桉树对侵袭性叶枯病病原菌侵染的转录反应揭示了寄主次生代谢物在病原菌萌发中的作用。

IF 3.8 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Myriam Solís, Almuth Hammerbacher, Michael J Wingfield, Sanushka Naidoo
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引用次数: 0

摘要

由破坏性畸圆虫引起的畸圆虫叶枯病严重威胁着世界各地的桉树人工林。病原菌在接种后24 ~ 72 h通过气孔渗透侵染叶片。两周后症状可见,病原体孢子通常在接种易感宿主四周后出现。研究了一个桉树易感无性系在整个疾病周期内的反应,以确定易感因子。在接种后3、14和28天分别从健康和感染叶片中分离RNA。差异表达和基因富集分析显示,参与水杨酸和脱落酸途径的转录因子家族成员TGA和MYB以及参与这些激素信号通路的基因均上调。总体而言,植物防御反应途径仅在感染后期(28 dpi)富集。相比之下,基因表达和化学分析表明,桉树叶片中主要黄酮类化合物的合成在病原菌侵染过程中增强,而萜类化合物和黄烷-3-醇的合成则下降。黄酮醇、芦丁和槲皮素促进了孢子的萌发,而萜类桉树醇和黄烷-3-醇儿茶素抑制了孢子的萌发。本研究提供了在不同阶段的敏感宿主的分子和化学反应的见解,从而提高了目前的病理系统的认识。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Transcriptional responses of Eucalyptus to infection by an aggressive leaf blight pathogen reveal the role of host secondary metabolites during pathogen germination.

Teratosphaeria leaf blight disease caused by Teratosphaeria destructans poses a serious threat to Eucalyptus plantations worldwide. The pathogen infects leaves via stomatal penetration from 24 to 72 h after inoculation. Symptoms are visible after two weeks and pathogen sporulation commonly occurs four weeks after inoculation of a susceptible host. We studied the responses of a susceptible Eucalyptus clone during the entire disease cycle to identify susceptibility factors. RNA from healthy and infected leaves was isolated at 3, 14 and 28 days post inoculation. Differential expression and gene enrichment analysis showed that members of the transcription factor family TGA and MYB, involved in the salicylic acid and abscisic acid pathways, and genes involved in these hormone signaling pathways, were up-regulated. Overall, plant defense response pathways were enriched only at the late stage of infection (28 dpi). In contrast, both gene expression and chemical analysis revealed that the synthesis of the major flavonoids in Eucalyptus leaves was enhanced during pathogen infection, while the synthesis of terpenoids and flavan-3-ols declined. The flavonols, rutin and quercetin enhanced spore germination in-vitro while, the terpenoid eucalyptol and the flavan-3-ol catechin inhibited germination. This study provides insights into the molecular and chemical responses at different stages of infection of a susceptible host by T. destructans, thereby improving the current understanding of the pathosystem.

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来源期刊
Plant Molecular Biology
Plant Molecular Biology 生物-生化与分子生物学
自引率
2.00%
发文量
95
审稿时长
1.4 months
期刊介绍: Plant Molecular Biology is an international journal dedicated to rapid publication of original research articles in all areas of plant biology.The Editorial Board welcomes full-length manuscripts that address important biological problems of broad interest, including research in comparative genomics, functional genomics, proteomics, bioinformatics, computational biology, biochemical and regulatory networks, and biotechnology. Because space in the journal is limited, however, preference is given to publication of results that provide significant new insights into biological problems and that advance the understanding of structure, function, mechanisms, or regulation. Authors must ensure that results are of high quality and that manuscripts are written for a broad plant science audience.
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