豇豆花叶病毒抗癌候选药物的储存稳定性-两年更新。

IF 3.9

Nanomedicine (London, England) Pub Date : 2025-10-01 Epub Date: 2025-08-09 DOI:10.1080/17435889.2025.2544522

Andrea Simms, Narek Minasov, Nicole F Steinmetz

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引用次数: 0

摘要

目的：评价豇豆花叶病毒（CPMV）贮藏2年后的结构稳定性和生物活性。材料和方法：CPMV于室温、4℃、-20℃保存2年。采用动态光散射（DLS）、粒径隔离色谱（SEC）、天然凝胶电泳和变性凝胶电泳以及透射电子显微镜（TEM）检测结构稳定性。生物活性通过B16F10真皮小鼠黑色素瘤模型和豇豆植物感染刺激的抗肿瘤功效研究来评估。结果：CPMV在-20°C条件下保存完好。在4°C下储存会引起聚集、s蛋白裂解和RNA降解——样品在4°C下储存的抗肿瘤效果降低，但保留了植物感染性。CPMV储存于RT后降解，失去生物活性。结论：冷冻保存可使CPMV保持至少2年的结构和生物稳定性。

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Storage stability of cowpea mosaic virus cancer drug candidate - a two-year update.

Aim: To evaluate the structural stability and biological activity of cowpea mosaic virus (CPMV) after 2 years of storage.

Materials and methods: CPMV was stored at room temperature (RT), 4°C, and -20°C for 2 years. Structural stability was assayed using dynamic light scattering (DLS), size exclusion chromatography (SEC), native and denaturing gel electrophoresis, and transmission electron microscopy (TEM). Biological activity was assessed by anti-tumor efficacy studies using a B16F10 dermal mouse melanoma model and by a cowpea plant infection challenge.

Results: Storage at -20°C preserved CPMV's structural properties and biological activity. Storage at 4°C caused aggregation, S-protein cleavage, and RNA degradation - samples stored at 4°C had decreased anti-tumor efficacy yet retained plant infectivity. CPMV stored at RT was degraded and lost biological activity.

Conclusion: Frozen storage allows CPMV to maintain structural and biological stability for at least 2 years.

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Nanomedicine (London, England)

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