重组Sj14-3-3蛋白作为血吸虫病免疫诊断抗原的初步评价

IF 1.6 4区医学 Q3 PARASITOLOGY

Experimental parasitology Pub Date : 2025-08-09 DOI:10.1016/j.exppara.2025.109000

Kevin Austin L. Ona , Jose Ma M. Angeles , Elena A. Villacorte , Katrina Theresa M. Balboa , Atcharaphan Wanlop , Adrian Miki C. Macalanda , Pilarita T. Rivera , Shin-ichiro Kawazu

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引用次数: 0

摘要

探索血吸虫病诊断的分子靶点一直是日本血吸虫研究的重点。本研究利用血吸虫感染者血清对重组Sj14-3-3蛋白（rSj14-3-3）的抗原性进行了评价。表达rSj14-3-3并进行SDS-PAGE和Western blot分析。用ELISA法检测了其抗原性。结果表明，rSj14-3-3在感染小鼠血清中具有显著的抗原性(P <；0.05)和人血清(P <；0.05)。这些结果提示rSj14-3-3可作为血吸虫病的替代诊断抗原。然而，需要进一步的研究来充分评估其免疫诊断潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Preliminary evaluation of the recombinant Sj14-3-3 protein as an immunodiagnostic antigen for schistosomiasis

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Preliminary evaluation of the recombinant Sj14-3-3 protein as an immunodiagnostic antigen for schistosomiasis

Exploring molecular targets for the diagnosis of schistosomiasis has been a key priority in Schistosoma japonicum research. In this study, the antigenicity of recombinant Sj14-3-3 protein (rSj14-3-3) was evaluated using sera from schistosome-infected subjects. rSj14-3-3 was expressed and subsequently analyzed via SDS-PAGE and Western blot. Its antigenicity was then tested with sera from schistosome-infected mice and humans through ELISA. Results showed that rSj14-3-3 had significant antigenicity when tested with infected mice sera (P < 0.05) and human sera (P < 0.05) compared to negative controls. These findings suggest that rSj14-3-3 could serve as an alternative diagnostic antigen for schistosomiasis. Nevertheless, further research is needed to fully evaluate its immunodiagnostic potential.

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来源期刊

Experimental parasitology 医学-寄生虫学

CiteScore

3.10

自引率

4.80%

发文量

160

审稿时长

3 months

期刊介绍： Experimental Parasitology emphasizes modern approaches to parasitology, including molecular biology and immunology. The journal features original research papers on the physiological, metabolic, immunologic, biochemical, nutritional, and chemotherapeutic aspects of parasites and host-parasite relationships.