链脲佐菌素(STZ)诱导的糖尿病wistar大鼠眼部抗氧化剂生物标志物的研究

Nduka Richard Ossai, Anthony Emeka Ojieh, Success Chidinma Obika
{"title":"链脲佐菌素(STZ)诱导的糖尿病wistar大鼠眼部抗氧化剂生物标志物的研究","authors":"Nduka Richard Ossai,&nbsp;Anthony Emeka Ojieh,&nbsp;Success Chidinma Obika","doi":"10.1016/j.prenap.2025.100299","DOIUrl":null,"url":null,"abstract":"<div><div>Carotenoids are essential for retinal health, preventing blue light damage, and preserving vision. Individuals with diabetes, particularly those with poor blood sugar management, are more likely to develop diabetic retinopathy and macular edema, which may reduce the efficacy of these carotenoids in avoiding eye harm. This study examined the levels of different carotenoid enzymes in diabetic Wistar rats treated with <em>streptozotocin (STZ)</em> and <em>Abelmoschus esculentus</em> ethanol extract. Forty (40) adult male Wistar rats weighing 105–215 g were randomly assigned to diabetic and non-diabetic units in four groups of five each. Diabetes was developed in Wistar rats in unit two following a single intraperitoneal dosage of STZ (60 mg/kg). Group one in each unit received no therapy, while group two received <em>A. esculentus</em> (300 mg/kg bodyweight). Group three received N-Acetyl Cysteine (NAC) (300 mg/kg body weight), and Group four received <em>A. esculentus</em> plus NAC (300 mg/kg). The drug was administered for 21 days before the rats were slaughtered and blood samples were collected for biochemical analysis. The data was analyzed using GraphPad Prism version 8.0, using mean Standard Error of Mean (SEM). Mean differences across groups were evaluated using two-way ANOVA, followed by the Turkey post-hoc test, with p-values &lt; 0.05 indicating statistical significance. The current investigation demonstrates that <em>A. esculentus</em>, both alone and in combination with NAC, has strong hypoglycemic properties and could be used to cure diabetic retinopathy.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"8 ","pages":"Article 100299"},"PeriodicalIF":0.0000,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Assessment of ocular antioxidants biomarkers in streptozotocin (STZ) induced diabetic wistar rats treated with Abelmoschus esculentus ethanol extract\",\"authors\":\"Nduka Richard Ossai,&nbsp;Anthony Emeka Ojieh,&nbsp;Success Chidinma Obika\",\"doi\":\"10.1016/j.prenap.2025.100299\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Carotenoids are essential for retinal health, preventing blue light damage, and preserving vision. Individuals with diabetes, particularly those with poor blood sugar management, are more likely to develop diabetic retinopathy and macular edema, which may reduce the efficacy of these carotenoids in avoiding eye harm. This study examined the levels of different carotenoid enzymes in diabetic Wistar rats treated with <em>streptozotocin (STZ)</em> and <em>Abelmoschus esculentus</em> ethanol extract. Forty (40) adult male Wistar rats weighing 105–215 g were randomly assigned to diabetic and non-diabetic units in four groups of five each. Diabetes was developed in Wistar rats in unit two following a single intraperitoneal dosage of STZ (60 mg/kg). Group one in each unit received no therapy, while group two received <em>A. esculentus</em> (300 mg/kg bodyweight). Group three received N-Acetyl Cysteine (NAC) (300 mg/kg body weight), and Group four received <em>A. esculentus</em> plus NAC (300 mg/kg). The drug was administered for 21 days before the rats were slaughtered and blood samples were collected for biochemical analysis. The data was analyzed using GraphPad Prism version 8.0, using mean Standard Error of Mean (SEM). Mean differences across groups were evaluated using two-way ANOVA, followed by the Turkey post-hoc test, with p-values &lt; 0.05 indicating statistical significance. The current investigation demonstrates that <em>A. esculentus</em>, both alone and in combination with NAC, has strong hypoglycemic properties and could be used to cure diabetic retinopathy.</div></div>\",\"PeriodicalId\":101014,\"journal\":{\"name\":\"Pharmacological Research - Natural Products\",\"volume\":\"8 \",\"pages\":\"Article 100299\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-07-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pharmacological Research - Natural Products\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2950199725001594\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmacological Research - Natural Products","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2950199725001594","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

类胡萝卜素对视网膜健康、防止蓝光损害和保护视力至关重要。糖尿病患者,特别是那些血糖管理不佳的人,更容易患糖尿病性视网膜病变和黄斑水肿,这可能会降低这些类胡萝卜素避免眼睛伤害的功效。本研究检测了链脲佐菌素(STZ)和沙蚕乙醇提取物对糖尿病Wistar大鼠的不同类胡萝卜素酶水平。40只体重105-215 g的成年雄性Wistar大鼠被随机分为糖尿病组和非糖尿病组,每组5只。第二组Wistar大鼠在单次腹腔注射STZ(60 mg/kg)后发生糖尿病。各组1组不给药,2组给药(300 mg/kg体重)。3组给予n -乙酰半胱氨酸(NAC)(300 mg/kg体重),4组给予牛蒡加NAC(300 mg/kg体重)。给药21天后,屠宰大鼠,采集血样进行生化分析。数据分析采用GraphPad Prism version 8.0,采用均数标准误差(Standard Error of mean, SEM)。采用双因素方差分析评估各组间的平均差异,然后进行土耳其事后检验,p值<; 0.05表示有统计学意义。目前的研究表明,无论是单独使用还是与NAC联合使用,黄芪都具有较强的降糖作用,可用于治疗糖尿病视网膜病变。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Assessment of ocular antioxidants biomarkers in streptozotocin (STZ) induced diabetic wistar rats treated with Abelmoschus esculentus ethanol extract
Carotenoids are essential for retinal health, preventing blue light damage, and preserving vision. Individuals with diabetes, particularly those with poor blood sugar management, are more likely to develop diabetic retinopathy and macular edema, which may reduce the efficacy of these carotenoids in avoiding eye harm. This study examined the levels of different carotenoid enzymes in diabetic Wistar rats treated with streptozotocin (STZ) and Abelmoschus esculentus ethanol extract. Forty (40) adult male Wistar rats weighing 105–215 g were randomly assigned to diabetic and non-diabetic units in four groups of five each. Diabetes was developed in Wistar rats in unit two following a single intraperitoneal dosage of STZ (60 mg/kg). Group one in each unit received no therapy, while group two received A. esculentus (300 mg/kg bodyweight). Group three received N-Acetyl Cysteine (NAC) (300 mg/kg body weight), and Group four received A. esculentus plus NAC (300 mg/kg). The drug was administered for 21 days before the rats were slaughtered and blood samples were collected for biochemical analysis. The data was analyzed using GraphPad Prism version 8.0, using mean Standard Error of Mean (SEM). Mean differences across groups were evaluated using two-way ANOVA, followed by the Turkey post-hoc test, with p-values < 0.05 indicating statistical significance. The current investigation demonstrates that A. esculentus, both alone and in combination with NAC, has strong hypoglycemic properties and could be used to cure diabetic retinopathy.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信