Guanjun Ye, Jiangfei Li, Jiayu Wang, Ting Ju, Zhilong Zhou, Jianlong Wang, Haitian Zhao, Yingyu Zhou, Weihong Lu
{"title":"超声辅助提取与热水提取芦花废茎多糖的优势:效率、结构和生物活性的综合比较。","authors":"Guanjun Ye, Jiangfei Li, Jiayu Wang, Ting Ju, Zhilong Zhou, Jianlong Wang, Haitian Zhao, Yingyu Zhou, Weihong Lu","doi":"10.1016/j.ultsonch.2025.107502","DOIUrl":null,"url":null,"abstract":"<p><p>Rubia cordifolia L. (RCL) is a plant with both commercial and medicinal values, widely distributed worldwide. Its roots have been used to treat various diseases, including abnormal uterine bleeding, arthritis, and kidney stones. However, its stems are often overlooked and regarded as waste. This study employed response surface methodology to optimize the ultrasonic-assisted extraction (UAE) process for polysaccharides from RCL waste stems (RCLP), and compared energy consumption and carbon emissions with hot water extraction (HWE). The underlying mechanism of UAE for polysaccharide extraction was further explored. Additionally, the physicochemical properties and in vitro activities of RCLP extracted by UAE (UAE-P) and HWE (HWE-P) were evaluated and compared. The results showed that the optimal UAE conditions were as follows: temperature of 52.5 °C, extraction time of 43 min, power of 310 W, and liquid-to-solid ratio of 32 mL/g, yielding 5.97 ± 0.34 % of RCLP. Compared with HWE, UAE significantly improved extraction efficiency, with a 640.18 % increase in production rate and a 93.23 % reduction in specific energy. UAE promoted the exposure of cell wall functional groups (e.g., C=O, C-O-C) and disintegration of the crystalline structure, thereby enhancing extraction efficiency. UAE-P exhibited higher uronic acid content, smaller and more uniform particle size, and better stability than HWE-P. These structural characteristics were significantly correlated with superior in vitro antioxidant and hypoglycemic activities, as well as stronger bile salt binding capacity. In conclusion, this study highlights that UAE is a feasible method for efficiently extracting high-value polysaccharides from RCL waste stems.</p>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"120 ","pages":"107502"},"PeriodicalIF":9.7000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12357284/pdf/","citationCount":"0","resultStr":"{\"title\":\"Advantages of ultrasonic-assisted extraction over hot water extraction for polysaccharides from waste stems of Rubia cordifolia L.: A comprehensive comparison of efficiency, structure, and bioactivity.\",\"authors\":\"Guanjun Ye, Jiangfei Li, Jiayu Wang, Ting Ju, Zhilong Zhou, Jianlong Wang, Haitian Zhao, Yingyu Zhou, Weihong Lu\",\"doi\":\"10.1016/j.ultsonch.2025.107502\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Rubia cordifolia L. (RCL) is a plant with both commercial and medicinal values, widely distributed worldwide. Its roots have been used to treat various diseases, including abnormal uterine bleeding, arthritis, and kidney stones. However, its stems are often overlooked and regarded as waste. This study employed response surface methodology to optimize the ultrasonic-assisted extraction (UAE) process for polysaccharides from RCL waste stems (RCLP), and compared energy consumption and carbon emissions with hot water extraction (HWE). The underlying mechanism of UAE for polysaccharide extraction was further explored. Additionally, the physicochemical properties and in vitro activities of RCLP extracted by UAE (UAE-P) and HWE (HWE-P) were evaluated and compared. The results showed that the optimal UAE conditions were as follows: temperature of 52.5 °C, extraction time of 43 min, power of 310 W, and liquid-to-solid ratio of 32 mL/g, yielding 5.97 ± 0.34 % of RCLP. Compared with HWE, UAE significantly improved extraction efficiency, with a 640.18 % increase in production rate and a 93.23 % reduction in specific energy. UAE promoted the exposure of cell wall functional groups (e.g., C=O, C-O-C) and disintegration of the crystalline structure, thereby enhancing extraction efficiency. UAE-P exhibited higher uronic acid content, smaller and more uniform particle size, and better stability than HWE-P. These structural characteristics were significantly correlated with superior in vitro antioxidant and hypoglycemic activities, as well as stronger bile salt binding capacity. In conclusion, this study highlights that UAE is a feasible method for efficiently extracting high-value polysaccharides from RCL waste stems.</p>\",\"PeriodicalId\":442,\"journal\":{\"name\":\"Ultrasonics Sonochemistry\",\"volume\":\"120 \",\"pages\":\"107502\"},\"PeriodicalIF\":9.7000,\"publicationDate\":\"2025-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12357284/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Ultrasonics Sonochemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1016/j.ultsonch.2025.107502\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/8/6 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"ACOUSTICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ultrasonics Sonochemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.ultsonch.2025.107502","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/8/6 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"ACOUSTICS","Score":null,"Total":0}
Advantages of ultrasonic-assisted extraction over hot water extraction for polysaccharides from waste stems of Rubia cordifolia L.: A comprehensive comparison of efficiency, structure, and bioactivity.
Rubia cordifolia L. (RCL) is a plant with both commercial and medicinal values, widely distributed worldwide. Its roots have been used to treat various diseases, including abnormal uterine bleeding, arthritis, and kidney stones. However, its stems are often overlooked and regarded as waste. This study employed response surface methodology to optimize the ultrasonic-assisted extraction (UAE) process for polysaccharides from RCL waste stems (RCLP), and compared energy consumption and carbon emissions with hot water extraction (HWE). The underlying mechanism of UAE for polysaccharide extraction was further explored. Additionally, the physicochemical properties and in vitro activities of RCLP extracted by UAE (UAE-P) and HWE (HWE-P) were evaluated and compared. The results showed that the optimal UAE conditions were as follows: temperature of 52.5 °C, extraction time of 43 min, power of 310 W, and liquid-to-solid ratio of 32 mL/g, yielding 5.97 ± 0.34 % of RCLP. Compared with HWE, UAE significantly improved extraction efficiency, with a 640.18 % increase in production rate and a 93.23 % reduction in specific energy. UAE promoted the exposure of cell wall functional groups (e.g., C=O, C-O-C) and disintegration of the crystalline structure, thereby enhancing extraction efficiency. UAE-P exhibited higher uronic acid content, smaller and more uniform particle size, and better stability than HWE-P. These structural characteristics were significantly correlated with superior in vitro antioxidant and hypoglycemic activities, as well as stronger bile salt binding capacity. In conclusion, this study highlights that UAE is a feasible method for efficiently extracting high-value polysaccharides from RCL waste stems.
期刊介绍:
Ultrasonics Sonochemistry stands as a premier international journal dedicated to the publication of high-quality research articles primarily focusing on chemical reactions and reactors induced by ultrasonic waves, known as sonochemistry. Beyond chemical reactions, the journal also welcomes contributions related to cavitation-induced events and processing, including sonoluminescence, and the transformation of materials on chemical, physical, and biological levels.
Since its inception in 1994, Ultrasonics Sonochemistry has consistently maintained a top ranking in the "Acoustics" category, reflecting its esteemed reputation in the field. The journal publishes exceptional papers covering various areas of ultrasonics and sonochemistry. Its contributions are highly regarded by both academia and industry stakeholders, demonstrating its relevance and impact in advancing research and innovation.