利用CRISPR/Cas9技术生成两个B2M敲除诱导的多能干细胞系（DHMi005-A-8和DHMi005-A-9）。

IF 0.7 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-09-01 Epub Date: 2025-08-06 DOI:10.1016/j.scr.2025.103785

Katharina Abdel-Malek, Franziska von Eisenhart-Rothe, Santiago Stiegmann, Irina Gottmann, Stefanie A Doppler, Stephanie Schneider, Heike Preisler, Harald Lahm, Martina Dreßen

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引用次数: 0

摘要

将细胞移植到受体生物体中有许多障碍需要克服，包括t细胞触发的细胞免疫防御问题。细胞排斥反应是基于mhc - i复合物的抗原呈递，它被细胞毒性T淋巴细胞识别。通过敲除B2M （β -2微球蛋白）亚基来消除mhc - i复合体可能是减少甚至完全阻止移植过程中初始细胞免疫反应的一种方法。利用CRISPR/Cas9，我们建立了一个杂合和一个纯合的B2M敲除诱导多能干细胞（iPSC）系，作为更有效的细胞移植的第一步。

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Generation of two B2M knockout induced pluripotent stem cell lines (DHMi005-A-8 and DHMi005-A-9) using CRISPR/Cas9 technology.

The transplantation of cells into a recipient organism has many hurdles to overcome, including the problem of T-cell-triggered cellular immune defense. Cellular rejection is based on antigen presentation by the MHC-I-complex, which is recognized by cytotoxic T lymphocytes. Elimination of the MHC-I-complex by knocking out the B2M (Beta-2-microglobulin) subunit may be one way of reducing or even completely preventing the initial cellular immune response during transplantation. Using CRISPR/Cas9 we established one heterozygous and one homozygous B2M knockout induced pluripotent stem cell (iPSC) line as a first step towards more effective cell transplantation.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.