韩国貉粪便抗性组和致病基因组的空间季节性比较。

IF 2.2 3区 环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES
Priyanka Kumari, Binu M Tripathi, Kyung Yeon Eo, Junpei Kimura, Naomichi Yamamoto
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引用次数: 0

摘要

浣熊(学名:Nyctereutes procyonoides)是一种中型杂食性动物,原产于亚洲。由于它们生活在人类居住地附近,因此,浣熊狗与人类之间可能存在双向影响,因此调查它们的潜在风险非常重要。为了确定浣熊携带抗菌素耐药性(AMR)和人类病原体的潜在风险,我们调查了生活在韩国城市森林地区和农村稻田地区的浣熊粪便抵抗组(抗菌素耐药性基因收集:ARGs)、致病基因组(毒力因子基因收集:vfg)和微生物组(细菌种类收集)的空间季节性格局。宏基因组测序结果显示,粪便抗性组、致病基因组和微生物组的组成均随季节变化而变化,我们推测这种变化与杂食性貉饮食的季节性变化有关。此外,尽管不像季节差异那么明显,但我们也观察到抗性组的地理差异,氨基糖苷抗性基因在农村地区更为普遍,这可能反映了抗菌素耐药性出现的选择压力的地理差异,例如在农业地区的水稻种植中使用可能含有抗菌素的粪便。此外,我们的网络分析显示,特定的抗微生物药物耐药性基因与特定的细菌病原体有关,例如,tetA-P与梭状芽胞杆菌有关。总体而言,我们的研究首次成功揭示了野生动物不仅微生物组存在时空变化,而且抵抗组和致病基因组也存在时空变化,并且浣熊携带的细菌致病菌的AMR风险具有空间季节性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Spatioseasonal Comparison of Fecal Resistome and Pathogenome of Raccoon Dogs in Korea.

The raccoon dog (Nyctereutes procyonoides) is a medium-sized omnivore native to Asia. Because they live close to human habitation, and therefore, there is likely bidirectional influence between raccoon dogs and humans, it is important to investigate their potential risks. Here, to identify potential risks of carriage of antimicrobial resistance (AMR) and human pathogens by raccoon dogs, we investigated spatioseasonal patterns of fecal resistome (collection of antimicrobial resistance genes: ARGs), pathogenome (collection of virulence factor genes: VFGs), and microbiome (collection of bacterial species) of raccoon dogs inhabiting an urban forest area and a rural rice paddy area in Korea. Metagenomic sequencing revealed that the compositions of fecal resistome, pathogenome, and microbiome were all patterned by season, and we hypothesize that the observed patterns are due to seasonal changes in the diet of omnivorous raccoon dogs. Furthermore, although less pronounced than the seasonal differences, we also observed the geographical differences in the resistome, with aminoglycoside resistance genes being more prevalent in the rural area, which may reflect the geographical difference in selective pressures for AMR emergence, such as the use of manure that may contain antimicrobials in rice cultivation in agricultural areas. Additionally, our network analysis revealed that specific antimicrobial resistance genes were linked to specific bacterial pathogens, e.g., tetA-P to Clostridium. Overall, our study successfully revealed for the first time that not only the microbiome, but also the resistome and pathogenome of wild animals change spatioseasonally, and that the risk of AMR in bacterial pathogens laden by raccoon dogs is spatioseasonal.

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来源期刊
Ecohealth
Ecohealth 环境科学-环境科学
CiteScore
4.50
自引率
4.00%
发文量
45
审稿时长
>24 weeks
期刊介绍: EcoHealth aims to advance research, practice, and knowledge integration at the interface of ecology and health by publishing high quality research and review articles that address and profile new ideas, developments, and programs. The journal’s scope encompasses research that integrates concepts and theory from many fields of scholarship (including ecological, social and health sciences, and the humanities) and draws upon multiple types of knowledge, including those of relevance to practice and policy. Papers address integrated ecology and health challenges arising in public health, human and veterinary medicine, conservation and ecosystem management, rural and urban development and planning, and other fields that address the social-ecological context of health. The journal is a central platform for fulfilling the mission of the EcoHealth Alliance to strive for sustainable health of people, domestic animals, wildlife, and ecosystems by promoting discovery, understanding, and transdisciplinarity. The journal invites substantial contributions in the following areas: One Health and Conservation Medicine o Integrated research on health of humans, wildlife, livestock and ecosystems o Research and policy in ecology, public health, and agricultural sustainability o Emerging infectious diseases affecting people, wildlife, domestic animals, and plants o Research and practice linking human and animal health and/or social-ecological systems o Anthropogenic environmental change and drivers of disease emergence in humans, wildlife, livestock and ecosystems o Health of humans and animals in relation to terrestrial, freshwater, and marine ecosystems Ecosystem Approaches to Health o Systems thinking and social-ecological systems in relation to health o Transdiiplinary approaches to health, ecosystems and society.
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