{"title":"2021年和2022年大流行中SARS- CoV-2感染后儿童肠道微生物群的变化","authors":"Geng-Hao Bai, Hung-Yen Cheng, Sheng-Chieh Lin, Yi-Hsiang Hsu, Yin-Tai Tsai, Yu-Chen Yang, Yuan-Hung Wang, Shih-Yen Chen","doi":"10.1016/j.pedneo.2025.06.005","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused significant mortality and morbidity worldwide. Although the role of intestinal microbiota in adults with SARS-CoV-2 infection has been studied, limited research exists on children. This study was performed to investigate the clinical significance and association of intestinal microbiota composition in children with SARS-CoV-2 infection during the 2021 and 2022 pandemic waves in Taiwan.</p><p><strong>Methods: </strong>Pediatric patients diagnosed with SARS-CoV-2 via real-time reverse transcription polymerase chain reaction were randomly enrolled from Shang-Ho Hospital. The study period covered the two SARS-CoV-2 outbreaks in Taiwan in 2021 and 2022. Medical records were reviewed for clinical manifestations and cycle threshold (Ct) values, and fecal microbiota enrichment analysis was performed using next-generation sequencing and the linear discriminant analysis effect size method.</p><p><strong>Results: </strong>In total, 21 pediatric patients with confirmed SARS-CoV-2 infection were enrolled-8 from the 2021 pandemic and 13 from the 2022 pandemic. Compared with the 2021 cohort, children infected in 2022 exhibited significantly higher rates of hospitalization (P < 0.001), high fever (>39 °C) (P = 0.0002), and dehydration (P = 0.0001). By contrast, a higher prevalence of a family history of COVID-19 was observed in the 2021 group (P = 0.018). Permutational multivariate analysis of group dispersions (β-dispersion analysis) revealed a trend toward increased β-diversity in the 2021 group (P = 0.06394), along with a higher relative abundance of the genera Peptoniphilus, Fusobacterium, and Morganella.</p><p><strong>Conclusion: </strong>Our study highlights differences in clinical presentations between the 2021 and 2022 SARS-CoV-2 pandemic waves and suggests an impact of infection on the gut microbiota in children, with marginally higher β-diversity observed in 2021. These findings provide a foundation for future research into the role of intestinal microbiota in pediatric SARS-CoV-2 infection.</p>","PeriodicalId":56095,"journal":{"name":"Pediatrics and Neonatology","volume":" ","pages":""},"PeriodicalIF":2.1000,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Alteration of the gut microbiota in children following SARS- CoV-2 infection in 2021 and 2022 pandemics.\",\"authors\":\"Geng-Hao Bai, Hung-Yen Cheng, Sheng-Chieh Lin, Yi-Hsiang Hsu, Yin-Tai Tsai, Yu-Chen Yang, Yuan-Hung Wang, Shih-Yen Chen\",\"doi\":\"10.1016/j.pedneo.2025.06.005\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused significant mortality and morbidity worldwide. Although the role of intestinal microbiota in adults with SARS-CoV-2 infection has been studied, limited research exists on children. This study was performed to investigate the clinical significance and association of intestinal microbiota composition in children with SARS-CoV-2 infection during the 2021 and 2022 pandemic waves in Taiwan.</p><p><strong>Methods: </strong>Pediatric patients diagnosed with SARS-CoV-2 via real-time reverse transcription polymerase chain reaction were randomly enrolled from Shang-Ho Hospital. The study period covered the two SARS-CoV-2 outbreaks in Taiwan in 2021 and 2022. Medical records were reviewed for clinical manifestations and cycle threshold (Ct) values, and fecal microbiota enrichment analysis was performed using next-generation sequencing and the linear discriminant analysis effect size method.</p><p><strong>Results: </strong>In total, 21 pediatric patients with confirmed SARS-CoV-2 infection were enrolled-8 from the 2021 pandemic and 13 from the 2022 pandemic. Compared with the 2021 cohort, children infected in 2022 exhibited significantly higher rates of hospitalization (P < 0.001), high fever (>39 °C) (P = 0.0002), and dehydration (P = 0.0001). By contrast, a higher prevalence of a family history of COVID-19 was observed in the 2021 group (P = 0.018). Permutational multivariate analysis of group dispersions (β-dispersion analysis) revealed a trend toward increased β-diversity in the 2021 group (P = 0.06394), along with a higher relative abundance of the genera Peptoniphilus, Fusobacterium, and Morganella.</p><p><strong>Conclusion: </strong>Our study highlights differences in clinical presentations between the 2021 and 2022 SARS-CoV-2 pandemic waves and suggests an impact of infection on the gut microbiota in children, with marginally higher β-diversity observed in 2021. These findings provide a foundation for future research into the role of intestinal microbiota in pediatric SARS-CoV-2 infection.</p>\",\"PeriodicalId\":56095,\"journal\":{\"name\":\"Pediatrics and Neonatology\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2025-07-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pediatrics and Neonatology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1016/j.pedneo.2025.06.005\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PEDIATRICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pediatrics and Neonatology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.pedneo.2025.06.005","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PEDIATRICS","Score":null,"Total":0}
Alteration of the gut microbiota in children following SARS- CoV-2 infection in 2021 and 2022 pandemics.
Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused significant mortality and morbidity worldwide. Although the role of intestinal microbiota in adults with SARS-CoV-2 infection has been studied, limited research exists on children. This study was performed to investigate the clinical significance and association of intestinal microbiota composition in children with SARS-CoV-2 infection during the 2021 and 2022 pandemic waves in Taiwan.
Methods: Pediatric patients diagnosed with SARS-CoV-2 via real-time reverse transcription polymerase chain reaction were randomly enrolled from Shang-Ho Hospital. The study period covered the two SARS-CoV-2 outbreaks in Taiwan in 2021 and 2022. Medical records were reviewed for clinical manifestations and cycle threshold (Ct) values, and fecal microbiota enrichment analysis was performed using next-generation sequencing and the linear discriminant analysis effect size method.
Results: In total, 21 pediatric patients with confirmed SARS-CoV-2 infection were enrolled-8 from the 2021 pandemic and 13 from the 2022 pandemic. Compared with the 2021 cohort, children infected in 2022 exhibited significantly higher rates of hospitalization (P < 0.001), high fever (>39 °C) (P = 0.0002), and dehydration (P = 0.0001). By contrast, a higher prevalence of a family history of COVID-19 was observed in the 2021 group (P = 0.018). Permutational multivariate analysis of group dispersions (β-dispersion analysis) revealed a trend toward increased β-diversity in the 2021 group (P = 0.06394), along with a higher relative abundance of the genera Peptoniphilus, Fusobacterium, and Morganella.
Conclusion: Our study highlights differences in clinical presentations between the 2021 and 2022 SARS-CoV-2 pandemic waves and suggests an impact of infection on the gut microbiota in children, with marginally higher β-diversity observed in 2021. These findings provide a foundation for future research into the role of intestinal microbiota in pediatric SARS-CoV-2 infection.
期刊介绍：
Pediatrics and Neonatology is the official peer-reviewed publication of the Taiwan Pediatric Association and The Society of Neonatology ROC, and is indexed in EMBASE and SCOPUS. Articles on clinical and laboratory research in pediatrics and related fields are eligible for consideration.
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