysis
-Microbial regulations
-Recombinant protein production/bioprocessing
-Production of natural compounds
-Systems biology of cell factories
-Microbial production processes
-Cell-free systems
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