{"title":"生血消斑胶囊对ITP小鼠模型Tregs的影响。","authors":"Jianqiang Liu, Xiaoyan Li, Shenggui Hou, Aixia Dou","doi":"10.1097/MS9.0000000000003572","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>To study the effect of Sheng Xuexiaoban capsules (SXXCs) on restoring immune dysfunction in ITP model mice.</p><p><strong>Methods: </strong>We assessed changes in platelet counts, megakaryocyte counts, Treg functions, interleukin-2 expression, and other indicators to investigate the effect of SXXCs in the ITP mouse model.</p><p><strong>Results: </strong>After treatment of the ITP mouse model with SXXCs, we found increases in platelets (577.35 ± 76.94 vs. 123.33 ± 16.24, <i>P</i> < 0.05) and the Treg number (7.35 ± 1.94 vs. 1.33 ± 1.24), and Foxp3 mRNA expression (2<sup>-ΔCt</sup>: 0.2493 ± 0.03102 vs. 0.683 ± 0.05774, <i>P</i> = 0.0027). Decreased interleukin-2 produced by effector T cells was detected (2<sup>-ΔCt</sup>: 0.6119 ± 0.01173 vs. 2.838 ± 0.1004, <i>P</i> < 0.001). SXXCs treatment significantly improved the proportion and function of Tregs. By increasing Foxp3 mRNA expression and decreasing interleukin-2, we restored immune tolerance to achieve a good therapeutic effect in ITP model mice.</p><p><strong>Conclusions: </strong>We confirmed that SXXCs have a good therapeutic effect on ITP. Simultaneously we verified the important role of Tregs in ITP pathogenesis.</p>","PeriodicalId":8025,"journal":{"name":"Annals of Medicine and Surgery","volume":"87 8","pages":"4848-4852"},"PeriodicalIF":1.6000,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12333745/pdf/","citationCount":"0","resultStr":"{\"title\":\"Effect of Sheng Xuexiaoban capsules on Tregs in an ITP mouse model.\",\"authors\":\"Jianqiang Liu, Xiaoyan Li, Shenggui Hou, Aixia Dou\",\"doi\":\"10.1097/MS9.0000000000003572\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objectives: </strong>To study the effect of Sheng Xuexiaoban capsules (SXXCs) on restoring immune dysfunction in ITP model mice.</p><p><strong>Methods: </strong>We assessed changes in platelet counts, megakaryocyte counts, Treg functions, interleukin-2 expression, and other indicators to investigate the effect of SXXCs in the ITP mouse model.</p><p><strong>Results: </strong>After treatment of the ITP mouse model with SXXCs, we found increases in platelets (577.35 ± 76.94 vs. 123.33 ± 16.24, <i>P</i> < 0.05) and the Treg number (7.35 ± 1.94 vs. 1.33 ± 1.24), and Foxp3 mRNA expression (2<sup>-ΔCt</sup>: 0.2493 ± 0.03102 vs. 0.683 ± 0.05774, <i>P</i> = 0.0027). Decreased interleukin-2 produced by effector T cells was detected (2<sup>-ΔCt</sup>: 0.6119 ± 0.01173 vs. 2.838 ± 0.1004, <i>P</i> < 0.001). SXXCs treatment significantly improved the proportion and function of Tregs. By increasing Foxp3 mRNA expression and decreasing interleukin-2, we restored immune tolerance to achieve a good therapeutic effect in ITP model mice.</p><p><strong>Conclusions: </strong>We confirmed that SXXCs have a good therapeutic effect on ITP. Simultaneously we verified the important role of Tregs in ITP pathogenesis.</p>\",\"PeriodicalId\":8025,\"journal\":{\"name\":\"Annals of Medicine and Surgery\",\"volume\":\"87 8\",\"pages\":\"4848-4852\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2025-07-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12333745/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Annals of Medicine and Surgery\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1097/MS9.0000000000003572\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/8/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"MEDICINE, GENERAL & INTERNAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annals of Medicine and Surgery","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1097/MS9.0000000000003572","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/8/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"MEDICINE, GENERAL & INTERNAL","Score":null,"Total":0}
引用次数: 0
摘要
目的：研究生血消斑胶囊（SXXCs）对ITP模型小鼠免疫功能障碍的恢复作用。方法：通过观察血小板计数、巨核细胞计数、Treg功能、白细胞介素-2表达等指标的变化，探讨SXXCs对ITP小鼠模型的影响。结果：SXXCs治疗ITP小鼠模型后，血小板（577.35±76.94 vs. 123.33±16.24,P < 0.05）、Treg数（7.35±1.94 vs. 1.33±1.24）、Foxp3 mRNA表达（2-ΔCt: 0.2493±0.03102 vs. 0.683±0.05774,P = 0.0027）增加。效应T细胞产生的白细胞介素-2减少（2-ΔCt: 0.6119±0.01173 vs. 2.838±0.1004,P < 0.001）。SXXCs处理显著提高了Tregs的比例和功能。通过提高Foxp3 mRNA表达，降低白细胞介素-2，恢复ITP模型小鼠的免疫耐受，达到良好的治疗效果。结论：我们证实SXXCs对ITP有良好的治疗效果。同时证实Tregs在ITP发病机制中的重要作用。
Effect of Sheng Xuexiaoban capsules on Tregs in an ITP mouse model.
Objectives: To study the effect of Sheng Xuexiaoban capsules (SXXCs) on restoring immune dysfunction in ITP model mice.
Methods: We assessed changes in platelet counts, megakaryocyte counts, Treg functions, interleukin-2 expression, and other indicators to investigate the effect of SXXCs in the ITP mouse model.
Results: After treatment of the ITP mouse model with SXXCs, we found increases in platelets (577.35 ± 76.94 vs. 123.33 ± 16.24, P < 0.05) and the Treg number (7.35 ± 1.94 vs. 1.33 ± 1.24), and Foxp3 mRNA expression (2-ΔCt: 0.2493 ± 0.03102 vs. 0.683 ± 0.05774, P = 0.0027). Decreased interleukin-2 produced by effector T cells was detected (2-ΔCt: 0.6119 ± 0.01173 vs. 2.838 ± 0.1004, P < 0.001). SXXCs treatment significantly improved the proportion and function of Tregs. By increasing Foxp3 mRNA expression and decreasing interleukin-2, we restored immune tolerance to achieve a good therapeutic effect in ITP model mice.
Conclusions: We confirmed that SXXCs have a good therapeutic effect on ITP. Simultaneously we verified the important role of Tregs in ITP pathogenesis.
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