利用纳米desi质谱成像揭示虹鳟鱼鱼苗中空间分辨的6PPDQ代谢

IF 8.8 2区 环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL
Nicholas Woytowich, Catherine Roberts, Phillip Ankley, Markus Brinkmann, Erik T. Krogh and Kyle D. Duncan*, 
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引用次数: 0

摘要

N-(1,3-二甲基丁基)-N ' -苯基-对苯二胺醌(6PPDQ)是一种新兴的轮胎衍生污染物,对选择的鲑鱼构成严重的环境风险。然而,6PPDQ毒性的机制目前尚未解决。虽然已经探索了6PPDQ暴露对物种特异性代谢组学的影响,但据我们所知,还没有研究调查了6PPDQ和6PPDQ生物转化产物在鲑鱼中的原位空间分布。本研究采用纳米喷雾解吸电喷雾电离(纳米desi)质谱成像(MSI)技术,对暴露于0.61 μg/L 6PPDQ浓度96 h的6周后虹鳟鱼鱼尾进行了6PPDQ代谢的可视化研究。这些数据首次提供了鱼类6PPDQ代谢的空间图谱。6PPDQ未在任何鱼类组织中检测到;然而,主要的6PPDQ生物转化产物,羟基化6PPDQ和二羟基化6PPDQ,被发现存在于暴露的鱼苗的肝脏和肠道中。此外,对非靶向纳米desi空间代谢组学的研究显示,暴露的鱼虾中枢神经系统中存在游离脂肪酸的积累。总体而言,本研究揭示了暴露于6PPDQ的鱼类的空间分解代谢,并探索了暴露于6PPDQ后内源性代谢物的不同定位。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Uncovering Spatially Resolved 6PPDQ Metabolism in Rainbow Trout Fry with Nano-DESI Mass Spectrometry Imaging

Uncovering Spatially Resolved 6PPDQ Metabolism in Rainbow Trout Fry with Nano-DESI Mass Spectrometry Imaging

N-(1,3-dimethylbutyl)-N-phenyl-p-phenylenediamine quinone (6PPDQ) is an emerging tire-derived contaminant that poses a critical environmental risk to select salmonids. However, the mechanisms of 6PPDQ toxicity are currently unresolved. While species-specific metabolomic effects of 6PPDQ exposure have been explored, to our knowledge no studies have investigated the in situ spatial distribution of 6PPDQ and 6PPDQ biotransformation products in salmonids. Herein, we employ nanospray desorption electrospray ionization (nano-DESI) mass spectrometry imaging (MSI) to visualize 6PPDQ metabolism in 6-week posthatch rainbow trout fry exposed to 0.61 μg/L of 6PPDQ for 96 h. These data provide the first spatial mapping of 6PPDQ metabolism in fishes. 6PPDQ was not observed in any fish tissues; however, primary 6PPDQ biotransformation products, hydroxylated 6PPDQ and dihydroxylated 6PPDQ, were found to localize in the liver and gut of exposed fry. Additionally, interrogation of nontargeted nano-DESI spatial metabolomics revealed an accumulation of free fatty acids in the central nervous system of exposed fry. Overall, this study uncovers the spatially resolved 6PPDQ metabolism in exposed fishes and explores the disparate localization of endogenous metabolites disrupted by 6PPDQ exposure.

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来源期刊
Environmental Science & Technology Letters Environ.
Environmental Science & Technology Letters Environ. ENGINEERING, ENVIRONMENTALENVIRONMENTAL SC-ENVIRONMENTAL SCIENCES
CiteScore
17.90
自引率
3.70%
发文量
163
期刊介绍: Environmental Science & Technology Letters serves as an international forum for brief communications on experimental or theoretical results of exceptional timeliness in all aspects of environmental science, both pure and applied. Published as soon as accepted, these communications are summarized in monthly issues. Additionally, the journal features short reviews on emerging topics in environmental science and technology.
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